Prefiltration enhances performance of sterile filtration for glycoconjugate vaccines

2021 ◽  
Author(s):  
Zhuoshi Du ◽  
Seyed Pouria Motevalian ◽  
Brenda Carrillo Conde ◽  
Kelvin Reilly ◽  
Andrew L. Zydney
Keyword(s):  
Sterile Filtration ◽  
Glycoconjugate Vaccines

Modified intermediate pore blockage model describes fouling behavior during sterile filtration of glycoconjugate vaccines

2020 ◽  
Vol 613 ◽  
pp. 118495 ◽  
Author(s):  
Parinaz Emami ◽  
Fatemeh Fallahianbijan ◽  
Erica Dinse ◽  
Seyed Pouria Motevalian ◽  
Brenda Carrillo Conde ◽  
...  
Keyword(s):  
Sterile Filtration ◽  
Pore Blockage ◽  
Glycoconjugate Vaccines

scholarly journals Effect of O-Antigen Chain Length Regulation on the Immunogenicity of Shigella and Salmonella Generalized Modules for Membrane Antigens (GMMA)

2021 ◽  
Vol 22 (3) ◽  
pp. 1309
Author(s):  
Gianmarco Gasperini ◽  
Maria Michelina Raso ◽  
Vanessa Arato ◽  
Maria Grazia Aruta ◽  
Paola Cescutti ◽  
...  
Keyword(s):  
Immune Response ◽  
Structural Characteristics ◽  
Antigen Delivery ◽  
Critical Quality Attributes ◽  
O Antigen ◽  
Alternative Approach ◽  
Length Regulation ◽  
Membrane Antigens ◽  
Glycoconjugate Vaccines ◽  
Work Supports

Recently, generalized modules for membrane antigens (GMMA) technology has been proposed as an alternative approach to traditional glycoconjugate vaccines for O-antigen delivery. Saccharide length is a well-known parameter that can impact the immune response induced by glycoconjugates both in terms of magnitude and quality. However, the criticality of O-antigen length on the immune response induced by GMMA-based vaccines has not been fully elucidated. Here, Shigella and Salmonella GMMA-producing strains were further mutated in order to display homogeneous polysaccharide populations of different sizes on a GMMA surface. Resulting GMMA were compared in mice immunization studies. Athymic nude mice were also used to investigate the involvement of T-cells in the immune response elicited. In contrast with what has been reported for traditional glycoconjugate vaccines and independent of the pathogen and the sugar structural characteristics, O-antigen length did not result in being a critical parameter for GMMA immunogenicity. This work supports the identification of critical quality attributes to optimize GMMA vaccine design and improve vaccine efficacy and gives insights on the nature of the immune response induced by GMMA.

scholarly journals Recent and Future Advances in the Chemoenzymatic Synthesis of Homogeneous Glycans for Bacterial Glycoconjugate Vaccine Development

Vaccines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1021
Author(s):  
Ayobami Adegbite ◽  
Pumtiwitt C. McCarthy
Keyword(s):  
Immune Response ◽  
Vaccine Development ◽  
Disease Outbreaks ◽  
Chemoenzymatic Synthesis ◽  
Conjugate Vaccines ◽  
Oligosaccharide Chain ◽  
Glycoconjugate Vaccines ◽  
Glycoconjugate Vaccine ◽  
The Relationship ◽  
Number Of Bacteria

Vaccines are important in preventing disease outbreaks and controlling the spread of disease in a population. A variety of vaccines exist, including subunit, recombinant, and conjugate vaccines. Glycoconjugate vaccines have been an important tool to fight against diseases caused by a number of bacteria. Glycoconjugate vaccines are often heterogeneous. Vaccines of the future are becoming more rationally designed to have a defined oligosaccharide chain length and position of conjugation. Homogenous vaccines could play an important role in assessing the relationship between vaccine structure and immune response. This review focuses on recent advances in the chemoenzymatic production of defined bacterial oligosaccharides for vaccine development with a focus on Neisseria meningitidis and selected WHO-prioritized antibacterial resistant-pathogens. We also provide some perspective on future advances in the chemoenzymatic synthesis of well-defined oligosaccharides.

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