Nitric Oxide Inhibitory Carbazole Alkaloids from the Folk Medicine Murraya tetramera C.C. Huang

2020 ◽  
Vol 17 (11) ◽  
Author(s):  
Hai‐Ning Lyu ◽  
Ying Zhou ◽  
Ran Wen ◽  
Peng‐Fei Tu ◽  
Yong Jiang
Keyword(s):  
Nitric Oxide ◽  
Folk Medicine ◽  
Carbazole Alkaloids

scholarly journals Roles of Nitric Oxide and Prostaglandins in the Sustained Antihypertensive Effects of Acanthospermum hispidum DC. on Ovariectomized Rats with Renovascular Hypertension

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Rhanany Alan Calloi Palozi ◽  
Maysa Isernhagen Schaedler ◽  
Cleide Adriane Signor Tirloni ◽  
Aniely Oliveira Silva ◽  
Francislaine Aparecida dos Reis Lívero ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Renovascular Hypertension ◽  
Vascular Reactivity ◽  
Nitrosative Stress ◽  
Folk Medicine ◽  
Thiobarbituric Acid ◽  
Ovariectomized Rats ◽  
Oxidative And Nitrosative Stress ◽  
Antihypertensive Response

Although Acanthospermum hispidum is used in Brazilian folk medicine as an antihypertensive, no study evaluated its effects on a renovascular hypertension and ovariectomy model. So, this study investigated the mechanisms involved in the antihypertensive effects of an ethanol-soluble fraction obtained from A. hispidum (ESAH) using two-kidney-one-clip hypertension in ovariectomized rats (2K1C plus OVT). ESAH was orally administered at doses of 30, 100, and 300 mg/kg, daily, for 28 days, after 5 weeks of surgery. Enalapril (15 mg/kg) and hydrochlorothiazide (25 mg/kg) were used as standard drugs. Diuretic activity was evaluated on days 1, 7, 14, 21, and 28. Systolic, diastolic, and mean blood pressure and heart rate were recorded. Serum creatinine, urea, thiobarbituric acid reactive substances, nitrosamine, nitrite, aldosterone, vasopressin levels, and ACE activity were measured. The vascular reactivity and the role of nitric oxide (NO) and prostaglandins (PG) in the vasodilator response of ESAH on the mesenteric vascular bed (MVB) were also investigated. ESAH treatment induced an important saluretic and antihypertensive response, therefore recovering vascular reactivity in 2K1C plus OVT-rats. This effect was associated with a reduction of oxidative and nitrosative stress with a possible increase in the NO bioavailability. Additionally, a NO and PG-dependent vasodilator effect was observed on the MEV.

Anti-Oxidative and Anti-Inflammatory Effects of Lobelia chinensis In Vitro and In Vivo

2015 ◽  
Vol 43 (02) ◽  
pp. 269-287 ◽  
Author(s):  
Kun-Cheng Li ◽  
Yu-Ling Ho ◽  
Guan-Jhong Huang ◽  
Yuan-Shiun Chang
Keyword(s):  
Nitric Oxide ◽  
Folk Medicine ◽  
Ethyl Acetate Fraction ◽  
Raw264.7 Macrophages ◽  
Tnf Α ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Factor Α

Lobelia chinensis Lour (LcL) is a popular herb that has been widely used as folk medicine in China for the treatment of fever, lung cancer, and inflammation for hundreds of years. Recently, several studies have shown that the anti-inflammatory properties were correlated with the inhibition of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from the NF-κB pathway. The aim of this study was to evaluate the anti-oxidative and anti-inflammatory activities of L. chinensis. Both suppressive activities on LPS-induced nitric oxide production in RAW264.7 macrophages in vitro and the acute rat lung injury model in vivo were studied. The results showed that the methanol extract of LcL and its fractions within the range of 62.5–250 μg/mL did not induce cytotoxicity (p < 0.001). The ethyl acetate fraction of LcL showed better NO inhibition activity than other fractions. On the other hand, the Lc-EA (62.5, 125, 250 mg/kg) pretreated rats showed a decrease in the pro-inflammatory cytokines (TNF-α, IL-β, IL-6) and inhibited iNOS, COX-2 expression through the NF-κB pathway. These results suggested that L. chinensis exhibited an anti-inflammatory effect through the NF-κB pathways.

