scholarly journals Lipophilic 9,10‐Dehydrofukinone Action on Pathogenic and Non‐Pathogenic Bacterial Biofilms. Why Is This Main Volatile Metabolite in Senecio ?

2020 ◽  
Vol 17 (6) ◽  
Author(s):  
María C. Verni ◽  
José A. Garay ◽  
Lucía Mendoza ◽  
Alicia Bardón ◽  
Susana Borkosky ◽  
...  
Keyword(s):  
Bacterial Biofilms ◽  
Volatile Metabolite

Lipids and Free Fatty Acids of Red Sea Avrainvillea amadelpha, Holothuria atra, and Sarcocornia fruticosa Inhibit Marine Bacterial Biofilms

2020 ◽  
Vol 17 (6) ◽  
pp. 466-471
Author(s):  
Usama W. Hawas ◽  
Fekri Shaher ◽  
Mohamed Ghandourah ◽  
Lamia T. Abou El-Kassem ◽  
Sathianeson Satheesh ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Red Sea ◽  
Inhibitory Activity ◽  
Sea Cucumber ◽  
Bacterial Biofilms ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Holothuria Atra ◽  
Sarcocornia Fruticosa

This study aimed at evaluating the antibiofilm activity of the Red Sea metabolites from green alga Avrainvillea amadelpha, sea cucumber Holothuria atra and costal plant Sarcocornia fruticosa against three biofilm bacterial strains isolated from Jeddah coast. Free fatty acids (FFAs) and other lipoidal matters were extracted from these organisms and analyzed by GC-MS. The composition of lipoidal fractions showed that A. amadelpha is rich by 74% saturated FAs, while sea cucumber H. atra revealed high content (60%) of unsaturated FAs. Palmitic acid is the major FA component in all species ranging from 14.5 to 26.7%. Phytol, sterols and hydrocarbons (C8-C29) were represented in the alga A. amadelpha as high contents with values 25.8, 21.9 and 18.5%, respectively. The extracts and lipoidal contents showed biofilm inhibitory activity against the isolated bacterial strains, where the unsaponified lipoidal fraction of S. fruticosa exhibited highest inhibitory activity against Planomicrobium sp. at concentration of 200 µg/mL.

GMP-Like Benzimidazole Broadly Inhibits Bacterial Biofilms

2011 ◽  
Vol 6 (11) ◽  
pp. 470-471
Author(s):  
David C. Holzman
Keyword(s):  
Bacterial Biofilms

The influence of fluid shear on the structure and material properties of sulphate-reducing bacterial biofilms

2002 ◽  
Vol 29 (6) ◽  
pp. 347-353 ◽  
Author(s):  
B C Dunsmore ◽  
A Jacobsen ◽  
L Hall-Stoodley ◽  
C J Bass ◽  
H M Lappin-Scott ◽  
...  
Keyword(s):  
Material Properties ◽  
Bacterial Biofilms ◽  
Fluid Shear

scholarly journals Pseudomonas aeruginosa PA14 Enhances the Efficacy of Norfloxacin against Staphylococcus aureus Newman Biofilms

2020 ◽  
Vol 202 (18) ◽  
Author(s):  
Giulia Orazi ◽  
Fabrice Jean-Pierre ◽  
George A. O’Toole
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Respiratory Infections ◽  
Multiple Infection ◽  
Bacterial Biofilms ◽  
Interspecies Interactions ◽  
Chronic Infections ◽  
Content Type

