High-density microcarrier cell cultures for influenza virus production

2011 ◽  
Vol 27 (1) ◽  
pp. 241-250 ◽  
Author(s):  
A. Bock ◽  
J. Schulze-Horsel ◽  
J. Schwarzer ◽  
E. Rapp ◽  
Y. Genzel ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Cell Cultures ◽  
Virus Production ◽  
High Density

scholarly journals Exacerbation of Influenza Virus Infections in Mice by Intranasal Treatments and Implications for Evaluation of Antiviral Drugs

2012 ◽  
Vol 56 (12) ◽  
pp. 6328-6333 ◽  
Author(s):  
Donald F. Smee ◽  
Mark von Itzstein ◽  
Beenu Bhatt ◽  
E. Bart Tarbet
Keyword(s):  
Influenza Virus ◽  
Influenza A ◽  
H1n1 Virus ◽  
Antiviral Agent ◽  
Virus Production ◽  
Lung Pathology ◽  
Virus Infections ◽  
Challenge Dose ◽  
Virus Challenge ◽  
Time To Death

ABSTRACTCompounds lacking oral activity may be delivered intranasally to treat influenza virus infections in mice. However, intranasal treatments greatly enhance the virulence of such virus infections. This can be partially compensated for by giving reduced virus challenge doses. These can be 100- to 1,000-fold lower than infections without such treatment and still cause equivalent mortality. We found that intranasal liquid treatments facilitate virus production (probably through enhanced virus spread) and that lung pneumonia was delayed by only 2 days relative to a 1,000-fold higher virus challenge dose not accompanied by intranasal treatments. In one study, zanamivir was 90 to 100% effective at 10 mg/kg/day by oral, intraperitoneal, and intramuscular routes against influenza A/California/04/2009 (H1N1) virus in mice. However, the same compound administered intranasally at 20 mg/kg/day for 5 days gave no protection from death although the time to death was significantly delayed. A related compound, Neu5Ac2en (N-acetyl-2,3-dehydro-2-deoxyneuraminic acid), was ineffective at 100 mg/kg/day. Intranasal zanamivir and Neu5Ac2en were 70 to 100% protective against influenza A/NWS/33 (H1N1) virus infections at 0.1 to 10 and 30 to 100 mg/kg/day, respectively. Somewhat more difficult to treat was A/Victoria/3/75 virus that required 10 mg/kg/day of zanamivir to achieve full protection. These results illustrate that treatment of influenza virus infections by the intranasal route requires consideration of both virus challenge dose and virus strain in order to avoid compromising the effectiveness of a potentially useful antiviral agent. In addition, the intranasal treatments were shown to facilitate virus replication and promote lung pathology.

scholarly journals The mitogenic effect of platelet-derived growth factor in human hepatic stellate cells requires calcium influx

1995 ◽  
Vol 269 (5) ◽  
pp. C1133-C1139 ◽  
Author(s):  
P. Failli ◽  
C. Ruocco ◽  
R. De Franco ◽  
A. Caligiuri ◽  
A. Gentilini ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Cultures ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
High Density ◽  
Platelet Derived Growth Factor ◽  
Low Density ◽  
Plateau Phase ◽  
Time Dynamics ◽  
High Density Cell

Platelet-derived growth factor (PDGF) is a key mitogen for hepatic stellate cells (HSC) and has been shown to be implicated in liver tissue repair and fibrogenesis. In this study the relationship between PDGF-induced intracellular Ca2+ concentration ([Ca2+]i) increase and mitogenesis in cultured human HSC was evaluated. In high-density cell cultures (80-90% subconfluence), PDGF induced a significant increase in [Ca2+]i, characterized by a short-lasting peak phase, which was followed by a long-lasting plateau phase. The plateau phase was abolished in the absence of extracellular Ca2+. However, in low-density cell cultures (30-40% subconfluence), the plateau phase was absent or markedly less pronounced. In parallel sets of experiments, PDGF was significantly less effective in inducing mitogenesis in low-density cell cultures than in high-density cell cultures and was totally ineffective in the absence of extracellular Ca2+. These results suggest that 1) spatial and time dynamics of PDGF-induced [Ca2+]i increase are dependent on cell density and 2) PDGF-induced mitogenesis requires extracellular Ca2+ influx.

Design and application of NMR-compatible bioreactor circuits for extended perfusion of high-density mammalian cell cultures

1993 ◽  
Vol 6 (1) ◽  
pp. 95-104 ◽  
Author(s):  
R. J. Gillies ◽  
J.-P. Galons ◽  
K. A. McGovern ◽  
P. G. Scherer ◽  
Y.-H. Lien ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
High Density ◽  
Mammalian Cell Cultures ◽  
Design And Application

scholarly journals PKCdelta is a positive regulator of chondrogenesis in chicken high density micromass cell cultures

Biochimie ◽  
2011 ◽  
Vol 93 (2) ◽  
pp. 149-159 ◽  
Author(s):  
Csaba Matta ◽  
Tamás Juhász ◽  
Zsolt Szíjgyártó ◽  
Bernadett Kolozsvári ◽  
Csilla Somogyi ◽  
...  
Keyword(s):  
Cell Cultures ◽  
High Density ◽  
Positive Regulator

Active High-Density Electrode Arrays: Technology and Applications in Neuronal Cell Cultures

2019 ◽  
pp. 253-273 ◽  
Author(s):  
Davide Lonardoni ◽  
Hayder Amin ◽  
Stefano Zordan ◽  
Fabio Boi ◽  
Aziliz Lecomte ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Neuronal Cell ◽  
High Density ◽  
Electrode Arrays ◽  
Neuronal Cell Cultures

Cellular changes accompanying the growth of influenza virus in bovine cell cultures

1962 ◽  
Vol 84 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Janet S. F. Niven ◽  
J. A. Armstrong ◽  
Brigid M. Balfour ◽  
H. G. Klemperer ◽  
D. A. J. Tyrrell
Keyword(s):  
Influenza Virus ◽  
Cell Cultures ◽  
Cellular Changes ◽  
Bovine Cell

CAP, a new human suspension cell line for influenza virus production

2012 ◽  
Vol 97 (1) ◽  
pp. 111-122 ◽  
Author(s):  
Yvonne Genzel ◽  
Ilona Behrendt ◽  
Jana Rödig ◽  
Erdmann Rapp ◽  
Claudia Kueppers ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Cell Line ◽  
Virus Production ◽  
Suspension Cell ◽  
Suspension Cell Line
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