The incorporation of the A2 protein to produce novel Qβ virus-like particles using cell-free protein synthesis

2011 ◽  
Vol 28 (2) ◽  
pp. 549-555 ◽  
Author(s):  
Mark T. Smith ◽  
Chad T. Varner ◽  
Derek B. Bush ◽  
Bradley C. Bundy
Keyword(s):  
Protein Synthesis ◽  
Free Protein ◽  
Virus Like Particles ◽  
Cell Free Protein Synthesis

scholarly journals Cell-free protein synthesis of norovirus virus-like particles

RSC Advances ◽  
2017 ◽  
Vol 7 (46) ◽  
pp. 28837-28840 ◽  
Author(s):  
Jiayuan Sheng ◽  
Shaohua Lei ◽  
Lijuan Yuan ◽  
Xueyang Feng
Keyword(s):  
Protein Synthesis ◽  
Protein Expression ◽  
Free Protein ◽  
Virus Like Particles ◽  
Cell Free Protein Synthesis

Cell-free protein expression of norovirus virus-like-particles.

scholarly journals Escherichia coli-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles

Vaccines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 193
Author(s):  
Noelle Colant ◽  
Beatrice Melinek ◽  
Stefanie Frank ◽  
William Rosenberg ◽  
Daniel G. Bracewell
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Plasmid Vector ◽  
Core Antigen ◽  
Rich Region ◽  
Particle Assembly ◽  
Free Protein ◽  
Virus Like Particles ◽  
Vector Backbone ◽  
Cell Free Protein Synthesis

Tandem-core hepatitis B core antigen (HBcAg) virus-like particles (VLPs), in which two HBcAg monomers are joined together by a peptide linker, can be used to display two different antigens on the VLP surface. We produced universal influenza vaccine candidates that use this scaffold in an Escherichia coli-based cell-free protein synthesis (CFPS) platform. We then used the CFPS system to rapidly test modifications to the arginine-rich region typically found in wild-type HBcAg, the peptide linkers around the influenza antigen inserts, and the plasmid vector backbone to improve titer and quality. Using a minimal plasmid vector backbone designed for CFPS improved titers by at least 1.4-fold over the original constructs. When the linker lengths for the influenza inserts were more consistent in length and a greater variety of codons for glycine and serine were utilized, titers were further increased to over 70 μg/mL (4.0-fold greater than the original construct) and the presence of lower molecular weight product-related impurities was significantly reduced, although improvements in particle assembly were not seen. Furthermore, any constructs with the C-terminal arginine-rich region removed resulted in asymmetric particles of poor quality. This demonstrates the potential for CFPS as a screening platform for VLPs.

scholarly journals Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40588-40596
Author(s):  
Tony Köhler ◽  
Thomas Heida ◽  
Sandra Hoefgen ◽  
Niclas Weigel ◽  
Vito Valiante ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Genetic Information ◽  
In Situ Immobilization ◽  
Bottom Up ◽  
Free Protein ◽  
Malonyl Coa ◽  
Cell Free Protein Synthesis

We describe a bottom-up approach towards functional enzymes utilizing microgels as carriers for genetic information that enable cell-free protein synthesis, in situ immobilization, and utilization of functional deGFP-MatB.

scholarly journals Multivariate statistical data analysis of cell‐free protein synthesis toward monitoring and control

AIChE Journal ◽  
2021 ◽  
Author(s):  
Carlos A. Duran‐Villalobos ◽  
Olotu Ogonah ◽  
Beatrice Melinek ◽  
Daniel G. Bracewell ◽  
Trevor Hallam ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Data Analysis ◽  
Statistical Data ◽  
Monitoring And Control ◽  
Statistical Data Analysis ◽  
Multivariate Statistical ◽  
Free Protein ◽  
And Control ◽  
Cell Free Protein Synthesis

scholarly journals Cell-free riboswitches

2021 ◽  
Author(s):  
Takeshi Tabuchi ◽  
Yohei Yokobayashi
Keyword(s):  
Protein Synthesis ◽  
The State ◽  
Future Directions ◽  
Free Protein ◽  
Current Perspective ◽  
Gene Switches ◽  
A Current ◽  
Cell Free Protein Synthesis

Synthetic riboswitches can be used as chemical gene switches in cell-free protein synthesis systems. We provide a current perspective on the state of cell-free riboswitch technologies and their future directions.

scholarly journals Development and comparison of cell-free protein synthesis systems derived from typical bacterial chassis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Liyuan Zhang ◽  
Xiaomei Lin ◽  
Ting Wang ◽  
Wei Guo ◽  
Yuan Lu
Keyword(s):  
Protein Synthesis ◽  
Protein Production ◽  
Influential Factors ◽  
Competent Cell ◽  
Free Protein ◽  
Application Range ◽  
E Coli ◽  
Short Time ◽  
Cell Free Protein Synthesis

AbstractCell-free protein synthesis (CFPS) systems have become an ideal choice for pathway prototyping, protein production, and biosensing, due to their high controllability, tolerance, stability, and ability to produce proteins in a short time. At present, the widely used CFPS systems are mainly based on Escherichia coli strain. Bacillus subtilis, Corynebacterium glutamate, and Vibrio natriegens are potential chassis cells for many biotechnological applications with their respective characteristics. Therefore, to expand the platform of the CFPS systems and options for protein production, four prokaryotes, E. coli, B. subtilis, C. glutamate, and V. natriegens were selected as host organisms to construct the CFPS systems and be compared. Moreover, the process parameters of the CFPS system were optimized, including the codon usage, plasmid synthesis competent cell selection, plasmid concentration, ribosomal binding site (RBS), and CFPS system reagent components. By optimizing and comparing the main influencing factors of different CFPS systems, the systems can be optimized directly for the most influential factors to further improve the protein yield of the systems. In addition, to demonstrate the applicability of the CFPS systems, it was proved that the four CFPS systems all had the potential to produce therapeutic proteins, and they could produce the receptor-binding domain (RBD) protein of SARS-CoV-2 with functional activity. They not only could expand the potential options for in vitro protein production, but also could increase the application range of the system by expanding the cell-free protein synthesis platform.

scholarly journals De novo expression and antibacterial potential of four lactoferricin peptides in cell-free protein synthesis system

2021 ◽  
Vol 29 ◽  
pp. e00583
Author(s):  
Nawal Abd El-Baky ◽  
Maie Ahmed Elkhawaga ◽  
Eman Shawky Abdelkhalek ◽  
Mona Mohammed Sharaf ◽  
Elrashdy Mustafa Redwan ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
De Novo ◽  
Synthesis System ◽  
Free Protein ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis ◽  
Antibacterial Potential
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