ABSTRACT The thick mucus within the airways of individuals with cystic fibrosis (CF) promotes frequent respiratory infections that are often polymicrobial. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most prevalent pathogens that cause CF pulmonary infections, and both are among the most common etiologic agents of chronic wound infections. Furthermore, the ability of P. aeruginosa and S. aureus to form biofilms promotes the establishment of chronic infections that are often difficult to eradicate using antimicrobial agents. In this study, we found that multiple LasR-regulated exoproducts of P. aeruginosa, including 2-heptyl-4-hydroxyquinoline N-oxide (HQNO), siderophores, phenazines, and rhamnolipids, likely contribute to the ability of P. aeruginosa PA14 to shift S. aureus Newman norfloxacin susceptibility profiles. Here, we observe that exposure to P. aeruginosa exoproducts leads to an increase in intracellular norfloxacin accumulation by S. aureus. We previously showed that P. aeruginosa supernatant dissipates the S. aureus membrane potential, and furthermore, depletion of the S. aureus proton motive force recapitulates the effect of the P. aeruginosa PA14 supernatant on shifting norfloxacin sensitivity profiles of biofilm-grown S. aureus Newman. From these results, we hypothesize that exposure to P. aeruginosa PA14 exoproducts leads to increased uptake of the drug and/or an impaired ability of S. aureus Newman to efflux norfloxacin. Surprisingly, the effect observed here of P. aeruginosa PA14 exoproducts on S. aureus Newman susceptibility to norfloxacin seemed to be specific to these strains and this antibiotic. Our results illustrate that microbially derived products can alter the ability of antimicrobial agents to kill bacterial biofilms. IMPORTANCE Pseudomonas aeruginosa and Staphylococcus aureus are frequently coisolated from multiple infection sites, including the lungs of individuals with cystic fibrosis (CF) and nonhealing diabetic foot ulcers. Coinfection with P. aeruginosa and S. aureus has been shown to produce worse outcomes compared to infection with either organism alone. Furthermore, the ability of these pathogens to form biofilms enables them to cause persistent infection and withstand antimicrobial therapy. In this study, we found that P. aeruginosa-secreted products dramatically increase the ability of the antibiotic norfloxacin to kill S. aureus biofilms. Understanding how interspecies interactions alter the antibiotic susceptibility of bacterial biofilms may inform treatment decisions and inspire the development of new therapeutic strategies.

scholarly journals Biofilm Lithography enables high-resolution cell patterning via optogenetic adhesin expression

2018 ◽  
Vol 115 (14) ◽  
pp. 3698-3703 ◽  
Author(s):  
Xiaofan Jin ◽  
Ingmar H. Riedel-Kruse
Keyword(s):  
Escherichia Coli ◽  
Cell Patterning ◽  
Biophysical Model ◽  
Microbial Consortia ◽  
Bacterial Biofilms ◽  
Surface Pretreatment ◽  
Resolution Cell ◽  
Stimulation Of

Bacterial biofilms represent a promising opportunity for engineering of microbial communities. However, our ability to control spatial structure in biofilms remains limited. Here we engineerEscherichia coliwith a light-activated transcriptional promoter (pDawn) to optically regulate expression of an adhesin gene (Ag43). When illuminated with patterned blue light, long-term viable biofilms with spatial resolution down to 25 μm can be formed on a variety of substrates and inside enclosed culture chambers without the need for surface pretreatment. A biophysical model suggests that the patterning mechanism involves stimulation of transiently surface-adsorbed cells, lending evidence to a previously proposed role of adhesin expression during natural biofilm maturation. Overall, this tool—termed “Biofilm Lithography”—has distinct advantages over existing cell-depositing/patterning methods and provides the ability to grow structured biofilms, with applications toward an improved understanding of natural biofilm communities, as well as the engineering of living biomaterials and bottom–up approaches to microbial consortia design.

scholarly journals Milieu matters: An in vitro wound milieu to recapitulate key features of, and probe new insights into, mixed-species bacterial biofilms

Biofilm ◽  
2021 ◽  
pp. 100047
Author(s):  
Snehal Kadam ◽  
Vandana Madhusoodhanan ◽  
Radhika Dhekane ◽  
Devyani Bhide ◽  
Rutuja Ugale ◽  
...  
Keyword(s):  
Bacterial Biofilms ◽  
Mixed Species ◽  
Key Features

Enhanced efficacy against bacterial biofilms via host:guest cyclodextrin‐doxycycline inclusion complexes

2021 ◽  
Author(s):  
Pedro Pires Goulart Guimarães ◽  
Andressa Coelho de Menezes ◽  
Karina Imaculada Rosa Teixeira ◽  
Ângelo M. L. Denadai ◽  
Richard A. Fills ◽  
...  
Keyword(s):  
Inclusion Complexes ◽  
Bacterial Biofilms
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