scholarly journals The B subunit ofEscherichia colienterotoxin helps control the in vivo growth of solid tumors expressing the Epstein-Barr virus latent membrane protein 2A

2015 ◽  
Vol 4 (3) ◽  
pp. 457-471
Author(s):  
Beatrice Ondondo ◽  
Lee Faulkner ◽  
Neil A. Williams ◽  
Andrew J. Morgan ◽  
David J. Morgan
Keyword(s):  
Membrane Protein ◽  
Epstein Barr Virus ◽  
Latent Membrane Protein ◽  
Latent Membrane Protein 2A ◽  
Barr Virus ◽  
B Subunit ◽  
Epstein Barr ◽  
In Vivo Growth ◽  
Virus Latent Membrane Protein

scholarly journals Roles of the ITAM and PY Motifs of Epstein-Barr Virus Latent Membrane Protein 2A in the Inhibition of Epithelial Cell Differentiation and Activation of β-Catenin Signaling

2005 ◽  
Vol 79 (4) ◽  
pp. 2375-2382 ◽  
Author(s):  
Jennifer A. Morrison ◽  
Nancy Raab-Traub
Keyword(s):  
Membrane Protein ◽  
Epstein Barr Virus ◽  
Nuclear Translocation ◽  
Cell Receptor ◽  
Latent Membrane Protein ◽  
Latent Membrane Protein 2A ◽  
Barr Virus ◽  
Cellular Processes ◽  
Epstein Barr ◽  
Virus Latent Membrane Protein

ABSTRACT Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) is important for maintenance of latency in infected B lymphocytes. Through its immunoreceptor tyrosine-based activation motif (ITAM) and PY motifs, LMP2A is able to block B-cell receptor (BCR) signaling, bind BCR-associated kinases, and manipulate the turnover of itself and these kinases via a PY-mediated interaction with the Nedd4 family of ubiquitin ligases. In epithelial cells, LMP2A has been shown to activate the phosphatidylinositol 3′-OH kinase/Akt and β-catenin signaling pathways. In the present study, the biological consequences of LMP2A expression in the normal human foreskin keratinocyte (HFK) cell line were investigated and the importance of the ITAM and PY motifs for LMP2A signaling effects in HFK cells was ascertained. The ITAM was essential for the activation of Akt by LMP2A in HFK cells, while both the ITAM and PY motifs contributed to LMP2A-mediated accumulation and nuclear translocation of the oncoprotein β-catenin. LMP2A inhibited induction of differentiation in an assay conducted with semisolid methylcellulose medium, and the PY motifs were critical for this inhibition. LMP2A is expressed in the EBV-associated epithelial malignancies nasopharyngeal carcinoma and gastric carcinoma, and these data indicate that LMP2A affects cellular processes that likely contribute to carcinogenesis.

scholarly journals Role of Epstein–Barr virus-encoded latent membrane protein 2A on virus-induced immortalization and virus activation

2001 ◽  
Vol 82 (6) ◽  
pp. 1451-1456 ◽  
Author(s):  
Kazuya Konishi ◽  
Seiji Maruo ◽  
Hiroyuki Kato ◽  
Kenzo Takada
Keyword(s):  
Membrane Protein ◽  
B Lymphocytes ◽  
Virus Replication ◽  
Epstein Barr Virus ◽  
Latent Membrane Protein ◽  
Latent Membrane Protein 2A ◽  
Barr Virus ◽  
Epstein Barr

To quantitatively evaluate the role of Epstein-Barr virus (EBV)-encoded latent membrane protein 2A (LMP2A) in immortalization of peripheral B-lymphocytes, we used the Akata cell system to generate an EBV recombinant in which the first exon of the LMP2A gene was disrupted. The results indicated that deletion of the LMP2A gene did not affect the immortalization efficiency of EBV in B-lymphocytes. Deletion of the LMP2A gene made EBV-transformed lymphocytes more permissive for virus replication in response to surface immunoglobulin cross-linking. On the other hand Akata cells, in which LMP2A expression was much lower than in EBV-transformed lymphocytes, were equally permissive for virus replication whether they were infected with wild EBV or LMP2A-knockout EBV. The results raise a question as to the role of LMP2A in inhibition of disruption of virus latency in vivo, where LMP2A expression has been expected to be low as in Akata cells.

scholarly journals In vivo growth of Epstein-Barr virus transformed B cells with mutations in latent membrane protein 2 (LMP2)

1997 ◽  
Vol 142 (4) ◽  
pp. 707-720 ◽  
Author(s):  
R. Rochford ◽  
C. L. Miller ◽  
M. J. Cannon ◽  
K. M. Izumi ◽  
E. Kieff ◽  
...  
Keyword(s):  
B Cells ◽  
Membrane Protein ◽  
Epstein Barr Virus ◽  
Latent Membrane Protein ◽  
Barr Virus ◽  
Epstein Barr ◽  
In Vivo Growth ◽  
Latent Membrane Protein 2
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