Metabolic engineering of Yarrowia lipolytica for the production of isoprene

2021 ◽  
Author(s):  
Kurshedaktar M. Shaikh ◽  
Annamma A. Odaneth
Keyword(s):  
Metabolic Engineering ◽  
Yarrowia Lipolytica

Metabolic engineering for ricinoleic acid production in the oleaginous yeast Yarrowia lipolytica

2013 ◽  
Vol 98 (1) ◽  
pp. 251-262 ◽  
Author(s):  
A. Beopoulos ◽  
J. Verbeke ◽  
F. Bordes ◽  
M. Guicherd ◽  
M. Bressy ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Ricinoleic Acid ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Oleaginous Yeast ◽  
Yeast Yarrowia Lipolytica

Metabolic engineering of β-carotene biosynthesis in Yarrowia lipolytica

2020 ◽  
Vol 42 (6) ◽  
pp. 945-956 ◽  
Author(s):  
Xin-Kai Zhang ◽  
Dan-Ni Wang ◽  
Jun Chen ◽  
Zhi-Jie Liu ◽  
Liu-Jing Wei ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Yarrowia Lipolytica ◽  
Β Carotene

scholarly journals Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Vijaydev Ganesan ◽  
Michael Spagnuolo ◽  
Ayushi Agrawal ◽  
Spencer Smith ◽  
Difeng Gao ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Genome Editing ◽  
Yarrowia Lipolytica ◽  
Gene Editing ◽  
Molecular Genetic ◽  
Industrial Applications ◽  
Cell Factory ◽  
Fuel Feed ◽  
Renewable Chemicals ◽  
Pharmaceutical Applications

AbstractYarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR–Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y. lipolytica. In this review we summarize the work to develop these tools and their demonstrated uses in engineering Y. lipolytica, discuss important subtleties and challenges to using these tools, and give our perspective on important gaps in gene/genome editing tools in Y. lipolytica.

scholarly journals Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system

2018 ◽  
Vol 102 (22) ◽  
pp. 9541-9548 ◽  
Author(s):  
Tian-Qiong Shi ◽  
He Huang ◽  
Eduard J. Kerkhoven ◽  
Xiao-Jun Ji
Keyword(s):  
Metabolic Engineering ◽  
Yarrowia Lipolytica

scholarly journals Metabolic engineering in the host Yarrowia lipolytica

2018 ◽  
Vol 50 ◽  
pp. 192-208 ◽  
Author(s):  
Ahmad M. Abdel-Mawgoud ◽  
Kelly A. Markham ◽  
Claire M. Palmer ◽  
Nian Liu ◽  
Gregory Stephanopoulos ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Yarrowia Lipolytica

An evolutionary metabolic engineering approach for enhancing lipogenesis in Yarrowia lipolytica

2015 ◽  
Vol 29 ◽  
pp. 36-45 ◽  
Author(s):  
Leqian Liu ◽  
Anny Pan ◽  
Caitlin Spofford ◽  
Nijia Zhou ◽  
Hal S. Alper
Keyword(s):  
Metabolic Engineering ◽  
Yarrowia Lipolytica ◽  
Engineering Approach

scholarly journals Application of Random Mutagenesis and Synthetic FadR Promoter for de novo Production of ω-Hydroxy Fatty Acid in Yarrowia lipolytica

2021 ◽  
Vol 9 ◽  
Author(s):  
Beom Gi Park ◽  
Junyeob Kim ◽  
Eun-Jung Kim ◽  
Yechan Kim ◽  
Joonwon Kim ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Metabolic Engineering ◽  
Palmitic Acid ◽  
Yarrowia Lipolytica ◽  
Cell Sorting ◽  
De Novo ◽  
Random Mutagenesis ◽  
Oleaginous Yeasts ◽  
Synthetic Promoters ◽  
Base Strain

As a means to develop oleaginous biorefinery, Yarrowia lipolytica was utilized to produce ω-hydroxy palmitic acid from glucose using evolutionary metabolic engineering and synthetic FadR promoters for cytochrome P450 (CYP) expression. First, a base strain was constructed to produce free fatty acids (FFAs) from glucose using metabolic engineering strategies. Subsequently, through ethyl methanesulfonate (EMS)-induced random mutagenesis and fluorescence-activated cell sorting (FACS) screening, improved FFA overproducers were screened. Additionally, synthetic promoters containing bacterial FadR binding sequences for CYP expression were designed to respond to the surge of the concentration of FFAs to activate the ω-hydroxylating pathway, resulting in increased transcriptional activity by 14 times from the third day of culture compared to the first day. Then, endogenous alk5 was screened and expressed using the synthetic FadR promoter in the developed strain for the production of ω-hydroxy palmitic acid. By implementing the synthetic FadR promoter, cell growth and production phases could be efficiently decoupled. Finally, in batch fermentation, we demonstrated de novo production of 160 mg/L of ω-hydroxy palmitic acid using FmeN3-TR1-alk5 in nitrogen-limited media. This study presents an excellent example of the production of ω-hydroxy fatty acids using synthetic promoters with bacterial transcriptional regulator (i.e., FadR) binding sequences in oleaginous yeasts.

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