Protein recovery from surfactant precipitation

2011 ◽  
Vol 27 (6) ◽  
pp. 1614-1622 ◽  
Author(s):  
Shu Ian Cheng ◽  
David C. Stuckey
Keyword(s):  
Protein Recovery ◽  
Surfactant Precipitation

OFFGEL and GELFrEE fractionation: Novel liquid-phase protein recovery strategies in proteomics studies

2021 ◽  
pp. 116282
Author(s):  
Rituparna Banerjee ◽  
Naveena B. Maheswarappa ◽  
Sowmya Dasoju ◽  
Sushma S. Andhe
Keyword(s):  
Liquid Phase ◽  
Protein Recovery ◽  
Recovery Strategies ◽  
Phase Protein

scholarly journals Protein recovery as a resource from waste specifically via membrane technology—from waste to wonder

2021 ◽  
Vol 28 (8) ◽  
pp. 10262-10282
Author(s):  
Kanwal Shahid ◽  
Varsha Srivastava ◽  
Mika Sillanpää
Keyword(s):  
Short Communication ◽  
Membrane Separation ◽  
Animal Feed ◽  
Membrane Technology ◽  
Future Research ◽  
Protein Recovery ◽  
World Population ◽  
Waste Streams ◽  
Novel Technologies ◽  
Efficient Extraction

AbstractEconomic growth and the rapid increase in the world population has led to a greater need for natural resources, which in turn, has put pressure on said resources along with the environment. Water, food, and energy, among other resources, pose a huge challenge. Numerous essential resources, including organic substances and valuable nutrients, can be found in wastewater, and these could be recovered with efficient technologies. Protein recovery from waste streams can provide an alternative resource that could be utilized as animal feed. Membrane separation, adsorption, and microbe-assisted protein recovery have been proposed as technologies that could be used for the aforementioned protein recovery. This present study focuses on the applicability of different technologies for protein recovery from different wastewaters. Membrane technology has been proven to be efficient for the effective concentration of proteins from waste sources. The main emphasis of the present short communication is to explore the possible strategies that could be utilized to recover or restore proteins from different wastewater sources. The presented study emphasizes the applicability of the recovery of proteins from various waste sources using membranes and the combination of the membrane process. Future research should focus on novel technologies that can help in the efficient extraction of these high-value compounds from wastes. Lastly, this short communication will evaluate the possibility of integrating membrane technology. This study will discuss the important proteins present in different industrial waste streams, such as those of potatoes, poultry, dairy, seafood and alfalfa, and the possible state of the art technologies for the recovery of these valuable proteins from the wastewater. Graphical abstract

Protein Recovery after Concentration of Bronchoalveolar Lavage Fluid by Ultrafiltrations

1985 ◽  
Vol 68 (s11) ◽  
pp. 26P-26P
Author(s):  
S.C. Afford ◽  
D. Burnett ◽  
J. Kramps ◽  
R.A. Stockley
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Protein Recovery

A reagent to facilitate protein recovery from cells and tissues

2012 ◽  
Vol 2 (5) ◽  
pp. 297-304 ◽  
Author(s):  
Byeong Hee Hwang ◽  
Nishit Doshi ◽  
Kenneth Y. Tsai ◽  
Samir Mitragotri
Keyword(s):  
Protein Recovery

Generic application of an aqueous two-phase process for protein recovery from animal blood

2000 ◽  
Vol 35 (7) ◽  
pp. 665-673 ◽  
Author(s):  
Marco Rito-Palomares ◽  
Christopher Dale ◽  
Andrew Lyddiatt
Keyword(s):  
Protein Recovery ◽  
Two Phase ◽  
Animal Blood ◽  
Phase Process

Effects of Enzyme Types and Extraction Conditions on Protein Recovery and Antioxidant Properties of Hydrolysed Proteins Derived from Defatted Lemna minor

