MsrA Efflux Pump Inhibitory Activity of Piper cubeba L.f. and its Phytoconstituents against Staphylococcus aureus RN4220

2020 ◽  
Vol 17 (8) ◽  
Author(s):  
Pallavi Ahirrao ◽  
Rushikesh Tambat ◽  
Nishtha Chandal ◽  
Nisha Mahey ◽  
Anjoo Kamboj ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Activity ◽  
Efflux Pump

Efflux pump inhibitory activity of flavonoids isolated from Alpinia calcarata against methicillin-resistant Staphylococcus aureus

Biologia ◽  
2016 ◽  
Vol 71 (5) ◽  
Author(s):  
Harmandeep K. Randhawa ◽  
Kanwarpreet K. Hundal ◽  
Pallavi N. Ahirrao ◽  
Sanjay M. Jachak ◽  
Hemraj S. Nandanwar
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pump ◽  
Methicillin Resistant

AbstractMethicillin-resistant

scholarly journals Novel inhibitory activity of the Staphylococcus aureus NorA efflux pump by a kaempferol rhamnoside isolated from Persea lingue Nees

2012 ◽  
Vol 67 (5) ◽  
pp. 1138-1144 ◽  
Author(s):  
J. G. Holler ◽  
S. B. Christensen ◽  
H.-C. Slotved ◽  
H. B. Rasmussen ◽  
A. Guzman ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Activity ◽  
Efflux Pump

scholarly journals SYNERGISTIC AND EFFLUX PUMP INHIBITORY ACTIVITY OF PLANT EXTRACTS AND ANTIBIOTICS ON STAPHYLOCOCCUS AUREUS STRAINS

2016 ◽  
pp. 277
Author(s):  
Urmila Sharma ◽  
Savita Jandaik ◽  
Jyoti Mehta ◽  
Manindra Mohan
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Efflux Pump ◽  
Western Himalaya ◽  
Diffusion Method ◽  
Synergistic Activity ◽  
Plant Materials ◽  
Activity Maximum

<p>ABSTRACT<br />Objective: The purpose of this study was to evaluate the synergistic and efflux pump inhibitory activities of some important medicinal plants collected<br />from Western Himalaya against different strains of Staphylococcus aureus to facilitate the reintroduction of therapeutically ineffective antibiotics back<br />into clinical use.<br />Methods: Various plant materials were extracted using methanol, ethyl acetate, and water. The dried extracts were screened for synergistic activity<br />by well diffusion method, and efflux pump inhibitory activity for plants extracts exhibiting maximum synergism assessed by berberine uptake assay<br />and ethidium bromide efflux inhibition assay, respectively.<br />Results: Methanolic extract (ME) and ethyl acetate extracts (EE) of most of the plant extracts showed synergistic activity with ciprofloxacin and<br />norfloxacin, whereas an aqueous extract showed no any synergistic activity. Maximum efflux pump inhibitory activity was observed for ME of Angelica<br />glauca followed by EE of Ficus carica.<br />Conclusion: ME of A. glauca exhibited potentials of efflux pump inhibition, and thus can, be used as an adjuvant with antibiotics to overcome the<br />problem of drug resistance due to efflux.<br />Keywords: Staphylococcus aureus, Therapeutically ineffective antibiotics, Synergistic effect, Efflux pump inhibitors.</p><p> </p>

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

Virtual Screening for Novel Staphylococcus Aureus NorA Efflux Pump Inhibitors From Natural Products

2015 ◽  
Vol 11 (2) ◽  
pp. 135-155 ◽  
Author(s):  
Khac-Minh Thai ◽  
Trieu-Du Ngo ◽  
Thien-Vy Phan ◽  
Thanh-Dao Tran ◽  
Ngoc-Vinh Nguyen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Natural Products ◽  
Virtual Screening ◽  
Efflux Pump ◽  
Efflux Pump Inhibitors

Inhibitory Activity of Brown Propolis Extracts on a Norfloxacin-Resistant Strain of Staphylococcus aureus

2021 ◽  
Author(s):  
Vanessa Moreira Frota ◽  
Francisco Matheus F. Dias ◽  
Mariana Ferreira do Nascimento ◽  
Lavosyer da Silva Mendonça ◽  
Emanuella Cristina dos Santos Moita ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Activity ◽  
Resistant Strain

scholarly journals Genome-Wide Identification of Resveratrol Intrinsic Resistance Determinants in Staphylococcus aureus

