Baculovirus P35 protein: An overview of its applications across multiple therapeutic and biotechnological arenas

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Sudhir Sahdev ◽  
Kulvinder Singh Saini ◽  
Seyed E. Hasnain
Keyword(s):  
Baculovirus P35

Improved Resuscitation after Cardiac Arrest in Rats Expressing the Baculovirus Caspase Inhibitor Protein p35 in Central Neurons

2003 ◽  
Vol 99 (1) ◽  
pp. 112-121 ◽  
Author(s):  
Peter Vogel ◽  
Herman v.d. Putten ◽  
Erik Popp ◽  
Jakub J. Krumnikl ◽  
Peter Teschendorf ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Cerebral Ischemia ◽  
Neuronal Death ◽  
Neurologic Deficit ◽  
Global Cerebral Ischemia ◽  
Cardiac Massage ◽  
Caspase Inhibitor ◽  
Central Neurons ◽  
Cornu Ammonis ◽  
Baculovirus P35

Background Global cerebral ischemia is associated with delayed neuronal death. Given the role of caspases in apoptosis, caspase inhibitors may provide neuronal protection after cardiac arrest. To this end, the authors generated a transgenic rat line expressing baculovirus p35, a broad-spectrum caspase inhibitor, in central neurons. Its effects were evaluated on neuronal cell death and outcome after global cerebral ischemia. Methods Global cerebral ischemia was induced by cardiocirculatory arrest. After 6 min, animals were resuscitated by controlled ventilation, extrathoracic cardiac massage, epinephrine, and electrical countershocks. Neuronal death was assessed after 7 days by histologic evaluation of the hippocampal cornu ammonis 1 sector. Postischemic outcome was assessed by determination of overall survival and according to neurologic deficit scores 24 h, 3 days, and 7 days after resuscitation. Results The rate of 7-day survival after cardiac arrest for the transgenic rats (85%) was significantly higher than that for the nontransgenic controls (52%; P < 0.05). However, no differences were observed either in the number of terminal deoxynucleotidyltransferase-mediated d-uracil triphosphate-biotin nick end-labeling-positive cells or viable neurons in the cornu ammonis 1 sector or in the neurologic deficit score when comparing surviving transgenic and nontransgenic rats. These findings suggest that neuronal apoptosis after cardiac arrest is not primarily initiated by activation of caspases. Conclusion Expression of baculovirus p35 can improve survival after cardiac arrest in rats, but the mode and site of action remain to be elucidated.

Baculovirus p35 increases pancreatic β-cell resistance to apoptosis

2005 ◽  
Vol 332 (2) ◽  
pp. 550-556 ◽  
Author(s):  
Kenneth Hollander ◽  
Michal Bar-Chen ◽  
Shimon Efrat
Keyword(s):  
Pancreatic Β Cell ◽  
Cell Resistance ◽  
Β Cell ◽  
Baculovirus P35

scholarly journals Evidence for Agrobacterium-Induced Apoptosis in Maize Cells

2000 ◽  
Vol 13 (6) ◽  
pp. 649-657 ◽  
Author(s):  
Geneviève Hansen
Keyword(s):  
Cell Death ◽  
Dna Cleavage ◽  
Morphological Changes ◽  
Plant Cells ◽  
Plant Tissues ◽  
Apoptotic Pathway ◽  
Dicotyledonous Plant ◽  
Apoptotic Genes ◽  
Baculovirus P35 ◽  
Induced Apoptosis

Agrobacterium spp. can genetically transform most dicotyledonous plant cells whereas many monocot species are recalcitrant to Agrobacterium-mediated transformation. One major obstacle is that co-cultivation of Agrobacterium spp. with plant tissues often results in cell death. Report here is that, in maize tissues, this process resembles apoptosis, with characteristic DNA cleavage into oligonucleosomal fragments and morphological changes. Two anti-apoptotic genes from baculovirus, p35 and iap, had the ability to prevent the onset of apoptosis triggered by Agrobacterium spp. in maize tissues. p35 is reported to act as a direct inhibitor of a certain class of proteases (caspase) whereas iap may act upstream to prevent their activation. This evidence raises the possibility that caspase-like proteases may also be involved in the apoptotic pathway in plant cells.

scholarly journals Baculovirus P35 interacts with a subunit of human RNA polymerase II and can enhance promoter activity in human cells

2003 ◽  
Vol 84 (11) ◽  
pp. 3011-3019 ◽  
Author(s):  
David Takramah ◽  
Barbara M. Seiffert ◽  
Sophie Schaller ◽  
Marc Vigneron ◽  
Georg Häcker
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Promoter Activity ◽  
Human Cells ◽  
A Subunit ◽  
Baculovirus P35 ◽  
Enhance Promoter Activity
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