scholarly journals Stromal score as a prognostic factor in primary gastric cancer and close association with tumor immune microenvironment

2020 ◽  
Vol 9 (14) ◽  
pp. 4980-4990
Author(s):  
Min Mao ◽  
Qingliang Yu ◽  
Rongzhi Huang ◽  
Yunxin Lu ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Factor ◽  
Close Association ◽  
Immune Microenvironment ◽  
Primary Gastric Cancer ◽  
Tumor Immune Microenvironment

scholarly journals New insights into the inflamed tumor immune microenvironment of gastric cancer with lymphoid stroma: from morphology and digital analysis to gene expression

2018 ◽  
Vol 22 (1) ◽  
pp. 77-90 ◽  
Author(s):  
Irene Gullo ◽  
Patrícia Oliveira ◽  
Maria Athelogou ◽  
Gilza Gonçalves ◽  
Marta L. Pinto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Digital Analysis ◽  
Immune Microenvironment ◽  
Lymphoid Stroma ◽  
Tumor Immune Microenvironment

scholarly journals Prognostic significance of tumor immune microenvironment and immunotherapy: Novel insights and future perspectives in gastric cancer

2018 ◽  
Vol 24 (32) ◽  
pp. 3583-3616 ◽  
Author(s):  
Daniela Cornelia Lazăr ◽  
Mihaela Flavia Avram ◽  
Ioan Romoșan ◽  
Mărioara Cornianu ◽  
Sorina Tăban ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Significance ◽  
Future Perspectives ◽  
Immune Microenvironment ◽  
Tumor Immune Microenvironment

scholarly journals CHARACTERISTICS OF THE IMMUNE MICROENVIRONMENT OF THE NORMAL MUCOUS MEMBRANE OF THE PERITUMORAL AREA IS AN ADDITIONAL INDEPENDENT PROGNOSTIC FACTOR IN GASTRIC CANCER

2021 ◽  
Vol 20 (1) ◽  
pp. 74-86
Author(s):  
N. V. Danilova ◽  
V. M. Kkomyakov ◽  
A. V. Chayka ◽  
I. A. Mikhailov ◽  
N. A. Oleynikova ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Factor ◽  
Mucous Membrane ◽  
Patient Survival ◽  
Lymphocyte Infiltration ◽  
Immune Microenvironment ◽  
Average Area ◽  
Negative Prognostic Factor ◽  
Normal Mucous Membrane ◽  
Analysis System

The aim of the study was to study and evaluate the predictive value of the immune microenvironment of gastric cancer and morphologically normal mucous membrane of the peritumoral area using an automatic morphometric analysis system on the example of CD 8+ cells.Material and Methods. Surgical samples from 130 patients with a verified diagnosis of gastric cancer were used. After immunohistochemical staining with antibodies to CD 8, a morphological assessment was performed according to the original method. We assessed the average area of CD 8+ cells in three fields of view (lens magn. ×20) using the automatic system of morphometric analysis LAS X (Leica) in the central part of the tumor and areas of morphologically normal mucous membrane of the peritumoral region directly adjacent to the tumor tissue. The results were compared with the main clinical and morphological characteristics of the tumor as well as with the overall five-year survival of patients.Results and Discussion. A high density of CD 8+ infiltration of normal mucous membrane of the peritumoral area was observed in groups T4a and T4b by the depth of invasion (n=96, p=0.0089) and was associated with the presence of emboli in the lymphatic vessels (n=96, p=0.0102) and with the more advanced stage of gastric cancer (n=96, p=0.0107). The studied cases were divided into two groups: less than 3300 square micrometers (better patient survival; n=79, p=0.01) and more than 3300 square micrometers according to the average area of CD 8+ cells in normal mucous membrane of the peritumoral area. According to multivariate survival analysis using the Cox regression model, it was found that the average area of CD 8+ cells in normal mucous membrane of the peritumoral area was a significant negative prognostic factor (RR=1.537; CI : 0.761–3.105; p<0.01) comparable in degree covariance with the stage of the tumor A similar indicator assessed in central part of the tumor was not significantly associated with patient survival (RR=0.803; CI : 0.574–1.122; p>0.05).Conclusion. The possibility of using an automatic analysis system to evaluate the immune microenvironment in gastric cancer was demonstrated for the first time. It was found that a high level of CD 8+ lymphocyte infiltration of morphologically normal mucous membrane of the peritumoral area was an independent negative prognostic factor. Therefore, we recommend the mandatory preoperative biopsy sampling from the mucous membrane of the peritumoral region for morphometric assessment of CD 8+ lymphocyte infiltration. 

Determination of hypoxia signature to predict prognosis and the tumor immune microenvironment in melanoma

2021 ◽  
Vol 17 (2) ◽  
pp. 307-316
Author(s):  
Yanhong Shou ◽  
Lu Yang ◽  
Yongsheng Yang ◽  
Xiaohua Zhu ◽  
Feng Li ◽  
...  
Keyword(s):  
Prognostic Factor ◽  
Predictive Biomarker ◽  
Independent Prognostic Factor ◽  
Immune Microenvironment ◽  
Tumor Immune Microenvironment ◽  
Hypoxia Signature

The hypoxia score is identified as an independent prognostic factor and a predictive biomarker of the immune microenvironment for melanoma.

Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined by methylation analyses and its prognostic significance in gastric cancer.

2018 ◽  
Vol 36 (4_suppl) ◽  
pp. 66-66
Author(s):  
Koung Jin Suh ◽  
Jin Won Kim ◽  
Ji Eun Kim ◽  
Jiwon Koh ◽  
Ji-Won Kim ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
T Cell ◽  
Tumor Microenvironment ◽  
Cancer Patients ◽  
Immune Microenvironment ◽  
Cell Ratio ◽  
Cell Counts ◽  
Potential Biomarker ◽  
Tumor Immune Microenvironment ◽  
Gastric Cancer Patients

66 Background: Peripheral regulatory T cells (pTregs) might involve in tumor immune microenvironment. We aim to evaluate the correlation between pTregs and tumor immune microenvironment. Methods: pTregs status was determined from assessment of the pTreg/total T cell ratio (ratio of Foxp3 Treg-specific demethylated region (TSDR) to CD3G/CD3D demethylation) through epigenetic pattern of bisulfite pyrosequencing in long-term stored peripheral blood of 442 gastric cancer patients who received curative surgery. Immunohistochemical staining of multiple immune-related markers including CD3, CD4, CD8, Foxp3, 1-selectin arginase, ADAM metallopeptidase domain 17, CD163 and CD45RO within tumor microenvironment were performed in resected gastric tumor specimen. Results: The median value of FoxP3-TSDR and CD3G/CD3D demethylation was 5.8% and 32.3%, respectively. When pTreg/total T cell ratio was divided into eight equal groups from the lowest to the highest value, the extreme pTreg/total T cell ratio of the upper eighth and the lower eighth was significantly associated with lower CD45RO expressed cell counts within tumor microenvironment. In terms of arginase and CD163, inverse results were observed. Patients with extreme pTreg/total T cell ratio had significantly shorter disease-free survival (DFS) and overall survival (OS) compared to the patients with non-extreme pTreg/total T cell ratio. Multivariate analysis which included age, stage, lymphatic invasion, vascular invasion, and perineural invasion as covariates demonstrated that the extreme pTreg/total T cell ratio was an independent predictor for shorter DFS (HR 1.740, 95% CI 1.128 – 2.682; p = 0.012) and OS (HR 1.900, 95% CI 1.175 – 3.070; p = 0.003). Conclusions: Our results suggest that the pTreg/total T cell ratio determined by epigenetic methylation analysis is correlated with specific immune cell infiltration within tumor microenvironment in resected gastric tumors, and gives prognostic information in gastric cancer patients. pTreg/total T cell ratio could be an easy-obtained potential biomarker for prognosis and future immunotherapeutic treatment strategies.

scholarly journals Screening and Validation of the Hypoxia-Related Signature of Evaluating Tumor Immune Microenvironment and Predicting Prognosis in Gastric Cancer

2021 ◽  
Vol 12 ◽  
Author(s):  
Jun-Peng Pei ◽  
Chun-Dong Zhang ◽  
Maimaititusun Yusupu ◽  
Cheng Zhang ◽  
Dong-Qiu Dai
Keyword(s):  
Gastric Cancer ◽  
Risk Score ◽  
Poor Prognosis ◽  
Immune Escape ◽  
The Cancer Genome Atlas ◽  
Signal Pathways ◽  
Immune Microenvironment ◽  
Good Ability ◽  
Tumor Immune Microenvironment ◽  
Score Model

BackgroundHypoxia is one driving factor of gastric cancer. It causes a series of immunosuppressive processes and malignant cell responses, leading to a poor prognosis. It is clinically important to identify the molecular markers related to hypoxia.MethodsWe screened the prognostic markers related to hypoxia in The Cancer Genome Atlas database, and a risk score model was developed based on these markers. The relationships between the risk score and tumor immune microenvironment were investigated. An independent validation cohort from Gene Expression Omnibus was applied to validate the results. A nomogram of risk score model and clinicopathological factor was developed to individually predict the prognosis.ResultsWe developed a hypoxia risk score model based on SERPINE1 and EFNA3. Quantified real-time PCR was further applied to verified gene expressions of SERPINE1 and EFNA3 in gastric cancer patients and cell lines. A high-risk score is associated with a poor prognosis through the immunosuppressive microenvironment and immune escape mechanisms, including infiltration of immunosuppressive cells, expression of immune checkpoint molecules, and enrichment of signal pathways related to cancer and immunosuppression. The nomogram basing on the hypoxia-related risk score model showed a good ability to predict prognosis and high clinical net benefits.ConclusionsThe hypoxia risk score model revealed a close relationship between hypoxia and tumor immune microenvironment. The current study potentially provides new insights of how hypoxia affects the prognosis, and may provide a new therapeutic target for patients with gastric cancer.

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