Metabolism of Methoxychlor by the P450-Monooxygenase CYP6G1 Involved in Insecticide Resistance ofDrosophila melanogasterafter Expression in Cell Cultures ofNicotiana tabacum

2010 ◽  
Vol 7 (3) ◽  
pp. 722-735 ◽  
Author(s):  
Nicole Joußen ◽  
Ingolf Schuphan ◽  
Burkhard Schmidt
Keyword(s):  
Insecticide Resistance ◽  
Cell Cultures ◽  
P450 Monooxygenase ◽  
Ofnicotiana Tabacum

Deoxypodophyllotoxin 6-hydroxylase, a cytochrome P450 monooxygenase from cell cultures of Linum flavum involved in the biosynthesis of cytotoxic lignans

Planta ◽  
2001 ◽  
Vol 214 (2) ◽  
pp. 288-294 ◽  
Author(s):  
Grietje Molog ◽  
Ursula Empt ◽  
Stephan Kuhlmann ◽  
Wim van Uden ◽  
Niesko Pras ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cell Cultures ◽  
Cytochrome P450 Monooxygenase ◽  
P450 Monooxygenase ◽  
Linum Flavum

Insecticide Resistance, and Its Effects on Bait Performance in Field-Collected German Cockroaches (Blattodea: Ectobiidae) From Taiwan

2020 ◽  
Vol 113 (3) ◽  
pp. 1389-1398
Author(s):  
I-Hsuan Hu ◽  
Shan-Min Chen ◽  
Chow-Yang Lee ◽  
Kok-Boon Neoh
Keyword(s):  
Cytochrome P450 ◽  
Insecticide Resistance ◽  
Cytochrome P450 Monooxygenase ◽  
Contact Method ◽  
P450 Monooxygenase ◽  
Deltamethrin Resistance ◽  
Two Generations ◽  
Taiwan Island ◽  
Cockroach Blattella Germanica ◽  
Very High

Abstract Insecticide resistance in the German cockroach, Blattella germanica (L.), is a significant challenge to the pest management professionals worldwide. We collected 24 field populations of B. germanica from different localities in Taiwan island, reared them for one to two generations, and evaluated them for their resistance to deltamethrin, propoxur, and fipronil using the surface-contact method. Results showed that deltamethrin resistance ratio ranged from 1.5 to 817.5×. Among the strains, TC Supermarket, TC Sanshang Logistics, TC THSR, and TC 1Taichungsteak strains showed very high resistance to deltamethrin, which mortality ranged between 0 and 33% at 7-d post-treatment. On the other hand, resistance to propoxur and fipronil RR were 0.70–7.13× and 1.67–3.72×, respectively. Synergism studies using piperonyl butoxide (PBO) and S,S,S-tributylphosphorotrithioate (DEF) suggested the major involvement of cytochrome P450 monooxygenase and minor involvement of esterases. However, deltamethrin resistance in two strains (i.e., TC Supermarket and TC THSR) was not affected by both PBO and DEF, indicating that other mechanisms are involved in the resistance, including kdr resistance. Evaluation of the field strains using commercial gel baits containing fipronil, imidacloprid, hydramethylnon, and indoxacarb for up to 7 d resulted in 24.4–100%, 11.3–78.5%, 15.8–75.5%, and 63.3–100% mortality, respectively. We found that high deltamethrin resistance in some strains could affect the performance of fipronil, imidacloprid, and indoxacarb baits, indicating the potential involvement of cytochrome P450 monooxygenase in reducing the effectiveness of the bait toxicants.

Justicidin B 7-hydroxylase, a cytochrome P450 monooxygenase from cell cultures of Linum perenne Himmelszelt involved in the biosynthesis of diphyllin

2007 ◽  
Vol 68 (22-24) ◽  
pp. 2736-2743 ◽  
Author(s):  
Shiva Hemmati ◽  
Bernd Schneider ◽  
Thomas J. Schmidt ◽  
Katja Federolf ◽  
A. Wilhelm Alfermann ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cell Cultures ◽  
Cytochrome P450 Monooxygenase ◽  
P450 Monooxygenase

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Bacteriophages Associated with Turkey Coecums in Bluecomb-Infected and Healthy Birds

1969 ◽  
Vol 27 ◽  
pp. 226-227
Author(s):  
A. E. Ritchie
Keyword(s):  
Host Cell ◽  
Causal Relationship ◽  
Cell Cultures ◽  
Cell Interaction ◽  
Etiologic Agent ◽  
Viral Origin ◽  
Intestinal Contents ◽  
Host Cell Interaction

The cause of bluecomb disease in turkeys is unknown. Filtration of infective intestinal contents suggests a viral origin. To date, it has not been possible to isolate the etiologic agent in various cell cultures. The purpose of this work was to characterize as many virus-like entities as were recognizable in intestines of both healthy and bluecomb-infected turkeys. By a comparison of the viral populations it was hoped that some insight might be gained into the cause of this disease. Studies of turkey hemorraghic enteritis by Gross and Moore (Avian Dis. 11: 296-307, 1967) have suggested that a bacteriophage-host cell interaction may bear some causal relationship to that disease.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

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