2021 ◽  
Author(s):  
Hai Chi Tran ◽  
Hong Anh Thi Le ◽  
Thanh Thanh Le ◽  
Van Man Phan
Keyword(s):  
Enzymatic Hydrolysis ◽  
Antioxidant Activities ◽  
Lemna Minor ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Good Method ◽  
Alkaline Treatment ◽  
Protein Recovery ◽  
Hydrolysis Time ◽  
Hydrolysis Degree

Lemna minor (L. minor), the common duckweed, contains a high protein substance and is considered as a good source of potential bioactive peptides. The objective of this study is to investigate the effect of enzymatic hydrolysis times (60–180 min) and enzyme concentrations (0.5–3.5%v/w) with Alcalase and Flavourzyme on the recovery, hydrolysis degree (DH), and antioxidant properties of peptides derived from defatted L. minor. The protein recovery, hydrolysis degree (DH), and antioxidant activities obtained by enzymatic were compared with the alkaline treatment method. The results showed that the protein recovery, DH values, and antioxidant activities were enhanced by increasing the enzyme concentration and hydrolysis time. Specifically, the recovery of protein and DH values reached the highest level after the enzymatic hydrolysis by Flavourzyme or Alcalase at 1.5 v/w enzyme for 120 min. At the same enzymatic hydrolysis condition, the samples hydrolyzed by Flavourzyme had a higher inhibitory effect on the ABTS•+ and DPPH•+ radical scavenging than those hydrolyzed by Alcalase and the alkaline treatment. Further study also showed that the DH values, amino acid contents, and antioxidant activities of the protein extracts were positively correlated. Thus, the extractions with Flavourzyme and Alcalse were a good method to produce a significant amount of amino acids and smaller peptides.

scholarly journals Optimisation of Protein Recovery from Arthrospira platensis by Ultrasound-Assisted Isoelectric Solubilisation/Precipitation

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1586
Author(s):  
Ana Sánchez-Zurano ◽  
Ainoa Morillas-España ◽  
Cynthia Victoria González-López ◽  
Tomás Lafarga
Keyword(s):  
Food Industry ◽  
Functional Foods ◽  
Osmotic Shock ◽  
Arthrospira Platensis ◽  
Extraction Time ◽  
Protein Recovery ◽  
Solvent Ratio ◽  
Total Proteins ◽  
Cell Wall Disruption ◽  
Pre Treatment

A response surface methodology was used to optimise the solubilisation and precipitation of proteins from the cyanobacterium Arthrospira platensis. Two separate experiments were designed and conducted in a sequential manner. Protein solubilisation was affected by pH, extraction time, and biomass to solvent ratio (p < 0.001). Although spray-drying and the osmotic shock suffered when resuspending the dried biomass into distilled water led to a certain degree of cell wall disruption, the amount of protein that could be solubilised without an additional disruption step was in the range 30–60%. Sequential extractions improved protein solubilisation by less than 5%. For this reason, a pre-treatment based on sonication (400 W, 24 kHz, 2 min) had to be used, allowing the solubilisation of 96.2% of total proteins. Protein precipitation was affected by both pH and extraction time (p < 0.001). The optimised precipitation conditions, which were pH 3.89 over 45 min, led to a protein recovery of 75.2%. The protein content of the extract was close to 80%, which could be further increased by using different purification steps. The proteins extracted could be used in the food industry as technofunctional ingredients or as a source of bioactive hydrolysates and peptides for functional foods and nutraceuticals.

scholarly journals Oxidative Stress Impairs the Heat Stress Response and Delays Unfolded Protein Recovery

PLoS ONE ◽  
2009 ◽  
Vol 4 (11) ◽  
pp. e7719 ◽  
Author(s):  
Masaaki Adachi ◽  
Yaohua Liu ◽  
Kyoko Fujii ◽  
Stuart K. Calderwood ◽  
Akira Nakai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Stress ◽  
Stress Response ◽  
Protein Recovery ◽  
Heat Stress Response ◽  
Unfolded Protein
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