scholarly journals Holistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in Pichia pastoris by means of an acid pH pulse during fed‐batch fermentation

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
M. Lourdes Velez‐Suberbie ◽  
Stephen A. Morris ◽  
Kawaljit Kaur ◽  
John M. Hickey ◽  
Sangeeta B. Joshi ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Fermentation ◽  
Process Development ◽  
Vaccine Candidate ◽  
Rotavirus Vaccine ◽  
Fed Batch ◽  
Acid Ph ◽  
Holistic Process ◽  
Fed Batch Fermentation ◽  
A Subunit

Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris

2017 ◽  
Vol 43 (1) ◽  
pp. 133-141 ◽  
Author(s):  
Mahzan Md Tab ◽  
Noor Haza Fazlin Hashim ◽  
Nazalan Najimudin ◽  
Nor Muhammad Mahadi ◽  
Farah Diba Abu Bakar ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Fermentation ◽  
Large Scale ◽  
Antifreeze Protein ◽  
Scale Production ◽  
Fed Batch ◽  
Large Scale Production ◽  
Fed Batch Fermentation

Maximization of Production of Secreted Recombinant Proteins in Pichia pastoris Fed-Batch Fermentation

2008 ◽  
Vol 21 (2) ◽  
pp. 386-393 ◽  
Author(s):  
Wenhui Zhang ◽  
Jayanta Sinha ◽  
Leonard A. Smith ◽  
Mehmet Inan ◽  
Michael M. Meagher
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Proteins ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation

scholarly journals Implementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by Methanol Rate

2021 ◽  
Vol 11 (5) ◽  
pp. 12633-12641
Keyword(s):  
Pichia Pastoris ◽  
Cell Density ◽  
Batch Fermentation ◽  
Fermentation Process ◽  
Production Efficiency ◽  
Continuous Fermentation ◽  
Scale Up ◽  
Fed Batch ◽  
Simple Method ◽  
Fed Batch Fermentation

High cell density fed-batch fermentation is the main strategy for recombinant hepatitis B surface antigen (rHBsAg) production. In this study, we employed short-term continuous fermentation to optimize the cell density of recombinant Pichia pastoris (P. pastoris). After reaching the maximum specified broth volume of 5 L in the fed-batch fermentation process, the operation mode was altered into the continuous mode with a dilution rate of 0.009 1/h. We used various values of methanol inflow to examine its impact as a limiting nutrient on cell density. After reaching the steady-state point, the continuous fermentation was stopped. The process's performance was evaluated based on titer, yield, productivity, and ease of process control. According to the results, the optimal methanol inflow in the pilot-scale fermentation process was 39.9 ml/h as the cell density increased from 363 g/l wet cell weight (WCW) in the fed-batch stage to 450 g/l WCW. We could successfully scale up the fermentation process with the biomass concentration of 450 g/l without having any major issues such as excessive heat dissipation or insufficient oxygen supply. This approach is a simple method for enhancing rHBsAg production efficiency in P. pastoris without requiring any new and complex facility.

Sign in / Sign up

Export Citation Format

Share Document