Microfabricated arrays of cylindrical wells facilitate single-molecule enzymology of α-chymotrypsin

2009 ◽  
Vol 25 (4) ◽  
pp. 929-937 ◽  
Author(s):  
Angela Y. Chen ◽  
Ashish S. Jani ◽  
Lifeng Zheng ◽  
Peter J. Burke ◽  
James P. Brody
Keyword(s):  
Single Molecule ◽  
Microfabricated Arrays ◽  
Single Molecule Enzymology

Microfabricated arrays of femtoliter chambers allow single molecule enzymology

2005 ◽  
Vol 23 (3) ◽  
pp. 361-365 ◽  
Author(s):  
Yannick Rondelez ◽  
Guillaume Tresset ◽  
Kazuhito V Tabata ◽  
Hideyuki Arata ◽  
Hiroyuki Fujita ◽  
...  
Keyword(s):  
Single Molecule ◽  
Microfabricated Arrays ◽  
Single Molecule Enzymology

scholarly journals Single-Molecule Enzymology: Studies on Glutathione-S-Transferases

2011 ◽  
Vol 100 (3) ◽  
pp. 193a
Author(s):  
Abhinav Nath ◽  
William M. Atkins ◽  
Andrew D. Miranker ◽  
Elizabeth Rhoades
Keyword(s):  
Single Molecule ◽  
Glutathione S Transferases ◽  
Single Molecule Enzymology

scholarly journals Single-molecule enzymology à la Michaelis-Menten

FEBS Journal ◽  
2014 ◽  
Vol 281 (2) ◽  
pp. 518-530 ◽  
Author(s):  
Ramon Grima ◽  
Nils G. Walter ◽  
Santiago Schnell
Keyword(s):  
Single Molecule ◽  
Single Molecule Enzymology

[12] Molecular motors and single-molecule enzymology

2003 ◽  
pp. 228-245 ◽  
Author(s):  
Yoshiharu Ishii ◽  
Kazuo Kitamura ◽  
Hiroto Tanaka ◽  
Toshio Yanagida
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
Single Molecule Enzymology

scholarly journals Single-molecule enzymology: What can it tell us?

2003 ◽  
Vol 25 (4) ◽  
pp. 24-27 ◽  
Author(s):  
Clive R. Bagshaw
Keyword(s):  
Enzyme Activity ◽  
Single Molecule ◽  
Enzyme Molecule ◽  
Basic Principles ◽  
Single Enzyme ◽  
Single Molecule Enzymology

In the last decade, several methods have been developed to measure enzyme activity at the level of a single enzyme molecule. This represents a considerable technical feat, but what does it allow us to learn about enzymes? Here, the basic principles are reviewed to show that new forms of heterogeneity in activity may be revealed and evidence gained for rare states that would otherwise be swamped in bulk assays.

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