Effect ofCostus spiralis(Jacq.)RoscoeLeaves, Methanolic Extract and Guaijaverin on Blood Glucose and Lipid Levels in a Type II Diabetic Rat Model

2019 ◽  
Vol 16 (1) ◽  
pp. e1800365
Author(s):  
Regiane C. Duarte ◽  
Silvia H. Taleb-Contini ◽  
Paulo S. Pereira ◽  
Camila F. Oliveira ◽  
Carlos Eduardo S. Miranda ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Rat Model ◽  
Methanolic Extract ◽  
Diabetic Rat ◽  
Type Ii ◽  
Lipid Levels ◽  
Diabetic Rat Model

Exploration of the Effect and Mechanism of ShenQi Compound in a Spontaneous Diabetic Rat Model

2019 ◽  
Vol 19 (5) ◽  
pp. 622-631 ◽  
Author(s):  
Ya Liu ◽  
Jian Kang ◽  
Hong Gao ◽  
Xiyu Zhang ◽  
Jun Chao ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Signaling Pathway ◽  
Rat Model ◽  
Mtor Signaling ◽  
Diabetic Rat ◽  
Gene Microarray ◽  
Mtor Signaling Pathway ◽  
Glucose Fluctuation ◽  
Blood Glucose Fluctuation ◽  
Diabetic Rat Model

Background: Type 2 Diabetes Mellitus (T2DM) is a world-wide metabolic disease with no cure from drugs and treatment. In China, The Traditional Chinese Medicine (TCM) herbal formulations have been used to treat T2DM for centuries. Methods: In this study, we proposed a formula called ShenQi Compound (SQC), which has been used in clinical therapeutics in China for several years. We evaluated the effect of SQC in a spontaneous diabetic rat model (GK rats) by detecting a series of blood indicators and performing histological observations. Meanwhile, the gene microarray and RT-qPCR experiments were used to explore the molecular mechanism of SQC treatment. In addition, western medicine, sitagliptin was employed as a comparison. Results: The results indicated that SQC and sitagliptin could effectively improve the serum lipid (blood Total Cholesterol (TC) and blood Triglycerides (TG)), hormone levels (serum insulin (INS), Glucagon (GC) and Glucagon-Like Peptide-1 (GLP-1)), alleviated the inflammatory response (hypersensitive C-Reactive Protein (hsCRP)), blood glucose fluctuation (Mean Blood Glucose (MBG), standard deviation of blood glucose (SDBG) and Largest Amplitude of plasma Glucose Excursions (LAGE)), pancreatic tissue damage and vascular injury for T2DM. Compared with sitagliptin, SQC achieved a better effect on blood glucose fluctuation (p<0.01). Meanwhile, the gene microarray and RT-qPCR experiments indicated that SQC and sitagliptin may improve the T2DM through affecting the biological functions related to apoptosis and circadian rhythm. Moreover, SQC might be able to influence the mTOR signaling pathway by regulating Pik3r1, Ddit4 expression. Conclusion: All these results indicate that SQC is an effective therapeutic drug on T2DM. Notably, SQC presents an obvious blood glucose fluctuation-preventing ability, which might be derived from the regulation of the mTOR signaling pathway.

scholarly journals Shenqi Jiangtang Granule Ameliorates Kidney Function by Inhibiting Apoptosis in a Diabetic Rat Model

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Qian Zhang ◽  
Xinhua Xiao ◽  
Jia Zheng ◽  
Ming Li ◽  
Miao Yu ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Serum Creatinine ◽  
Kidney Function ◽  
Rat Model ◽  
Urinary Albumin ◽  
Diabetic Group ◽  
Diabetic Rat ◽  
Apoptosis Pathway ◽  
A Genome ◽  
Diabetic Rat Model

