scholarly journals Circular RNA expression profiles of peripheral blood mononuclear cells in hepatocellular carcinoma patients by sequence analysis

2019 ◽  
Vol 8 (4) ◽  
pp. 1423-1433 ◽  
Author(s):  
Bo Lei ◽  
Jian Zhou ◽  
Xiuyun Xuan ◽  
Zhiqiang Tian ◽  
Mengjie Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Sequence Analysis ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Expression Profiles ◽  
Circular Rna ◽  
Rna Expression ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Identification of BHLHE40 expression in peripheral blood mononuclear cells as a novel biomarker for diagnosis and prognosis of hepatocellular carcinoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pattapon Kunadirek ◽  
Chaiyaboot Ariyachet ◽  
Supachaya Sriphoosanaphan ◽  
Nutcha Pinjaroen ◽  
Pongserath Sirichindakul ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Early Stage ◽  
High Sensitivity ◽  
Peripheral Blood Mononuclear ◽  
Diagnosis And Prognosis ◽  
Transcription Profiles ◽  
Blood Mononuclear Cells

AbstractNovel and sensitive biomarkers is highly required for early detection and predicting prognosis of hepatocellular carcinoma (HCC). Here, we investigated transcription profiles from peripheral blood mononuclear cells (PBMCs) of 8 patients with HCC and PBMCs from co-culture model with HCC using RNA-Sequencing. These transcription profiles were cross compared with published microarray datasets of PBMCs in HCC to identify differentially expressed genes (DEGs). A total of commonly identified of 24 DEGs among these data were proposed as cancer-induced genes in PBMCs, including 18 upregulated and 6 downregulated DEGs. The KEGG pathway showed that these enriched genes were mainly associated with immune responses. Five up-regulated candidate genes including BHLHE40, AREG, SOCS1, CCL5, and DDIT4 were selected and further validated in PBMCs of 100 patients with HBV-related HCC, 100 patients with chronic HBV infection and 100 healthy controls. Based on ROC analysis, BHLHE40 and DDIT4 displayed better diagnostic performance than alpha-fetoprotein (AFP) in discriminating HCC from controls. Additionally, BHLHE40 and DDIT4 had high sensitivity for detecting AFP-negative and early-stage HCC. BHLHE40 was also emerged as an independent prognostic factor of overall survival of HCC. Together, our study indicated that BHLHE40 in PBMCs could be a promising diagnostic and prognostic biomarker for HBV-related HCC.

scholarly journals The circular RNA landscape in specific peripheral blood mononuclear cells of critically ill patients with sepsis

Critical Care ◽  
2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Hina N. Khan ◽  
Xanthe Brands ◽  
Simona Aufiero ◽  
Arie J. Hoogendijk ◽  
Augustijn M. Klarenbeek ◽  
...  
Keyword(s):  
Critically Ill ◽  
Peripheral Blood Mononuclear Cells ◽  
Critically Ill Patients ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Circular Rna ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals SP801TOLL-LIKE RECEPTOR M-RNA EXPRESSION IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN PATIENTS WITH LONG-TERM KIDNEY ALLOGRAFT SURVIVAL

2015 ◽  
Vol 30 (suppl_3) ◽  
pp. iii642-iii642
Author(s):  
Sławomir C. Zmonarski ◽  
Katarzyna Koscielska ◽  
Madziarska Katarzyna ◽  
Myszka Marta ◽  
Magott-Procelewska Maria ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Rna Expression ◽  
Allograft Survival ◽  
Peripheral Blood Mononuclear ◽  
Kidney Allograft ◽  
Blood Mononuclear Cells

Circular RNA expression profiles and bioinformatic analysis in coronary heart disease

Epigenomics ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 439-454 ◽  
Author(s):  
Fangpu Yu ◽  
Yuanyuan Tie ◽  
Ya Zhang ◽  
Zunzhe Wang ◽  
Liwen Yu ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Coronary Arteries ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Expression Profiles ◽  
Peripheral Blood Mononuclear ◽  
Qrt Pcr ◽  
Blood Mononuclear Cells

Aim: We aimed to identify the expression profile and role of circular RNAs (circRNAs) in coronary heart disease (CHD). Materials & methods: We performed sequence analysis of circRNAs in peripheral blood mononuclear cells of 70 CHD patients and 30 controls. Eight selected circRNAs were validated using quantitative real-time polymerase chain reaction (qRT-PCR) in human atherosclerotic coronary arteries. Results: In total, 2283 downregulated and 85 upregulated circRNAs were identified in CHD. Parental genes of top 100 dysregulated-circRNAs are related to metabolism and protein modification, and 12 circRNAs might upregulate their CHD-related parental genes through miRNA sponges. Of the eight circRNAs validated in atherosclerotic coronary arteries by qRT-PCR, six were consistent with sequencing results of peripheral blood mononuclear cells. Conclusion: As potential ceRNAs, dysregulated circRNAs may be involved in CHD pathophysiology.

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