Europium Complexes as Novel Indicators of Paracellular Diffusion

2012 ◽  
Vol 9 (9) ◽  
pp. 1916-1922 ◽  
Author(s):  
Zhihan Xu ◽  
Chengyue Zhang ◽  
Yue Zhang ◽  
Xiaoda Yang
2018 ◽  
Vol 57 (13) ◽  
pp. 7512-7515 ◽  
Author(s):  
Chen Wei ◽  
Boxun Sun ◽  
Zelun Cai ◽  
Zifeng Zhao ◽  
Yu Tan ◽  
...  

1992 ◽  
Vol 99 (2) ◽  
pp. 241-262 ◽  
Author(s):  
G A Altenberg ◽  
J S Stoddard ◽  
L Reuss

In Necturus gallbladder epithelium, lowering serosal [Na+] ([Na+]s) reversibly hyperpolarized the basolateral cell membrane voltage (Vcs) and reduced the fractional resistance of the apical membrane (fRa). Previous results have suggested that there is no sizable basolateral Na+ conductance and that there are apical Ca(2+)-activated K+ channels. Here, we studied the mechanisms of the electrophysiological effects of lowering [Na+]s, in particular the possibility that an elevation in intracellular free [Ca2+] hyperpolarizes Vcs by increasing gK+. When [Na+]s was reduced from 100.5 to 10.5 mM (tetramethylammonium substitution), Vcs hyperpolarized from -68 +/- 2 to a peak value of -82 +/- 2 mV (P less than 0.001), and fRa decreased from 0.84 +/- 0.02 to 0.62 +/- 0.02 (P less than 0.001). Addition of 5 mM tetraethylammonium (TEA+) to the mucosal solution reduced both the hyperpolarization of Vcs and the change in fRa, whereas serosal addition of TEA+ had no effect. Ouabain (10(-4) M, serosal side) produced a small depolarization of Vcs and reduced the hyperpolarization upon lowering [Na+]s, without affecting the decrease in fRa. The effects of mucosal TEA+ and serosal ouabain were additive. Neither amiloride (10(-5) or 10(-3) M) nor tetrodotoxin (10(-6) M) had any effects on Vcs or fRa or on their responses to lowering [Na+]s, suggesting that basolateral Na+ channels do not contribute to the control membrane voltage or to the hyperpolarization upon lowering [Na+]s. The basolateral membrane depolarization upon elevating [K+]s was increased transiently during the hyperpolarization of Vcs upon lowering [Na+]s. Since cable analysis experiments show that basolateral membrane resistance increased, a decrease in basolateral Cl- conductance (gCl-) is the main cause of the increased K+ selectivity. Lowering [Na+]s increases intracellular free [Ca2+], which may be responsible for the increase in the apical membrane TEA(+)-sensitive gK+. We conclude that the decrease in fRa by lowering [Na+]s is mainly caused by an increase in intracellular free [Ca2+], which activates TEA(+)-sensitive maxi K+ channels at the apical membrane and decreases apical membrane resistance. The hyperpolarization of Vcs is due to increase in: (a) apical membrane gK+, (b) the contribution of the Na+ pump to Vcs, (c) basolateral membrane K+ selectivity (decreased gCl-), and (d) intraepithelial current flow brought about by a paracellular diffusion potential.


1983 ◽  
Vol 93 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Gin-Ya Adachi ◽  
Kenji Sorita ◽  
Kenji Kawata ◽  
Katsuhiko Tomokiyo ◽  
Jiro Shiokawa

2008 ◽  
Author(s):  
Lori K. Chinen ◽  
Karen P. Galen ◽  
K. T. Kuan ◽  
Mary E. Dyszlewski ◽  
Hiroaki Ozaki ◽  
...  

2017 ◽  
Vol 41 (18) ◽  
pp. 9826-9839 ◽  
Author(s):  
Boddula Rajamouli ◽  
Rachna Devi ◽  
Abhijeet Mohanty ◽  
Venkata Krishnan ◽  
Sivakumar Vaidyanathan

The red light emitting diode (LED) was fabricated by using europium complexes with InGaN LED (395 nm) and shown digital images, corresponding CIE color coordinates (red region) as well as obtained highest quantum yield of the thin film (78.7%).


2014 ◽  
Vol 14 (6) ◽  
pp. 4357-4362 ◽  
Author(s):  
Hongyan Zhao ◽  
Feng Song ◽  
Fengxiao Wang ◽  
Jiadong Liu ◽  
Yanling Liu ◽  
...  

2013 ◽  
Vol 31 (10) ◽  
pp. 950-956 ◽  
Author(s):  
Meijuan LIN ◽  
Xiaoping WANG ◽  
Qiang TANG ◽  
Qidan LING

2009 ◽  
Vol 159 (5-6) ◽  
pp. 528-536 ◽  
Author(s):  
Clémentine Féau ◽  
Emmanuel Klein ◽  
Paul Kerth ◽  
Luc Lebeau
Keyword(s):  

Sign in / Sign up

Export Citation Format

Share Document