Diabetic nephropathy (DN) is a major microvascular complication of diabetes. In addition to moderating hyperglycemia, Shenqi Jiangtang Granule (SJG) had a beneficial effect on kidney function in a clinical trial. However, the mechanism involved remains unclear. This study was conducted to identify the underlying molecular mechanisms. A diabetic rat model was generated by using a high-fat diet and streptozotocin (STZ) injection. Then, rats were given SJG at dosages of 400 mg/kg/d or 800 mg/kg/d by gavage for 8 weeks. After 8 weeks of treatment, blood glucose, serum creatinine, blood urea nitrogen (BUN), and 24-h urinary albumin were measured. Histochemical staining and TdT-mediated dUTP nick-end labeling (TUNEL) assays were performed in kidney. Kidney genomic expression in the SJG-treated group and diabetic group was detected by using a genome expression microarray. We found that SJG treatment reduced blood glucose, serum creatinine, BUN, and 24-h urinary albumin and affected kidney histology. The gene array revealed that the expression of 99 genes increased and the expression of 91 genes decreased in the HSJG group, compared with those of in the diabetic group. Pathway and gene ontology analysis of the differentially expressed genes showed an enrichment of the apoptosis pathway. SJG treatment reduced TUNEL- and caspase-3-positive cells in diabetic kidneys. SJG upregulated Bcl-2 and regucalcin expressions and reduced casp3 and Apaf1 expressions in diabetic rats. Our results suggest that SJG exerts a renal protective effect through the inhibition of cell apoptosis in a diabetic rodent model.

Effect of Curcumin on the Level of Blood Lipid and Blood Glucose in Diabetic Rat Model

2014 ◽  
Vol 1051 ◽  
pp. 419-422
Author(s):  
Ming San Miao ◽  
Lin Guo ◽  
Shuo Tian ◽  
Tan Wang
Keyword(s):  
Blood Glucose ◽  
Rat Model ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Lipoprotein Cholesterol ◽  
Diabetic Rat ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetic Rat Model

Objective: To investigate the effects of curcumin on blood lipid and blood glucose levels and its mechanism in diabetic rat model. Method: After streptozotocin (STZ) induced diabetic rat model, large, medium and small doses of curcumin group were partly given curcumin solution 400,200,100mg·kg-1, administered once a day, continuously 30 days. In 30 th day, determine blood glucose (BG) value, after the last injection , determine the serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) levels. Results: Compared with the model group rats, each dose of curcumin group rats’ BG, TC, TG and LDL-C levels were significantly decreased, HDL-C levels was increased significantly. Conclusion: Curcumin has a certain impact on blood lipids and blood glucose in diabetic rat model.

428: A Type II Diabetic Rat Model of Diabetic Voiding Dysfunction

2007 ◽  
Vol 177 (4S) ◽  
pp. 143-143
Author(s):  
Surat Phonsombat ◽  
Kavirach Tantiwongse ◽  
Thomas M. Fandel ◽  
Guifang Wang ◽  
Guiting Lin ◽  
...  
Keyword(s):  
Rat Model ◽  
Voiding Dysfunction ◽  
Diabetic Rat ◽  
Type Ii ◽  
Diabetic Rat Model

Abstract P243: A New Type 2 Diabetic Rat Model That is Associated with Cognitive Impairment in Aging

Hypertension ◽  
2016 ◽  
Vol 68 (suppl_1) ◽  
Author(s):  
Wenshan Lv ◽  
Hongwei Yu ◽  
Longyang Li ◽  
Christine Taylor ◽  
Ezekiel Gonzalez-Fernandez ◽  
...  
Keyword(s):  
Cognitive Impairment ◽  
Rat Model ◽  
Amyloid Β ◽  
Diabetic Rat ◽  
Type Ii ◽  
Sd Rats ◽  
Increased Risk ◽  
New Type ◽  
Diabetic Rat Model ◽  
Cerebral Vascular

