Modulation of transmembrane pressure in manufacturing scale tangential flow filtration N‐1 perfusion seed culture

The development of a culture-free method for Salmonella screening of spent irrigation water derived from sprouting mung bean beds is described. The system used tangential flow filtration (TFF) to nonspecifically concentrate cells from large (2- to 10-liter) sample volumes. The retentate (100 ml) from the TFF was then flowed over an anti-Salmonella antibody–modified cellulose acetate membrane. The captured Salmonella was detected by reacting with a secondary anti-Salmonella and goat anti-rabbit biotin labeled antibody, followed by avidin-tagged glucose oxidase. The hydrogen peroxide generated from the enzymic oxidation of glucose was amperometrically detected at an underlying platinum electrode. It was found that 10 liters of Salmonella suspensions of 2 log CFU/ml could be concentrated to 4 log CFU/ml with 60% recovery regardless of the flow rate (112 to 511 ml/min) or transmembrane pressure (0 to 20 lb/in2) applied. The solids content of spent irrigation water negatively affected the filtration rate of TFF. This was most evident in spent irrigation water collected in the initial 24 h of the sprouting period, where the solids content was high (4,170 mg/liter) compared with samples collected at 96 h (560 mg/liter). Trials were performed using mung bean beds inoculated with different Salmonella levels (1.3 to 3.3 log CFU/g). By using the optimized TFF and flowthrough immunoassay it was possible to detect Salmonella in spent irrigation water at levels of 2.43 log CFU/ml within 4 h. The integrated concentration and detection system will provide a useful tool for sprout producers to perform in-house pathogen screening of spent irrigation water.

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Model-Based Design and Process Optimization of Continuous Single Pass Tangential Flow Filtration Focusing on Continuous Bioprocessing

Processes ◽

10.3390/pr7060317 ◽

2019 ◽

Vol 7 (6) ◽

pp. 317 ◽

Cited By ~ 6

Author(s):

Maximilian J. Huter ◽

Jochen Strube

Keyword(s):

Transmembrane Pressure ◽

Tangential Flow Filtration ◽

Single Pass ◽

Tangential Flow ◽

Model Based ◽

Physico Chemical ◽

Versatile Tool ◽

Flow Filtration ◽

Conceptual Process Design ◽

Parameter Dependent

In this study the Single-Pass-Tangential-Flow-Filtration (SPTFF) concept for continuous ultrafiltration in bioprocessing is investigated. Based on a previously validated physico-chemical model for a single ultrafiltration cassette, the transfer to a multistage SPTFF is predicted and validated experimentally by concentration steps for bovine serum albumin (BSA) and the monoclonal antibody immunoglobulin G (IgG) are compared. The model applied for the ultrafiltration membrane contains the Stagnant Film Model (SFM) for concentration polarization, as well as the Osmotic Pressure Model (OPM) and the Boundary Layer Model (BLM) for the mass transfer through the membrane. In addition, pressure drop correlations as a function of the Reynolds number are included to describe the development of the transmembrane pressure over the length of the module. The outcome of this study shows the potential to improve this multi-parameter dependent unit operation by a model-based optimization allowing significant reduction of experimental efforts and applying the Quality by Design (QbD) approach consistently. Consequently, a versatile tool for conceptual process design is presented and further application is discussed.

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Concentration of live pico- and nanoplankton by means of tangential flow filtration

Journal of Plankton Research ◽

10.1093/plankt/11.6.1213 ◽

1989 ◽

Vol 11 (6) ◽

pp. 1213-1221 ◽

Cited By ~ 10

Author(s):

Klaus-Günther Barthel ◽

Gerald Schneider ◽

Rolf Gradinger ◽

Jürgen Lenz

Keyword(s):

Tangential Flow Filtration ◽

Tangential Flow ◽

Flow Filtration

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