scholarly journals BTG2 is a tumor suppressor gene upregulated by p53 and PTEN in human bladder carcinoma cells

2017 ◽  
Vol 7 (1) ◽  
pp. 184-195 ◽  
Author(s):  
Ke-Hung Tsui ◽  
Kun-Chun Chiang ◽  
Yu-Hsiang Lin ◽  
Kang-Shuo Chang ◽  
Tsui-Hsia Feng ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Bladder Carcinoma ◽  
Suppressor Gene ◽  
Carcinoma Cells ◽  
Human Bladder ◽  
Bladder Carcinoma Cells

scholarly journals Maspin is a PTEN-Upregulated and p53-Upregulated Tumor Suppressor Gene and Acts as an HDAC1 Inhibitor in Human Bladder Cancer

Cancers ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 10 ◽  
Author(s):  
Yu-Hsiang Lin ◽  
Ke-Hung Tsui ◽  
Kang-Shuo Chang ◽  
Chen-Pang Hou ◽  
Tsui-Hsia Feng ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Tumor Suppressor ◽  
Bladder Carcinoma ◽  
Suppressor Gene ◽  
Carcinoma Cells ◽  
Human Bladder ◽  
Maspin Expression ◽  
Bladder Carcinoma Cells

Maspin is a member of the clade B serine protease inhibitor superfamily and exhibits diverse regulatory effects in various types of solid tumors. We compared the expressions of maspin and determined its potential biological functions and regulatory mechanisms in bladder carcinoma cells in vitro and in vivo. The results of RT-qPCR indicated that maspin expressed significantly lower levels in the bladder cancer tissues than in the paired normal tissues. The immunohistochemical assays of human bladder tissue arrays revealed similar results. Maspin-knockdown enhanced cell invasion whereas the overexpression of maspin resulted in the opposite process taking place. Knockdown of maspin also enhanced tumorigenesis in vivo and downregulated protein levels of acetyl-histone H3. Moreover, in bladder carcinoma cells, maspin modulated HDAC1 target genes, including cyclin D1, p21, MMP9, and vimentin. Treatment with MK2206, which is an Akt inhibitor, upregulated maspin expression, whereas PTEN-knockdown or PTEN activity inhibitor (VO-OHpic) treatments demonstrated reverse results. The ectopic overexpression of p53 or camptothecin treatment induced maspin expression. Our study indicated that maspin is a PTEN-upregulated and p53-upregulated gene that blocks cell growth in vitro and in vivo, and may act as an HDAC1 inhibitor in bladder carcinoma cells. We consider that maspin is a potential tumor suppressor gene in bladder cancer.

scholarly journals The retinoblastoma gene functions as a growth and tumor suppressor in human bladder carcinoma cells.

1991 ◽  
Vol 88 (12) ◽  
pp. 5257-5261 ◽  
Author(s):  
R. Takahashi ◽  
T. Hashimoto ◽  
H. J. Xu ◽  
S. X. Hu ◽  
T. Matsui ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Bladder Carcinoma ◽  
Carcinoma Cells ◽  
Retinoblastoma Gene ◽  
Human Bladder ◽  
Gene Functions ◽  
Bladder Carcinoma Cells

scholarly journals Molecular targets for the protodynamic action of cis-urocanic acid in human bladder carcinoma cells

BMC Cancer ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Emilia Peuhu ◽  
Aura Kaunisto ◽  
Jarmo K Laihia ◽  
Lasse Leino ◽  
John E Eriksson
Keyword(s):  
Bladder Carcinoma ◽  
Molecular Targets ◽  
Urocanic Acid ◽  
Carcinoma Cells ◽  
Human Bladder ◽  
Bladder Carcinoma Cells

scholarly journals Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells

2002 ◽  
Vol 1565 (1) ◽  
pp. 112-122 ◽  
Author(s):  
Do Kyung Kim ◽  
Yoshikatsu Kanai ◽  
Hye Won Choi ◽  
Sahatchai Tangtrongsup ◽  
Arthit Chairoungdua ◽  
...  
Keyword(s):  
Amino Acid ◽  
Bladder Carcinoma ◽  
Amino Acid Transporter ◽  
Carcinoma Cells ◽  
Human Bladder ◽  
System L ◽  
Acid Transporter ◽  
Bladder Carcinoma Cells

scholarly journals A novel model to identify interaction partners of the PTEN tumor suppressor gene in human bladder cancer