scholarly journals Nitric oxide (NO) radical inhibitory of hedyotis philippinensis and its marker compound, asperuloside

2014 ◽  
Vol 10 (1) ◽  
Author(s):  
Emi Norzehan Mohamad Mahbob ◽  
Rohaya Ahmad ◽  
Syahida Ahmad
Keyword(s):  
Nitric Oxide ◽  
Folk Medicine ◽  
No Production ◽  
Marker Compound ◽  
Methanolic Extracts ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Raw264.7 Macrophage

Hedyotis spesies have been used in Chinese folk medicine as a treatment for colds, stomatitis and various inflammations. In this study, three methanolic extracts of Hedyotis species (Hedyotis corymbosa, Hedyotis havilandii and Hedyotis philippinensis) were screened for their anti-inflammatory activity. The plant extracts along with asperuloside, the marker compound of H. philippinensis were tested for their anti-inflammatory effect against lipopolysaccharide (LPS) and interferon- (IFN-)-induced nitric oxide (NO) production using RAW264.7 macrophage cells. Among the three species, H. philippinensis (leaves and stems) showed good NO radical inhibitory activity (with IC50 values of 139.76±12.50 and 176.21±2.48 g/ml, respectively). Asperuloside, isolated as a major compound from the plant moderately inhibited LPS/IFN--induced NO production by 63% at a concentration of 100 M (45% cell viability) compared to L-NAME (77 %). Its IC50 value was found to be 75.45±2.25 M. Its inhibition was also found to be dose-dependent.

scholarly journals A DESCRIPTIVE STUDY AND IN-VITRO ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF TRIDAX PROCUMBENS LINN

2021 ◽  
Vol 11 (6) ◽  
pp. 1-4
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ferric Chloride ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Alcoholic Extract ◽  
Tridax Procumbens

Tridax Procumbens Linn is a member of the Asteraceae family. Tridax Procumbens has to be utilized because native medication intended for a number of complaints and problems in humans and animals for thousands of years. It is used widely in Indian conventional remedies for healing of wounds, like anticoagulants, in fungal infection, in diarrhea and dysentery, as an antioxidant, antimicrobial, anti-inflammatory, and immunomodulators. In folk medicine, certain communicable skin diseases are treated by using leaves extract. It's also known as 'Bhringraj,' an ayurvedic drug used to treat liver problems. At least 12,000 people have been separated from their families. These compounds protect plants from pathogens, insects, and herbivores by acting as defensive mechanisms. The aim of the test is to establish the antioxidant potential of the leaves of Tridax Procumbens. The current research is aimed at identifying novel plant directions, and antioxidant activity has been chosen for that reason. Using the maceration procedure, the power of the plant's shade dried leaves was extracted with chloroform water and ethanol. The antioxidant activities of the resulting extracts were evaluated using 2 techniques: nitric oxide scavenging activity and ferric chloride reductive ability. The alcoholic extract in 600 mg/ml and 800 mg/ml and 1000 mg/ml concentration has demonstrated antioxidant activity higher than ascorbic acid (20 mg) by nitric oxide scavenging method. By using a ferric chloride scavenging model, the aqueous and alcoholic extracts at 400 g/ml and 600 g/ml concentrations revealed antioxidant activity near to that of ascorbic acid (20 g).

scholarly journals A DESCRIPTIVE STUDY AND IN-VITRO ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF TRIDAX PROCUMBENS LINN

2021 ◽  
Vol IC1 (1) ◽  
pp. 1-4
Author(s):  
Hemant P. Suryawanshi
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ferric Chloride ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Alcoholic Extract ◽  
Tridax Procumbens

