Effect of amino acid supplementation on titer and glycosylation distribution in hybridoma cell cultures-Systems biology-based interpretation using genome-scale metabolic flux balance model and multivariate data analysis

2016 ◽  
Vol 32 (5) ◽  
pp. 1163-1173 ◽  
Author(s):  
Thomas M. Reimonn ◽  
Seo-Young Park ◽  
Cyrus D. Agarabi ◽  
Kurt A. Brorson ◽  
Seongkyu Yoon
Keyword(s):  
Data Analysis ◽  
Systems Biology ◽  
Amino Acid ◽  
Cell Cultures ◽  
Multivariate Data Analysis ◽  
Metabolic Flux ◽  
Balance Model ◽  
Amino Acid Supplementation ◽  
Flux Balance ◽  
Genome Scale

A genome‐scale nutrient minimization forecast algorithm for controlling essential amino acid levels in CHO cell cultures

2021 ◽  
Author(s):  
Yiqun Chen ◽  
Xiao Liu ◽  
Ji Young Anderson ◽  
Harnish Mukesh Naik ◽  
Venkata Gayatri Dhara ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cell Cultures ◽  
Essential Amino Acid ◽  
Cho Cell ◽  
A Genome ◽  
Amino Acid Levels ◽  
Genome Scale

Metabolic flux balance analysis during lactate and glucose concomitant consumption in HEK293 cell cultures

2018 ◽  
Vol 116 (2) ◽  
pp. 388-404 ◽  
Author(s):  
Iván Martínez‐Monge ◽  
Joan Albiol ◽  
Martí Lecina ◽  
Leticia Liste‐Calleja ◽  
Joan Miret ◽  
...  
Keyword(s):  
Flux Balance Analysis ◽  
Cell Cultures ◽  
Metabolic Flux ◽  
Hek293 Cell ◽  
Flux Balance ◽  
Balance Analysis

scholarly journals Investigation of Efficacy Enhancing and Toxicity Reducing Mechanism of Combination of Aconiti Lateralis Radix Praeparata and Paeoniae Radix Alba in Adjuvant-Induced Arthritis Rats by Metabolomics

2019 ◽  
Vol 2019 ◽  
pp. 1-15
Author(s):  
Yun-fei Xie ◽  
Wu-wen Feng ◽  
Mei-chen Liu ◽  
Jun Xie ◽  
Lei Yu ◽  
...  
Keyword(s):  
Data Analysis ◽  
Amino Acid ◽  
Therapeutic Dose ◽  
Multivariate Data Analysis ◽  
Metabolic Disorders ◽  
Multivariate Data ◽  
Toxic Dose ◽  
Adjuvant Induced Arthritis ◽  
Paeoniae Radix ◽  
Total Glucosides Of Peony

Combination of Aconiti Lateralis Radix Praeparata (FZ) and Paeoniae Radix Alba (BS) shows a significant effect in rheumatoid arthritis (RA). This study aimed to investigate the efficacy enhancing and toxicity reducing mechanism of combination of them in adjuvant-induced arthritis (AIA) rats by metabolomics. Rats were randomly divided into seven groups, including A (healthy control), B (model control), C1 (therapy group), C2 (efficacy enhancing group), D1 (toxicity group), and D2 (toxicity reducing group), and dexamethasone group was used as positive control. The plasma biochemical indexes showed that therapeutic dose of lipid-soluble alkaloids of FZ could significantly inhibit the concentrations of IL-1β, TNF-α, and IFN-γ in AIA rats, and combination with total glucosides of peony could further reduce the concentration of IL-1β. Then, UPLC-LTQ/Orbitrap MS with untargeted metabolomics was performed to identify the possible metabolites and pathways. Through multivariate data analysis of therapeutic dose groups (A vs. B vs. C1 vs. C2) and multivariate data analysis of toxic dose groups (A vs. B vs. D1 vs. D2), 10 and 7 biomarkers were identified based on biomarker analysis, respectively. After inducing AIA model, the plasma contents of spermidine, vanillylmandelic acid, catechol, and linoleate were increased significantly, and the contents of citric acid, L-tyrosine, L-phenylalanine, leucine, L-tryptophan, and uridine 5'-monophosphate (UMP) were decreased significantly. High dose of lipid-soluble alkaloids of FZ could increase the plasma contents of L-lysine, L-arginine, and deoxycholic acid, while the plasma contents of UMP, carnitine, N-formylanthranilic acid, and adenosine were decreased significantly. The pathway analysis indicated that therapeutic dose of lipid-soluble alkaloids of FZ could regulate energy and amino acid metabolic disorders in AIA rats. However, toxic dose could cause bile acid, fat, amino acid, and energy metabolic disorders. And combination with total glucosides of peony could enhance the therapeutic effects and attenuate the toxicity induced by lipid-soluble alkaloids of FZ.

scholarly journals Expanding a dynamic flux balance model of yeast fermentation to genome-scale

2011 ◽  
Vol 5 (1) ◽  
pp. 75 ◽  
Author(s):  
Felipe A Vargas ◽  
Francisco Pizarro ◽  
J Ricardo Pérez-Correa ◽  
Eduardo Agosin
Keyword(s):  
Balance Model ◽  
Yeast Fermentation ◽  
Flux Balance ◽  
Genome Scale
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