2007 ◽  
Vol 352 (2) ◽  
pp. 549-555 ◽  
Author(s):  
Mikael Herlevsen ◽  
Gary Oxford ◽  
Celeste Ptak ◽  
Jeffrey Shabanowitz ◽  
Donald F. Hunt ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Suppressor Gene ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Interaction Partners ◽  
Novel Model

scholarly journals Transgelin, a p53 and PTEN-Upregulated Gene, Inhibits the Cell Proliferation and Invasion of Human Bladder Carcinoma Cells in Vitro and in Vivo

2019 ◽  
Vol 20 (19) ◽  
pp. 4946 ◽  
Author(s):  
Tsui ◽  
Lin ◽  
Chang ◽  
Hou ◽  
Chen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Bladder Carcinoma ◽  
Carcinoma Cells ◽  
Human Bladder ◽  
Ectopic Overexpression ◽  
Proliferation And Invasion ◽  
Bladder Carcinoma Cells

Transgelin (TAGLN/SM22-α) is a regulator of the actin cytoskeleton, affecting the survival, migration, and apoptosis of various cancer cells divergently; however, the roles of TAGLN in bladder carcinoma cells remain inconclusive. We compared expressions of TAGLN in human bladder carcinoma cells to the normal human bladder tissues to determine the potential biological functions and regulatory mechanisms of TAGLN in bladder carcinoma cells. Results of RT-qPCR and immunoblot assays indicated that TAGLN expressions were higher in bladder smooth muscle cells, fibroblast cells, and normal epithelial cells than in carcinoma cells (RT-4, HT1376, TSGH-8301, and T24) in vitro. Besides, the results of RT-qPCR revealed that TAGLN expressions were higher in normal tissues than the paired tumor tissues. In vitro, TAGLN knockdown enhanced cell proliferation and invasion, while overexpression of TAGLN had the inverse effects in bladder carcinoma cells. Meanwhile, ectopic overexpression of TAGLN attenuated tumorigenesis in vivo. Immunofluorescence and immunoblot assays showed that TAGLN was predominantly in the cytosol and colocalized with F-actin. Ectopic overexpression of either p53 or PTEN induced TAGLN expression, while p53 knockdown downregulated TAGLN expression in bladder carcinoma cells. Our results indicate that TAGLN is a p53 and PTEN-upregulated gene, expressing higher levels in normal bladder epithelial cells than carcinoma cells. Further, TAGLN inhibited cell proliferation and invasion in vitro and blocked tumorigenesis in vivo. Collectively, it can be concluded that TAGLN is an antitumor gene in the human bladder.

Induction of acidic sphingomyelinase by electrophiles in human bladder carcinoma cells

2007 ◽  
Vol 149 ◽  
pp. S83
Author(s):  
Takeshi Kumagai ◽  
Yuichi Yajima ◽  
Yosuke Kozakai ◽  
Yasuhito Nakagawa
Keyword(s):  
Bladder Carcinoma ◽  
Carcinoma Cells ◽  
Human Bladder ◽  
Bladder Carcinoma Cells

Targeting the Loss of the von Hippel-Lindau Tumor Suppressor Gene in Renal Cell Carcinoma Cells

2007 ◽  
Vol 67 (12) ◽  
pp. 5896-5905 ◽  
Author(s):  
Patrick D. Sutphin ◽  
Denise A. Chan ◽  
James M. Li ◽  
Sandra Turcotte ◽  
Adam J. Krieg ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Tumor Suppressor ◽  
Cell Carcinoma ◽  
Tumor Suppressor Gene ◽  
Renal Cell ◽  
Suppressor Gene ◽  
Carcinoma Cells ◽  
Von Hippel Lindau
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