scholarly journals Expression system for recombinant human growth hormone production fromBacillus subtilis

2009 ◽  
Vol 25 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Tunçer H. Özdamar ◽  
Birgül Şentürk ◽  
Özge Deniz Yilmaz ◽  
Güzide Çalık ◽  
Eda Çelik ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Recombinant Human Growth Hormone ◽  
Expression System ◽  
Human Growth ◽  
Hormone Production

scholarly journals In Vivo Bioassay of Recombinant Human Growth Hormone Synthesized inB. moriPupae

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Hanglian Lan ◽  
Zuoming Nie ◽  
Yue Liu ◽  
Zhengbing Lv ◽  
Yingshuo Liu ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Recombinant Human Growth Hormone ◽  
Expression System ◽  
Human Growth ◽  
The Body ◽  
Control Group ◽  
Prokaryotic Expression System ◽  
Significant Difference ◽  
Baculovirus System

The human growth hormone (hGH) has been expressed in prokaryotic expression system with low bioactivity previously. Then the effectiveB. moribaculovirus system was employed to express hGH identical to mature hGH successfully in larvae, but the expression level was still limited. In this work, the hGH was expressed inB. moripupae by baculovirus system. Quantification of recombinant hGH protein (BmrhGH) showed that the expression of BmrhGH reached the level of approximately 890 μg/mL pupae supernatant solution, which was five times more than the level using larvae. Furthermore, Animals were gavaged with BmrhGH at the dose of 4.5 mg/rat.day, and the body weight gain (BWG) of treated group had a significant difference (P<.01) compared with the control group. The other two parameters of liver weight and epiphyseal width were also found to be different between the two groups (P<.05). The results suggested that BmrhGH might be used as a protein drug by oral administration.

Influence of pH on recombinant human growth hormone production by Pichia pastoris

2010 ◽  
Vol 85 (12) ◽  
pp. 1628-1635 ◽  
Author(s):  
Pınar Çalık ◽  
Eda Bayraktar ◽  
Bahar İnankur ◽  
Elif Ş. Soyaslan ◽  
Merve Şahin ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Pichia Pastoris ◽  
Human Growth Hormone ◽  
Recombinant Human Growth Hormone ◽  
Human Growth ◽  
Hormone Production ◽  
Influence Of Ph
Sign in / Sign up

Export Citation Format

Share Document