Prokaryotic expression, in vitro folding, and molecular pharmacological characterization of the neuropeptide Y receptor type 2

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Peter Schmidt ◽  
Diana Lindner ◽  
Cindy Montag ◽  
Sandra Berndt ◽  
Annette G. Beck-Sickinger ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Prokaryotic Expression ◽  
Receptor Type ◽  
Pharmacological Characterization ◽  
Neuropeptide Y Receptor

Oxidative in vitro folding of a cysteine deficient variant of the G protein-coupled neuropeptide Y receptor type 2 improves stability at high concentration

2013 ◽  
Vol 394 (8) ◽  
pp. 1045-1056 ◽  
Author(s):  
Kristina Witte ◽  
Anette Kaiser ◽  
Peter Schmidt ◽  
Victoria Splith ◽  
Lars Thomas ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
G Protein ◽  
Significant Loss ◽  
Receptor Type ◽  
High Concentration ◽  
Neuropeptide Y Receptor ◽  
Culture Models ◽  
G Protein Coupled

Abstract In vitro folding of G protein-coupled receptors into a detergent environment represents a promising strategy for obtaining sufficient amounts of functional receptor molecules for structural studies. Typically, these preparations exhibit a poor long-term stability especially at the required high protein concentration. Here, we report a protocol for the stabilization of the Escherichia coli-expressed and subsequently folded neuropeptide Y receptor type 2. We identified the free cysteines in the receptor as one major reason for intermolecular protein aggregation. Therefore, six out of the eight cysteine residues were mutated to alanine or serine without any significant loss of functionality of the receptor as demonstrated in cell culture models. Furthermore, the disulfide bond between the remaining two cysteines was irreversibly formed by applying oxidative in vitro folding. Applying this strategy, the stability of the functionally folded Y2 receptor could be increased to 20 days at a concentration of 15 μm in a micelle environment consisting of 1,2-diheptanoyl-sn-glycero-3-phosphocholine and n-dodecyl-ß-D-maltoside.

Characterization of bladder function in a cannabinoid receptor type 2 knockout mouse in vivo and in vitro

2013 ◽  
Vol 33 (5) ◽  
pp. 566-570 ◽  
Author(s):  
Lysanne Campeau ◽  
Claudius Füllhase ◽  
Norifumi Sawada ◽  
Christian Gratzke ◽  
Petter Hedlund ◽  
...  
Keyword(s):  
Knockout Mouse ◽  
Cannabinoid Receptor ◽  
Receptor Type ◽  
Bladder Function ◽  
Cannabinoid Receptor Type 2

Cloning and pharmacological characterization of the neuropeptide Y receptor Y5 in the sea lamprey, Petromyzon marinus

Peptides ◽  
2013 ◽  
Vol 39 ◽  
pp. 64-70 ◽  
Author(s):  
Bo Xu ◽  
Görel Sundström ◽  
Shigehiro Kuraku ◽  
Ingrid Lundell ◽  
Dan Larhammar
Keyword(s):  
Neuropeptide Y ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Pharmacological Characterization ◽  
Neuropeptide Y Receptor

scholarly journals Investigation of Structural Changes Upon Ligand Binding of the Methylated Neuropeptide Y Receptor Type 2

2011 ◽  
Vol 100 (3) ◽  
pp. 9a
Author(s):  
Sandra Berndt ◽  
Peter Schmidt ◽  
Christian Berger ◽  
Annette Beck-Sickinger ◽  
Daniel Huster
Keyword(s):  
Ligand Binding ◽  
Neuropeptide Y ◽  
Structural Changes ◽  
Receptor Type ◽  
Neuropeptide Y Receptor

Structural dynamics of the ligand–receptor interaction of the neuropeptide Y receptor type 2

Neuropeptides ◽  
2016 ◽  
Vol 55 ◽  
pp. 9-10
Author(s):  
Anette Kaiser ◽  
Paul Müller ◽  
Julian Kahr ◽  
Tristan Zellmann ◽  
Holger A. Scheidt ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Structural Dynamics ◽  
Receptor Type ◽  
Receptor Interaction ◽  
Neuropeptide Y Receptor

Characterization of the Human Type 2 Neuropeptide Y Receptor Gene (NPY2R) and Localization to the Chromosome 4q Region Containing the Type 1 Neuropeptide Y Receptor Gene

Genomics ◽  
1996 ◽  
Vol 38 (3) ◽  
pp. 392-398 ◽  
Author(s):  
David A. Ammar ◽  
Deborah M. Eadie ◽  
Deborah J. Wong ◽  
Yen-Ying Ma ◽  
lee F. Kolakowski, Jr. ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Receptor Gene ◽  
Human Type ◽  
Neuropeptide Y Receptor
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