scholarly journals Identification of upstream culture conditions and harvest time parameters that affect host cell protein clearance

2019 ◽  
pp. e2805 ◽  
Author(s):  
Louisa J. Wilson ◽  
Will Lewis ◽  
Richard Kucia‐Tran ◽  
Daniel G. Bracewell
Keyword(s):  
Host Cell ◽  
Culture Conditions ◽  
Cell Protein ◽  
Harvest Time ◽  
Host Cell Protein ◽  
Protein Clearance ◽  
Time Parameters

Demonstration of Robust Host Cell Protein Clearance in Biopharmaceutical Downstream Processes

2008 ◽  
Vol 24 (3) ◽  
pp. 615-622 ◽  
Author(s):  
A.A. Shukla ◽  
C. Jiang ◽  
J. Ma ◽  
M. Rubacha ◽  
L. Flansburg ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Protein Clearance ◽  
Downstream Processes

scholarly journals A modular and adaptive mass spectrometry-based platform for support of bioprocess development toward optimal host cell protein clearance

mAbs ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 654-663 ◽  
Author(s):  
Donald E. Walker ◽  
Feng Yang ◽  
Joseph Carver ◽  
Koman Joe ◽  
David A. Michels ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Host Cell ◽  
Cell Protein ◽  
Bioprocess Development ◽  
Host Cell Protein ◽  
Protein Clearance

scholarly journals The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nicholas M. Negretti ◽  
Christopher R. Gourley ◽  
Prabhat K. Talukdar ◽  
Geremy Clair ◽  
Courtney M. Klappenbach ◽  
...  
Keyword(s):  
Host Cell ◽  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Small Gtpase ◽  
Host Cells ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Cell Binding ◽  
Actin Reorganization ◽  
Cell Cytosol

AbstractCampylobacter jejuni is a foodborne pathogen that binds to and invades the epithelial cells lining the human intestinal tract. Maximal invasion of host cells by C. jejuni requires cell binding as well as delivery of the Cia proteins (Campylobacter invasion antigens) to the host cell cytosol via the flagellum. Here, we show that CiaD binds to the host cell protein IQGAP1 (a Ras GTPase-activating-like protein), thus displacing RacGAP1 from the IQGAP1 complex. This, in turn, leads to the unconstrained activity of the small GTPase Rac1, which is known to have roles in actin reorganization and internalization of C. jejuni. Our results represent the identification of a host cell protein targeted by a flagellar secreted effector protein and demonstrate that C. jejuni-stimulated Rac signaling is dependent on IQGAP1.

Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics

2016 ◽  
Vol 18 (6) ◽  
pp. 1439-1452 ◽  
Author(s):  
Vibha Jawa ◽  
Marisa K. Joubert ◽  
Qingchun Zhang ◽  
Meghana Deshpande ◽  
Suminda Hapuarachchi ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Protein ◽  
Host Cell Protein

scholarly journals Host cell protein platform assay development for therapeutic mAb bioprocessing using mammalian cells

2015 ◽  
Vol 9 (S9) ◽  
Author(s):  
Nadine Kochanowski ◽  
Gaetan Siriez ◽  
Larissa Mukankurayija ◽  
Aurélie Delangle ◽  
Alex Murray-Smith ◽  
...  
Keyword(s):  
Host Cell ◽  
Mammalian Cells ◽  
Assay Development ◽  
Cell Protein ◽  
Host Cell Protein
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