Tridax Procumbens Linn is a member of the Asteraceae family. Tridax Procumbens has to be utilized because native medication intended for a number of complaints and problems in humans and animals for thousands of years. It is used widely in Indian conventional remedies for healing of wounds, like anticoagulants, in fungal infection, in diarrhea and dysentery, as an antioxidant, antimicrobial, anti-inflammatory, and immunomodulators. In folk medicine, certain communicable skin diseases are treated by using leaves extract. It's also known as 'Bhringraj,' an ayurvedic drug used to treat liver problems. At least 12,000 people have been separated from their families. These compounds protect plants from pathogens, insects, and herbivores by acting as defensive mechanisms. The aim of the test is to establish the antioxidant potential of the leaves of Tridax Procumbens. The current research is aimed at identifying novel plant directions, and antioxidant activity has been chosen for that reason. Using the maceration procedure, the power of the plant's shade dried leaves was extracted with chloroform water and ethanol. The antioxidant activities of the resulting extracts were evaluated using 2 techniques: nitric oxide scavenging activity and ferric chloride reductive ability. The alcoholic extract in 600 mg/ml and 800 mg/ml and 1000 mg/ml concentration has demonstrated antioxidant activity higher than ascorbic acid (20 mg) by nitric oxide scavenging method. By using a ferric chloride scavenging model, the aqueous and alcoholic extracts at 400 g/ml and 600 g/ml concentrations revealed antioxidant activity near to that of ascorbic acid (20 g).

Inhibitory Effect of Spirodela polyrhixa on the Secretion of NO in LPS-stimulated Macrophages

2004 ◽  
Vol 32 (01) ◽  
pp. 65-73 ◽  
Author(s):  
Woo Shin Ko ◽  
Young Hee Kim ◽  
Jung Won Yoon ◽  
So Woon Yoon ◽  
Han Do Kim
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Diseases ◽  
Folk Medicine ◽  
Inhibitory Effect ◽  
Peritoneal Macrophages ◽  
No Production ◽  
Inhibitory Protein ◽  
Transient Transfection Assay ◽  
Inflammatory Parameters ◽  
Oxide Synthase

Spirodela polyrhixa Schleid has been used in folk medicine to treat inflammatory diseases. Since nitric oxide (NO) is one of the major inflammatory parameters, we studied the effect of aqueous extracts of Spirodela polyrhixa (AESP) on NO production in lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages. AESP inhibited the secretion of NO in macrophages, without affecting cell viability. The protein level of inducible nitric oxide synthase (iNOS) in peritoneal macrophages was also decreased by AESP. Transient transfection assay of reporter plasmid and gel shift assay indicated that AESP blocked the activation of nuclear factor-kappa B (NF-κB), which was considered to be a potential transcription factor for iNOS expression. AESP also blocked the phosphorylation and degradation of inhibitory protein I kappa B-alpha (IκB-α). These results suggest that AESP could exert its anti-inflammatory actions by suppressing the synthesis of NO through inhibition of NF-κB activity.

scholarly journals Identification of Anti-Inflammatory Compounds from Hawaiian Noni (Morinda citrifolia L.) Fruit Juice

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4968
Author(s):  
Dahae Lee ◽  
Jae Sik Yu ◽  
Peng Huang ◽  
Mallique Qader ◽  
Arulmani Manavalan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fruit Juice ◽  
Inflammatory Diseases ◽  
Folk Medicine ◽  
Morinda Citrifolia ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Phytochemical Analysis ◽  
No Production ◽  
Anti Inflammatory

Noni (Morinda citrifolia L.) fruit juice has been used in Polynesia as a traditional folk medicine and is very popular worldwide as a functional food supplement. In this study, compounds present in Hawaiian Noni fruit juice, with anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were identified. Five compounds were isolated using a bioassay-driven technique and phytochemical analysis of noni fruit juice: asperulosidic acid (1), rutin (2), nonioside A (3), (2E,4E,7Z)-deca-2,4,7-trienoate-2-O-β-d-glucopyranosyl-β-d-glucopyranoside (4), and tricetin (5). The structures of these five compounds were determined via NMR spectroscopy and LC/MS. In an anti-inflammatory assay, compounds 1–5 inhibited the production of nitric oxide (NO), which is a proinflammatory mediator, in LPS-stimulated macrophages. Moreover, the mechanisms underlying the anti-inflammatory effects of compounds 1–5 were investigated. Parallel to the inhibition of NO production, treatment with compounds 1–5 downregulated the expression of IKKα/β, I-κBα, and NF-κB p65 in LPS-stimulated macrophages. Furthermore, treatment with compounds 1–5 downregulated the expression of nitric oxide synthase and cyclooxygenase-2. Thus, these data demonstrated that compounds 1–5 present in noni fruit juice, exhibited potential anti-inflammatory activity; these active compounds may contribute preventively and therapeutically against inflammatory diseases.

