scholarly journals Analysis of spinal cord blood supply combining vascular corrosion casting and fluorescence microsphere technique: A feasibility study in an aortic surgical large animal model

2020 ◽  
Author(s):  
Babak Saravi ◽  
Karin Wittmann ◽  
Sonja Krause ◽  
Luisa Puttfarcken ◽  
Matthias Siepe ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Animal Model ◽  
Cord Blood ◽  
Feasibility Study ◽  
Blood Supply ◽  
Large Animal Model ◽  
Large Animal ◽  
Corrosion Casting ◽  
Spinal Cord Blood Supply

scholarly journals An intermediate animal model of spinal cord stimulation

2016 ◽  
Vol 26 (2) ◽  
Author(s):  
Thomas Guiho ◽  
Christine Azevedo Coste ◽  
Claire Delleci ◽  
Jean-Patrick Chenu ◽  
Jean-Rodolphe Vignes ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Animal Model ◽  
Spinal Cord Stimulation ◽  
Spinal Cord Injuries ◽  
Large Animal Model ◽  
Large Animal ◽  
Physiological Processes ◽  
Spinal Roots ◽  
And Control ◽  
Stimulation Of

Spinal cord injuries (SCI) result in the loss of movement and sensory feedback as well as organs dysfunctions. For example, nearly all SCI subjects loose their bladder control and are prone to kidney failure if they do not proceed to intermittent (self-) catheterization. Electrical stimulation of the sacral spinal roots with an implantable neuroprosthesis is a promising approach, with commercialized products, to restore continence and control micturition. However, many persons do not ask for this intervention since a surgical deafferentation is needed and the loss of sensory functions and reflexes become serious side effects of this procedure. Recent results renewed interest in spinal cord stimulation. Stimulation of existing pre-cabled neural networks involved in physiological processes regulation is suspected to enable synergic recruitment of spinal fibers. The development of direct spinal stimulation strategies aiming at bladder and bowel functions restoration would therefore appear as a credible alternative to existent solutions. However, a lack of suitable large animal model complicates these kinds of studies. In this article, we propose a new animal model of spinal stimulation -pig- and will briefly introduce results from one first acute experimental validation session.

Multi-Tissue, Long-Term Engraftment and Expansion of BCR-ABL-transduced Human Lin-CD34+ Cord Blood Cells in a Large Animal Model.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3265-3265
Author(s):  
Fanyi Zeng ◽  
Mei-Jue Chen ◽  
Zhi-Jian Gong ◽  
Don A. Baldwin ◽  
Hui Qian ◽  
...  
Keyword(s):  
Animal Model ◽  
Cord Blood ◽  
Hematopoietic Cells ◽  
In Utero ◽  
Human Cells ◽  
Laser Scanning Microscopy ◽  
Large Animal Model ◽  
Large Animal ◽  
Wbc Count

Abstract Abstract 3265 Poster Board III-1 In utero transplantation of large animals (sheep and goats) with human hematopoietic cells has proven valuable in distinguishing subsets of human cells with short- and long-term repopulating activity. Transplantation of secondary and tertiary fetal sheep with cells regenerated in primary sheep has also demonstrated the ability of human hematopoietic stem cells to retain and execute their self-renewal potential in a xenogeneic setting. We now describe the novel extension of this approach to the generation of a BCR-ABL gene transfer-based in vivo model of human myeloproliferative disease. Lin-CD34+ human cord blood (CB) cells were transduced with BCR-ABL retrovirus (MSCV-BCR-ABL-IRES-GFP) and the cells were then injected IP into pre-immune fetal goats at 45–55 days of gestation. This induced a high frequency of abortion among the injected fetuses: 79% (n=22) when >5×104BCR-ABL- transduced CB cells were injected as compared to 64% (n=9) when control (MIG)-transduced cells were injected. Six goats transplanted with 2×104 BCR-ABL-transduced cells were born alive and 3 weeks after birth, 3 of these were sacrificed so that their tissues could be analyzed. Interestingly, in the goats injected with BCR-ABL-transduced human cells, only GFP+(BCR-ABL+) human cells were detected and these were found in multiple hematopoietic and non-hematopoietic tissues, including peripheral blood, bone marrow (BM), liver, kidney, lung, heart, and skeletal and smooth muscle (1–49%) by fluorescence microscopy and confocal laser scanning microscopy, FISH and FACS. Immunohistochemical analysis allowed positive cells to also be detected in frozen sections of liver tissue. Continued follow-up of the other recipients of transduced cells showed that the 3 injected with BCR-ABL-transduced cells all developed features of early chronic phase human chronic myeloid leukemia (CML), as evidenced by a 3- to 5-fold elevation in their WBC count (up to 2.5×1010/L as compared to 5–8×109/L in the recipients of MIG-transduced cells, P<0.01). Changes in the WBC count were seen as early as 3 months after birth and up to 2.5 years post-transplant and were accompanied in all 3 of these goats by the presence of GFP+ cells, including human CD34+ stem/progenitor cells, in the blood and BM. Quantitative real-time PCR analysis of genomic DNA from multiple tissues demonstrated up to 8×104 copies of transgene per microgram of DNA. Microarray transcript profiling of blood and liver from BCR-ABL chimeric goats confirmed expression of many human genes, including 321 that were detected at >2.5-fold higher levels in the BCR-ABL chimeric goats as compared to both control chimeric goats and normal human CB. These over-expressed genes are from several functional categories, including tyrosine kinases and other proteins with phosphorylation activities, cell cycle control, cell adhesion, homing and differentiation, transcription, nucleotide binding and ion transport. Several were confirmed by quantitative RT-PCR. These results demonstrate long-term engraftment, but slow expansion of primitive human hematopoietic cells transduced with BCR-ABL fusion gene and transplanted in utero in a large animal model. This novel xenotransplant goat model should be useful for characterizing the early (pre-symptomatic) phase of human CML and for assessing new therapies with potential long-term benefits. Disclosures: No relevant conflicts of interest to declare.

Spinal Cord Blood Supply and Its Surgical Implications

2015 ◽  
Vol 23 (10) ◽  
pp. 581-591 ◽  
Author(s):  
Matthew W. Colman ◽  
Francis J. Hornicek ◽  
Joseph H. Schwab
Keyword(s):  
Spinal Cord ◽  
Cord Blood ◽  
Blood Supply ◽  
Spinal Cord Blood Supply

scholarly journals Pediatric Spinal Cord Injury in Infant Piglets: Description of a New Large Animal Model and Review of the Literature

2010 ◽  
Vol 33 (1) ◽  
pp. 43-57 ◽  
Author(s):  
John Kuluz ◽  
Amer Samdani ◽  
David Benglis ◽  
Manuel Gonzalez-Brito ◽  
Juan P. Solano ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Animal Model ◽  
Large Animal Model ◽  
Large Animal ◽  
Review Of The Literature ◽  
Cord Injury ◽  
Pediatric Spinal Cord Injury

scholarly journals Catheter-directed Intraportal Delivery of Endothelial Cell Therapy for Liver Regeneration: A Feasibility Study in a Large-Animal Model of Cirrhosis

Radiology ◽  
2017 ◽  
Vol 285 (1) ◽  
pp. 114-123 ◽  
Author(s):  
Kyungmouk Steve Lee ◽  
Sara F. Santagostino ◽  
David Li ◽  
Amit Ramjit ◽  
Kenneth Serrano ◽  
...  
Keyword(s):  
Animal Model ◽  
Endothelial Cell ◽  
Cell Therapy ◽  
Liver Regeneration ◽  
Feasibility Study ◽  
Large Animal Model ◽  
Large Animal
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