Strategies to enhance cell growth and achieve high-level oil production of a Chlorella vulgaris isolate

2010 ◽  
Vol 26 (3) ◽  
pp. 679-686 ◽  
Author(s):  
Chun-Yen Chen ◽  
Kuei-Ling Yeh ◽  
Huei-Meei Su ◽  
Yung-Chung Lo ◽  
Wen-Ming Chen ◽  
...  
Keyword(s):  
Cell Growth ◽  
Chlorella Vulgaris ◽  
Oil Production ◽  
Enhance Cell Growth ◽  
High Level

Characterization of cellular development and fatty acid accumulation in thraustochytrid Aurantiochytrium strains of Taiwan

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Natarajan Velmurugan ◽  
Yesupatham Sathishkumar ◽  
Shashanka Sonowal ◽  
Ka-Lai Pang ◽  
Yang Soo Lee
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Flow Cytometry ◽  
Cell Growth ◽  
Intracellular Lipid ◽  
Fatty Acid Accumulation ◽  
Transmission Electron ◽  
Intracellular Lipid Accumulation ◽  
High Level

Abstract Long-chain saturated and polyunsaturated fatty acids of two new thraustochytrid isolates cultured from Taiwan mangroves, Aurantiochytrium sp. IMB169 and Aurantiochytrium sp. IMB171, were characterized through their cell growth and development in relation to their intracellular lipid accumulation using transmission electron microscopy. Flow cytometry in combination with the lipophilic fluorescent dye BODIPY 505/515 was used to stain and characterize intracellular lipid bodies in the two isolates. The transmission electron microscopy and flow cytometry analyses revealed a progressive accumulation of lipid products in IMB169 and IMB171. Further, selective BODIPY stained cells were successfully separated and enriched using flow cytometry at single cell level. Among the two isolates, IMB169 was found to produce a high level of docosahexaenoic acid. The qualitative and analytical results obtained using electron microscopy and flow cytometry studies were validated by gas chromatography (GC). In addition, a quantitative baseline was established using cell growth, flow cytometry and GC analyses for developing an efficient bioprocessing methodology to selectively enrich thraustochytrids phenotypes with desirable characteristics.

The effects of choline derivatives on the growth and lipid content of continuously cultured Chlorella vulgaris

1984 ◽  
Vol 62 (1) ◽  
pp. 81-85 ◽  
Author(s):  
D. M. Orcutt ◽  
G. A. Davis ◽  
L. D. Moore
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Cell Growth ◽  
Chlorella Vulgaris ◽  
Trimethylammonium Chloride ◽  
Effective Inhibitor ◽  
Lipid Fatty Acid ◽  
Subsequent Addition ◽  
Qualitative Changes

(2-Chloroethyl)trimethylammonium chloride (CCC), (2-hydroxyethyl)trimethylammonium chloride (CC), and (2-hydroxyethyl)trimethylammonium chloride phosphate (PCC) were studied relative to their effects on the growth and lipid composition of Chlorella vulgaris grown in continuous culture. Each chemical was added to a culture in the following sequence of concentrations: 10−6, 10−5, and 10−4 M. CC was the most effective inhibitor of cell growth and caused sequential growth reductions at increasing concentrations. CCC and PCC at 10−6 M initially inhibited cell growth, but growth recovery occurred with subsequent addition of higher concentrations of these chemicals. The polar lipid fatty acid (PL-FA), free fatty acid (FFA), triacylglycerol fatty acid (TAG-FA), and paraffinic hydrocarbon (PH) composition was determined for cells treated with each chemical. CCC at 10−5 and 10−4 M caused significant increases in TAG-FA. CC at 10−4 M stimulated TAG-FA, FFA, and PH production but inhibited PL-FA levels. PCC at 10−6 M significantly inhibited PL-FA concentrations but was stimulatory at 10−4 M. The reverse was true for the FFA concentrations relative to PCC treatments. Qualitative changes were also observed in the unsaturated fatty acid composition of the PL-FA and FFA lipid classes.

Issues of Social and Economic Development of Siberian Village of the Early XX Century in Works of V.G. Tyukavkin

10.12737/6230 ◽  
2014 ◽  
Vol 3 (3) ◽  
pp. 46-49
Author(s):  
Иванцова ◽  
N. Ivantsova
Keyword(s):  
Land Use ◽  
Economic Development ◽  
Land Tenure ◽  
Research Methodology ◽  
High Performance ◽  
Oil Production ◽  
Vast Array ◽  
Social And Economic Development ◽  
High Level ◽  
Soviet Historiography

The article analyzes the early works of famous Professor V.G. Tyukavkin. In particular, it reveals issues of research methodology, presents the conceptual vision of the problems of social and economic development of Siberian village of the early twentieth century. V.G. Tyukavkin was a supporter of the theory of a sufficiently high level of development of productive forces in agriculture of Siberia. Analyzing the questions of land tenure, land use, farming systems, cooperation, differentiation of the Siberian peasantry, he within the dominant methodology concludes that the region ripe for revolution. The article at the same time shows impartiality of the researcher, who, despite existing views, studied a vast array of dataand showed that Siberia had been turned into a granary of Russia demonstrating high performance and European quality (and oftensurpassed it, i.e. in oil production). The author for the first time in Soviet historiography raised the question of new forms of the organization of peasant production in Siberia – cooperation.

Effect of Chlorella vulgaris growing conditions on bio-oil production via fast pyrolysis

2014 ◽  
Vol 61 ◽  
pp. 187-195 ◽  
Author(s):  
Gianluca Belotti ◽  
Benedetta de Caprariis ◽  
Paolo De Filippis ◽  
Marco Scarsella ◽  
Nicola Verdone
Keyword(s):  
Chlorella Vulgaris ◽  
Fast Pyrolysis ◽  
Oil Production ◽  
Bio Oil ◽  
Growing Conditions
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