Fed-batch bioreactor performance and cell line stability evaluation of the artificial chromosome expression technology expressing an IgG1 in chinese hamster ovary cells

2010 ◽  
Vol 27 (1) ◽  
pp. 201-208 ◽  
Author(s):  
Rodney G. Combs ◽  
Erwin Yu ◽  
Susanna Roe ◽  
Michele Bailey Piatchek ◽  
Heather L. Jones ◽  
...  
Keyword(s):  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Artificial Chromosome ◽  
Stability Evaluation ◽  
Fed Batch ◽  
Ovary Cells ◽  
Batch Bioreactor ◽  
Bioreactor Performance

scholarly journals Dissecting N-Glycosylation Dynamics in Chinese Hamster Ovary Cells Fed-batch Cultures using Time Course Omics Analyses

iScience ◽  
2019 ◽  
Vol 12 ◽  
pp. 102-120 ◽  
Author(s):  
Madhuresh Sumit ◽  
Sepideh Dolatshahi ◽  
An-Hsiang Adam Chu ◽  
Kaffa Cote ◽  
John J. Scarcelli ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Time Course ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Fed Batch ◽  
Ovary Cells ◽  
Batch Cultures

Identification and control of novel growth inhibitors in fed-batch cultures of Chinese hamster ovary cells

2017 ◽  
Vol 114 (8) ◽  
pp. 1779-1790 ◽  
Author(s):  
Bhanu Chandra Mulukutla ◽  
Jaitashree Kale ◽  
Taylor Kalomeris ◽  
Michaela Jacobs ◽  
Gregory W. Hiller
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Growth Inhibitors ◽  
Fed Batch ◽  
Ovary Cells ◽  
Batch Cultures ◽  
And Control

Metabolic engineering of Chinese hamster ovary cells towards reduced biosynthesis and accumulation of novel growth inhibitors in fed-batch cultures

2019 ◽  
Vol 54 ◽  
pp. 54-68 ◽  
Author(s):  
Bhanu Chandra Mulukutla ◽  
Jeffrey Mitchell ◽  
Pauline Geoffroy ◽  
Cameron Harrington ◽  
Manisha Krishnan ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Growth Inhibitors ◽  
Fed Batch ◽  
Ovary Cells ◽  
Batch Cultures

scholarly journals Validation and identification of reference genes in Chinese hamster ovary cells for Fc-fusion protein production

2020 ◽  
Vol 245 (8) ◽  
pp. 690-702
Author(s):  
Xiaonan Ma ◽  
Ling Zhang ◽  
Luming Zhang ◽  
Chenglong Wang ◽  
Xiaorui Guo ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Chinese Hamster Ovary ◽  
Reference Genes ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Expression Stability ◽  
Fed Batch ◽  
Ovary Cells ◽  
Fc Fusion Protein

Chinese hamster ovary cells are the predominant cell lines used for bio-therapeutic production. Real-time quantitative PCR (RT-qPCR) and transcriptomics are powerful tools to understand and optimize the Chinese hamster ovary cells for higher productivity or better control of product qualities. Reliable reference genes, which were proved to be experiment-specific, are critical yardsticks. In this study, we compared expression stability of 20 candidate reference genes at mRNA level, including commonly used housekeeping genes, previous literature reported genes in Chinese hamster ovary cells producing an intact antibody, and new candidates suggested by our RNA-seq transcriptomic database, in RT-qPCR reactions in Fc-fusion protein-producing Chinese hamster ovary cells with various productivity during long-term cultivation and fed-batch cultures at 26 different conditions. geNorm, NormFinder, BestKeeper, and ΔCt programs and methods were utilized to analyze the gene expression stability and gave an overall ranking. Akr1a1, Gpx1, and Aprt in long-term cultivation and Akr1a1, Rps16 in fed-batch culture, which have not been reported previously, exhibited the highest stability of gene expression, while Pabpn1, Hirip3, and Actb in both sets of experiments together with Atp5f1 in long-term passage process showed the weakest stability. The results were then validated using GLP1-Fc (Glucagon-like peptide-1 Fc fusion protein) gene as the target with determined expression level which were doubly confirmed by both absolute RT-qPCR and confocal microscopy. These new references should be considered for the investigations on Chinese hamster ovary cells in related research. Impact statement In order to reveal potential genotype-phenotype relationship, RT-qPCR reactions are frequently applied which require validated and reliable reference genes. With the investigation on long-term passage and fed-batch cultivation of CHO cells producing an Fc-fusion protein, four new reference genes–Akr1a1, Gpx1, Aprt, and Rps16, were identified from 20 candidates with the aid of geNorm, NormFinder, BestKeeper, and ΔCt programs and methods. This article provided more verified options in reference gene selection in related research on CHO cells.

scholarly journals Enhancement of cytotoxicities of ricin and Pseudomonas toxin in Chinese hamster ovary cells by nigericin.

1981 ◽  
Vol 1 (6) ◽  
pp. 552-559 ◽  
Author(s):  
B Ray ◽  
H C Wu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Synthesis ◽  
Cell Line ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Diphtheria Toxin ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Pseudomonas Aeruginosa Exotoxin

Nigericin and monensin, ionophores for Na+ and K+, have been found to enhance the cytotoxicities of abrin, ricin, and Pseudomonas aeruginosa exotoxin A in Chinese hamster ovary (CHO) cells. They do not affect the cytotoxicity of diphtheria toxin in the same cell line. Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the presence of nigericin. Inhibition of protein synthesis in CHO cells by ricin or Pseudomonas toxin is also enhanced by preculture of CHO cells in the presence of nigericin. These results suggest a common step in the intoxication process of ricin and Pseudomonas toxin, the rate of which is facilitated by pretreatment with nigericin. This step is, however, not shared by the intoxication of CHO cells with diphtheria toxin.

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