Heterologous expression and purification of active L-asparaginase I of Saccharomyces cerevisiae in Escherichia coli host

2016 ◽  
Vol 33 (2) ◽  
pp. 416-424 ◽  
Author(s):  
João H. P. M. Santos ◽  
Iris M. Costa ◽  
João V. D. Molino ◽  
Mariana S. M. Leite ◽  
Marcela V. Pimenta ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Heterologous Expression ◽  
Expression And Purification

scholarly journals The PLUTO plastidial nucleobase transporter also transports the thiamin precursor hydroxymethylpyrimidine

2018 ◽  
Vol 38 (2) ◽  
Author(s):  
Guillaume A.W. Beaudoin ◽  
Timothy S. Johnson ◽  
Andrew D. Hanson
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Root Growth ◽  
Heterologous Expression ◽  
Transporter Gene ◽  
Bacterial Genomes ◽  
Thiamin Biosynthesis ◽  
Increased Sensitivity ◽  
Arabidopsis Homolog

In plants, the hydroxymethylpyrimidine (HMP) and thiazole precursors of thiamin are synthesized and coupled together to form thiamin in plastids. Mutants unable to form HMP can be rescued by exogenous HMP, implying the presence of HMP transporters in the plasma membrane and plastids. Analysis of bacterial genomes revealed a transporter gene that is chromosomally clustered with thiamin biosynthesis and salvage genes. Its closest Arabidopsis homolog, the plastidic nucleobase transporter (PLUTO), is co-expressed with several thiamin biosynthetic enzymes. Heterologous expression of PLUTO in Escherichia coli or Saccharomyces cerevisiae increased sensitivity to a toxic HMP analog, and disrupting PLUTO in an HMP-requiring Arabidopsis line reduced root growth at low HMP concentrations. These data implicate PLUTO in plastidial transport and salvage of HMP.

scholarly journals Heterologous expression and purification of porcine fat mass andobesity-associated gene in Escherichia coli

2015 ◽  
Vol 39 ◽  
pp. 217-222 ◽  
Author(s):  
Bo ZHOU ◽  
Xiaoling CHEN ◽  
Zhiqing HUANG ◽  
Gang JIA ◽  
Guangmang LIU
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Fat Mass ◽  
Expression And Purification

Heterologous expression and purification of a marine alginate lyase in Escherichia coli

2019 ◽  
Vol 153 ◽  
pp. 97-104 ◽  
Author(s):  
Xiaoyue Sun ◽  
Wei Shen ◽  
Yanyun Gao ◽  
Menghao Cai ◽  
Mian Zhou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Alginate Lyase ◽  
Expression And Purification
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