Alzheimer’s disease (AD) is an incurable neurodegenerative disease and the most common form of dementia, and AD and type II diabetes (DM II) are two of the most common diseases of aging. Numerous studies demonstrate DM II with increased risk for dementia, however, the mechanisms linking DM II and AD have not been fully elucidated. There is increasing evidence suggesting that cerebral vascular dysfunction plays an important role in the development of AD. T2DN rat is a DM II rat model that exhibits diabetic nephropathy. The present study examines whether aged T2DN rat is associated with cognitive impairment, and whether autoregulation of cerebral blood flow (CBF) is impaired that contributes to AD. The levels of glucose (422 ± 32 vs. 94 ± 3 mg/dL) and glycated hemoglobin (HbA 1c , 11.5 ± 0.2 vs. 4.3 ± 0.1%) were higher in 12-18 months old T2DN than in age matched SD control rats. CBF rose by 137 ± 15% and 36 ± 5%, respectively, in T2DN and SD rats when MAP was increased from 100 to 180 mmHg. Aged T2DN rats exhibited BBB leakage and “AD” like cerebral vascular remodeling. The expression of Amyloid β 42 (Aβ 4 2 ), p-tau (S416), GFAP and IL-1 beta were significantly higher in the brains of T2DN vs. SD rats. T2DN rats also exhibited learning and memory dysfunction as the short term (2-hour; T2DN 96 ± 12 vs. SD 13 ± 3 seconds) and long term (24-hour; T2DN 105 ± 15 vs. SD 8 ± 2 seconds) latency of escape were longer in an eight-arm water maze test, and spent less time in the target arm 48 hours after training (T2DN 3.4 ± 2.6 vs. SD 45.0 ± 1.7%). These findings indicate that T2DN is a new type II diabetic rat model. Elderly T2DN rat is associated with an impaired autoregulation of CBF, glial activation and inflammation which may contribute to the development of cognitive impairment and AD.

scholarly journals EFEK ANTIDIABETIK EKSTRAK ETANOL DAUN MAHKOTA DEWA (Phaleria macrocarpa) PADA TIKUS DIABETES YANG DIINDUKSI STREPTOZOTOSIN

Biomedika ◽  
2018 ◽  
Vol 10 (2) ◽  
Author(s):  
Ira Cinta Lestari
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Rat Model ◽  
Ethanolic Extract ◽  
Diabetic Rat ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Phaleria Macrocarpa ◽  
Diabetic Rat Model

ABSTRAKDaun mahkota dewa (Phaleria macrocarpa) diketahui memiliki efek anti hiperglikemik dengan menghambat aktivitas enzim pencerna karbohidrat a-glucosidase, namun efeknya pada kondisi diabetes belum diketahui. Penelitian ini bertujuan untuk mengetahui efek antidiabetik ekstrak etanol daun mahkota dewa (EEDMD) terhadap berat badan dan kadar glukosa darah tikus model diabetes. Studi eksperimental dengan rancangan post test only control group design dilakukan terhadap subjek 45 ekor tikus Sprague Dawley. Subjek dikelompokkan dalam kontrol normal, kontrol diabetes diberi pelarut dan diabetes diberi 7mg/200g, 14mg/200g, and 28mg/200g  EEDMD secara peroral, sekali sehari selama 3, 14 dan 25 hari. Model tikus diabetes dibuat dengan injeksi streptozotosin dan nicotinamide. Hasil analisa statistik berat badan dan kadar glukosa puasa antar kelompok terdapat perbedaan yang signifikan. Sehingga kesimpulan penelitian ini adalah pemberian EEDMD memiliki efek antidiabetik pada tikus diabetes yang dinduksi stretozotosin.Kata kunci: Diabetes Mellitus, Phaleria Macrocarpa, Ekstrak Etanol, Streptozotosin, Antidiabetik ABSTRACTPhaleria macrocarpa leaf has been known to have anti-hyperglycemic effects by inhibiting the activity of a-glucosidase carbohydrates digestive enzyme, but the systemic effect on diabetic condition is unknown yet. This study was conducted to investigate the antidiabetic effect of ethanolic extract of Phaleria macrocarpa leaf (EEPML) on body weight and blood glucose levels of diabetic rat model. This was a quasi experimental study with post test only control group design. Fourty five male Sprague Dawley rats were classified into normal control group, diabetic control group with solvent, diabetic with 7mg/200g, 14mg/200g, and 28mg/200g of EEPML peroral administration, once a day for 3, 14 and 25 days. The diabetic rat model was made by streptozotocin and nicotinamide injection. Results : Statistical analysis of mean body weight and fasting blood glucose level showed there were significant differences between treatment groups. Conclusion : Administration of EEPML is able to affect the body weight and blood glucose level of diabetic rat model. Keywords: Diabetes Mellitus, Phaleria Macrocarpa, Ethanolic Extract, Streptozotocin, Antidiabetic

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