Specific DNA Duplex Formation at an Artificial Lipid Bilayer: towards a New DNA Biosensor Technology

2012 ◽  
Vol 9 (2) ◽  
pp. 272-281 ◽  
Author(s):  
Emma Werz ◽  
Sergei Korneev ◽  
Malayko Montilla-Martinez ◽  
Richard Wagner ◽  
Roland Hemmler ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Dna Biosensor ◽  
Dna Duplex ◽  
Biosensor Technology ◽  
Duplex Formation

Successive Fusion of Vesicles Aggregated by DNA Duplex Formation in the Presence of Triton X-100

2008 ◽  
Vol 37 (3) ◽  
pp. 340-341 ◽  
Author(s):  
Naoto Maru ◽  
Koh-ichiroh Shohda ◽  
Tadashi Sugawara
Keyword(s):  
Dna Duplex ◽  
Triton X ◽  
Duplex Formation

Direct Visualization of DNA Duplex Formation on the Surface of a Giant Liposome

ChemBioChem ◽  
2003 ◽  
Vol 4 (8) ◽  
pp. 778-781 ◽  
Author(s):  
Koh-ichiroh Shohda ◽  
Taro Toyota ◽  
Tetsuya Yomo ◽  
Tadashi Sugawara
Keyword(s):  
Direct Visualization ◽  
Dna Duplex ◽  
Duplex Formation ◽  
Giant Liposome

scholarly journals Specific DNA duplex formation at an artificial lipid bilayer: fluorescence microscopy after Sybr Green I staining

2014 ◽  
Vol 10 ◽  
pp. 2307-2321 ◽  
Author(s):  
Emma Werz ◽  
Helmut Rosemeyer
Keyword(s):  
Lipid Bilayer ◽  
Single Molecule ◽  
Dna Sequences ◽  
Sybr Green ◽  
Sybr Green I ◽  
Racemic Mixture ◽  
Dna Duplexes ◽  
Target Dna ◽  
Single Molecule Fluorescence Spectroscopy ◽  
Duplex Formation

The article describes the immobilization of different probe oligonucleotides (4, 7, 10) carrying each a racemic mixture of 2,3-bis(hexadecyloxy)propan-1-ol (1a) at the 5’-terminus on a stable artificial lipid bilayer composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). The bilayer separates two compartments (cis/trans channel) of an optical transparent microfluidic sample carrier with perfusion capabilities. Injection of unlabeled target DNA sequences (6, 8, or 9), differing in sequence and length, leads in the case of complementarity to the formation of stable DNA duplexes at the bilayer surface. This could be verified by Sybr Green I double strand staining, followed by incubation periods and thorough perfusions, and was visualized by single molecule fluorescence spectroscopy and microscopy. The different bilayer-immobilized complexes consisting of various DNA duplexes and the fluorescent dye were studied with respect to the kinetics of their formation as well as to their stability against perfusion.

Photocontrol of DNA Duplex Formation by Using Azobenzene-Bearing Oligonucleotides

ChemBioChem ◽  
2001 ◽  
Vol 2 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Hiroyuki Asanuma ◽  
Xingguo Liang ◽  
Takayuki Yoshida ◽  
Makoto Komiyama
Keyword(s):  
Dna Duplex ◽  
Duplex Formation

scholarly journals Dynamics of the DNA Duplex Formation Studied by Single Molecule Force Measurements

2004 ◽  
Vol 87 (5) ◽  
pp. 3388-3396 ◽  
Author(s):  
U. Bockelmann ◽  
P. Thomen ◽  
F. Heslot
Keyword(s):  
Single Molecule ◽  
Force Measurements ◽  
Dna Duplex ◽  
Duplex Formation

Vacuum-ultraviolet circular dichroism reveals DNA duplex formation between short strands of adenine and thymine

2012 ◽  
Vol 14 (43) ◽  
pp. 15054 ◽  
Author(s):  
Lisbeth Munksgaard Nielsen ◽  
Søren Vrønning Hoffmann ◽  
Steen Brøndsted Nielsen
Keyword(s):  
Circular Dichroism ◽  
Vacuum Ultraviolet ◽  
Dna Duplex ◽  
Duplex Formation ◽  
Circular Dichroïsm
Sign in / Sign up

Export Citation Format

Share Document