scholarly journals ANTIOXIDANT AND ANTI-INFLAMMATORY PROPERTIES OF PTEROSPERMUM RUBIGINOSUM HEYNE EX WIGHT AND ARN AND PTEROSPERMUM RETICULATUM WIGHT AND ARN (STERCULIACEAE): AN IN VITRO COMPARATIVE STUDY

2019 ◽  
pp. 272-275 ◽  
Author(s):  
JENSON JACOB ◽  
SREEJITH K
Keyword(s):  
Nitric Oxide ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protease Inhibition ◽  
Nitric Oxide Radical ◽  
The Family ◽  
Anti Inflammatory ◽  
Reducing Power Assay

Objectives: Plants from the family Sterculiaceae are used as folk medicine for treating various diseases in India. This study aims to determine the antioxidant and anti-inflammatory properties of Pterospermum rubiginosum and Pterospermum reticulatum of the family Sterculiaceae. The barks of P. rubiginosum and P. reticulatum are used in traditional medicine especially in the treatment of wounds, sprains, bone fracture, etc. This study, we compare the antioxidant and anti-inflammatory potentials of the stem bark of these two plants. Methods: The free radical scavenging assays such as 2,2–diphenyl,1–picrylhydrazyl (DPPH), 2,2’–azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS), hydroxyl radical, nitric oxide radical, phosphormolybdenum assay, and reducing power assay are used for the measurement of antioxidant potentials. The in vitro anti-inflammatory activities of the extracts are evaluated by means of lipoxygenase (LOX) and protease inhibition. Results: Both P. rubiginosum and P. reticulatum scavenge DPPH (70.10% and 91.02%), ABTS (94.48 and 98.19%), hydroxy (76.02 and 87.67%), and nitric oxide (87.02 and 80.84%) radicals. Phosphomolybdenum assay and reducing power assay, used for the measurement of antioxidant potentials also showed good results. Regarding the anti-inflammatory potential, the methanolic extract of the plants shows anti-protease activity (51.29 and 64.93%) and anti-LOX activity (56%) while P. rubiginosum does not exhibit anti-LOX activity. Conclusion: The above results demonstrate that the plants P. rubiginosum and P. reticulatum are rich source of antioxidant and anti-inflammatory compounds and it is the first report on theantioxidant and anti-inflammatory properties of the barks of these plants.

Localization of endothelial nitric oxide synthase in the normal and failing human atrial myocardium

1996 ◽  
Vol 54 ◽  
pp. 786-787
Author(s):  
Chi-Ming Wei ◽  
Margarita Bracamonte ◽  
Shi-Wen Jiang ◽  
Richard C. Daly ◽  
Christopher G.A. McGregor ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Heart Failure ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Cardiac Tissues ◽  
Mammalian Tissues ◽  
End Stage Heart Failure ◽  
End Stage ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Nitric oxide (NO) is a potent endothelium-derived relaxing factor which also may modulate cardiomyocyte inotropism and growth via increasing cGMP. While endothelial nitric oxide synthase (eNOS) isoforms have been detected in non-human mammalian tissues, expression and localization of eNOS in the normal and failing human myocardium are poorly defined. Therefore, the present study was designed to investigate eNOS in human cardiac tissues in the presence and absence of congestive heart failure (CHF).Normal and failing atrial tissue were obtained from six cardiac donors and six end-stage heart failure patients undergoing primary cardiac transplantation. ENOS protein expression and localization was investigated utilizing Western blot analysis and immunohistochemical staining with the polyclonal rabbit antibody to eNOS (Transduction Laboratories, Lexington, Kentucky).

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