Impact of gene vector design on the control of recombinant monoclonal antibody production by chinese hamster ovary cells

2011 ◽  
Vol 27 (6) ◽  
pp. 1689-1699 ◽  
Author(s):  
Sarah L. Davies ◽  
Peter M. O'Callaghan ◽  
Jane McLeod ◽  
Leon P. Pybus ◽  
Yun Hee Sung ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Gene Vector ◽  
Monoclonal Antibody Production ◽  
Ovary Cells ◽  
Recombinant Monoclonal Antibody

Optimization of chemically defined feed media for monoclonal antibody production in Chinese hamster ovary cells

2015 ◽  
Vol 120 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Shohei Kishishita ◽  
Satoshi Katayama ◽  
Kunihiko Kodaira ◽  
Yoshinori Takagi ◽  
Hiroki Matsuda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Monoclonal Antibody Production ◽  
Ovary Cells

Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells

2013 ◽  
Vol 111 (2) ◽  
pp. 372-385 ◽  
Author(s):  
Leon P. Pybus ◽  
Greg Dean ◽  
Nathan R. West ◽  
Andrew Smith ◽  
Olalekan Daramola ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Monoclonal Antibody Production ◽  
Ovary Cells

scholarly journals 4-(2,5-Dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culture

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250416
Author(s):  
Yuichi Aki ◽  
Yuta Katsumata ◽  
Hirofumi Kakihara ◽  
Koichi Nonaka ◽  
Kenshu Fujiwara
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Chemical Compounds ◽  
Ovary Cell ◽  
Monoclonal Antibody Production ◽  
Ovary Cells

There is a continuous demand to improve monoclonal antibody production for medication supply and medical cost reduction. For over 20 years, recombinant Chinese hamster ovary cells have been used as a host in monoclonal antibody production due to robustness, high productivity and ability to produce proteins with ideal glycans. Chemical compounds, such as dimethyl sulfoxide, lithium chloride, and butyric acid, have been shown to improve monoclonal antibody production in mammalian cell cultures. In this study, we aimed to discover new chemical compounds that can improve cell-specific antibody production in recombinant Chinese hamster ovary cells. Out of the 23,227 chemicals screened in this study, 4-(2,5-dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide was found to increase monoclonal antibody production. The compound suppressed cell growth and increased both cell-specific glucose uptake rate and the amount of intracellular adenosine triphosphate during monoclonal antibody production. In addition, the compound also suppressed the galactosylation on a monoclonal antibody, which is a critical quality attribute of therapeutic monoclonal antibodies. Therefore, the compound might also be used to control the level of the galactosylation for the N-linked glycans. Further, the structure-activity relationship study revealed that 2,5-dimethylpyrrole was the most effective partial structure of 4-(2,5-dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide on monoclonal antibody production. Further structural optimization of 2,5-dimethylpyrrole derivatives could lead to improved production and quality control of monoclonal antibodies.

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