scholarly journals Analysis of aggregate size as a process variable affecting paclitaxel accumulation in Taxus suspension cultures

2011 ◽  
Vol 27 (5) ◽  
pp. 1365-1372 ◽  
Author(s):  
Martin E. Kolewe ◽  
Michael A. Henson ◽  
Susan C. Roberts
Keyword(s):  
Aggregate Size ◽  
Process Variable ◽  
Suspension Cultures ◽  
Paclitaxel Accumulation

scholarly journals Contribution of taxane biosynthetic pathway gene expression to observed variability in paclitaxel accumulation in Taxus suspension cultures

2012 ◽  
Vol 7 (3) ◽  
pp. 418-427 ◽  
Author(s):  
Rohan A. Patil ◽  
Martin E. Kolewe ◽  
Jennifer Normanly ◽  
Elsbeth L. Walker ◽  
Susan C. Roberts
Keyword(s):  
Gene Expression ◽  
Biosynthetic Pathway ◽  
Suspension Cultures ◽  
Pathway Gene ◽  
Paclitaxel Accumulation

Improved assessment of aggregate size in Taxus plant cell suspension cultures using laser diffraction

2012 ◽  
Vol 12 (6) ◽  
pp. 595-602 ◽  
Author(s):  
Thomas Wucherpfennig ◽  
Jana Schilling ◽  
Dominik Sieblitz ◽  
Matthias Pump ◽  
Kai Schütte ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Plant Cell ◽  
Aggregate Size ◽  
Cell Suspension Cultures ◽  
Laser Diffraction ◽  
Suspension Cultures ◽  
Plant Cell Suspension Cultures ◽  
Plant Cell Suspension

scholarly journals The Interface amongst Conserved and Specialized Pathways in Non-Paclitaxel and Paclitaxel Accumulating Taxus Cultures

Metabolites ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 688
Author(s):  
Michelle McKee ◽  
Sarah Wilson ◽  
Susan Roberts
Keyword(s):  
Methyl Jasmonate ◽  
Plant Cell ◽  
Systems Approach ◽  
Aggregate Size ◽  
Specialized Metabolism ◽  
Phenylpropanoid Biosynthesis ◽  
Culture Growth ◽  
The Relationship ◽  
Metabolite Content ◽  
Paclitaxel Accumulation

Plant cell cultures derived from Taxus are used to produce valuable metabolites like paclitaxel, a chemotherapeutic drug. Methyl jasmonate elicitation enhances paclitaxel accumulation, but also inhibits culture growth and increases phenylpropanoid biosynthesis, two side effects that detract from taxane accumulation. To understand the connection between all of these processes, a systems approach is applied to investigate cell-wide metabolism in Taxus. Non-paclitaxel and paclitaxel accumulating cultures were elicited over single and multi-generational periods, and subsequent changes in conserved and specialized metabolism were quantified. Methyl jasmonate typically resulted in decreased growth and increased metabolite content in paclitaxel accumulating cultures. Conversely, elicitation typically resulted in either no change or decrease in accumulation of metabolites in the non-paclitaxel accumulating cultures. In both sets of cultures, variability was seen in the response to methyl jasmonate across generations of cell growth. Consolidation of these data determined that paclitaxel accumulation and basal levels of phenolic and flavonoid compounds are indirectly correlated with aggregate size. These approaches assess alternative metabolic pathways that are linked to paclitaxel biosynthesis and provide a comprehensive strategy to both understand the relationship between conserved and specialized metabolism in plants and in the design of strategies to increase natural product yields in plant cell culture.

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