Antibiotics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 82
Author(s):  
Liping Liu ◽  
Hanne Ingmer ◽  
Martin Vestergaard
Keyword(s):  
Staphylococcus Aureus ◽  
Stress Response ◽  
Inhibitory Activity ◽  
Bacterial Species ◽  
Dna Integrity ◽  
Intrinsic Resistance ◽  
Potential Health ◽  
Cellular Effects ◽  
Development Of Resistance ◽  
Wide Range

Resveratrol has been extensively studied due to its potential health benefits in multiple diseases, for example, cancer, obesity and cardiovascular diseases. Besides these properties, resveratrol displays inhibitory activity against a wide range of bacterial species; however, the cellular effects of resveratrol in bacteria remain incompletely understood, especially in the human pathogen, Staphylococcus aureus. In this study, we aimed to identify intrinsic resistance genes that aid S. aureus in tolerating the activity of resveratrol. We screened the Nebraska Transposon Mutant Library, consisting of 1920 mutants with inactivation of non-essential genes in S. aureus JE2, for increased susceptibly to resveratrol. On agar plates containing 0.5× the minimum inhibitory concentration (MIC), 17 transposon mutants failed to grow. Of these, four mutants showed a two-fold reduction in MIC, being the clpP protease mutant and three mutants with deficiencies in the electron transport chain (menD, hemB, aroC). The remaining 13 mutants did not show a reduction in MIC, but were confirmed by spot-assays to have increased susceptibility to resveratrol. Several genes were associated with DNA damage repair (recJ, xerC and xseA). Treatment of S. aureus JE2 with sub-inhibitory concentrations of resveratrol did not affect the expression of recJ, xerC and xseA, but increased expression of the SOS–stress response genes lexA and recA, suggesting that resveratrol interferes with DNA integrity in S. aureus. Expression of error-prone DNA polymerases are part of the SOS–stress response and we could show that sub-inhibitory concentrations of resveratrol increased overall mutation frequency as measured by formation of rifampicin resistant mutants. Our data show that DNA repair systems are important determinants aiding S. aureus to overcome the inhibitory activity of resveratrol. Activation of the SOS response by resveratrol could potentially facilitate the development of resistance towards conventional antibiotics in S. aureus.

scholarly journals Staphylococcus aureus Mutants Isolated via Exposure to Nonfluorinated Quinolones: Detection of Known and Unique Mutations

2001 ◽  
Vol 45 (12) ◽  
pp. 3422-3426 ◽  
Author(s):  
Siddhartha Roychoudhury ◽  
Tracy L. Twinem ◽  
Kelly M. Makin ◽  
Mark A. Nienaber ◽  
Chuiying Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sequence Analysis ◽  
Efflux Pump ◽  
Serial Passage ◽  
Efflux Pump Inhibitor ◽  
In Vitro Development ◽  
Development Of Resistance ◽  
High Level ◽  
Vitro Development

ABSTRACT The in vitro development of resistance to the new nonfluorinated quinolones (NFQs; PGE 9262932, PGE 4175997, and PGE 9509924) was investigated in Staphylococcus aureus. At concentrations two times the MIC, step 1 mutants were isolated more frequently with ciprofloxacin and trovafloxacin (9.1 × 10−8 and 5.7 × 10−9, respectively) than with the NFQs, gatifloxacin, or clinafloxacin (<5.7 × 10−10). Step 2 and step 3 mutants were selected via exposure of a step 1 mutant (selected with trovafloxacin) to four times the MICs of trovafloxacin and PGE 9262932. The step 1 mutant contained the known Ser80-Phe mutation in GrlA, and the step 2 and step 3 mutants contained the known Ser80-Phe and Ser84-Leu mutations in GrlA and GyrA, respectively. Compared to ciprofloxacin, the NFQs were 8-fold more potent against the parent and 16- to 128-fold more potent against the step 3 mutants. Mutants with high-level NFQ resistance (MIC, 32 μg/ml) were isolated by the spiral plater-based serial passage technique. DNA sequence analysis of three such mutants revealed the following mutations: (i) Ser84-Leu in GyrA and Glu84-Lys and His103-Tyr in GrlA; (ii) Ser-84Leu in GyrA, Ser52-Arg in GrlA, and Glu472-Val in GrlB; and (iii) Ser84-Leu in GyrA, Glu477-Val in GyrB, and Glu84-Lys and His103-Tyr in GrlA. Addition of the efflux pump inhibitor reserpine (10 μg/ml) resulted in 4- to 16-fold increases in the potencies of the NFQs against these mutants, whereas it resulted in 2-fold increases in the potencies of the NFQs against the parent.

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