Production of rhamnolipids and diesel oil degradation by bacteria isolated from soil contaminated by petroleum

2015 ◽  
Vol 32 (2) ◽  
pp. 262-270 ◽  
Author(s):  
Giuseppe G. F. Leite ◽  
Juciane V. Figueirôa ◽  
Thiago C. M. Almeida ◽  
Jaqueline L. Valões ◽  
Walber F. Marques ◽  
...  
Keyword(s):  
Diesel Oil ◽  
Oil Degradation ◽  
Diesel Oil Degradation

scholarly journals Est16, a New Esterase Isolated from a Metagenomic Library of a Microbial Consortium Specializing in Diesel Oil Degradation

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0133723 ◽  
Author(s):  
Mariana Rangel Pereira ◽  
Gustavo Fernando Mercaldi ◽  
Thaís Carvalho Maester ◽  
Andrea Balan ◽  
Eliana Gertrudes de Macedo Lemos
Keyword(s):  
Microbial Consortium ◽  
Metagenomic Library ◽  
Diesel Oil ◽  
Oil Degradation ◽  
Diesel Oil Degradation

Isolation and Characterization of Petroleum Hydrocarbon Degrading Bacteria from the Bohai Sea, China

2014 ◽  
Vol 955-959 ◽  
pp. 728-731
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Petroleum Hydrocarbon ◽  
Bohai Sea ◽  
Diesel Oil ◽  
Contaminated Site ◽  
Oil Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Luxuriant Growth ◽  
Diesel Oil Degradation

Fourteen petroleum hydrocarbon degrading bacteria strains were isolated from oil-contaminated site. Isolated strains were able use diesel oil as sole carbon and energy source. Bacterial strain HD1 was selected due to the luxuriant growth on oil agar. The oil degradation rate of strain HD1 was analyzed using UV-spectrometry-based methods. The result showed that the rate of diesel oil degradation of 75% was observed after 14days of cultivation.

scholarly journals Enhancement diesel oil degradation by using biofilm forming bacteria on biochar

2018 ◽  
Vol 9 (1) ◽  
pp. 26-31
Author(s):  
Thi Nhi Cong Le ◽  
Thi Ngoc Mai Cung ◽  
Ngoc Huy Vu ◽  
Thi Lien Do ◽  
Thi To Uyen Do ◽  
...  
Keyword(s):  
Climate Change Mitigation ◽  
Crop Residues ◽  
Diesel Oil ◽  
Oil Degradation ◽  
Pseudomonas Sp ◽  
Wood Biomass ◽  
Porous Substance ◽  
Fine Grained ◽  
Change Mitigation ◽  
Diesel Oil Degradation

Biochar is defined as a carbon-rich, fine-grained, porous substance, which is produced by pyrolysis biomass with little or no oxygen. Biochar is usually produced from crop residues, wood biomass, animal litters, and solid wastes. Recently, biochar is increasingly receiving attention as an environmental-friendly approach, especially as a climate change mitigation strategy. Biochar is especilly demonstrated to remove diesel oil (DO) from soil and water. In this report, 4 biofilm forming bacteria including Klepsiellasp. VTD8, Pseudomonas sp. BQN21, Rhodococcussp. BN5 and Stenotropomonassp. QND8 were used to attach to biochar produced from husk to estimate the capacity of their DO removal. As the results, removal efficiency of biofilm formed by each strain VTD8, BQN21, BN5 and QND8 were 67, 73, 75 and 68 % with initial concentration of 39 g/l, respectively. On the other hand, mix species biofilm attached to husk carrier and without carrier degraded 98 and 78 %. Using husk without bacteria as absortion control, the amount of DO removal was 23 %. These results gave hint that using biochar produced from husk as carrier for biofilm forming bacteria to attach may increase efficiency of DO pollution treatment. Than sinh học (biochar) là một chất xốp có các gốc carbon và có nguồn gốc từ quá trình nhiệt phân sinh khối các loại chất thải, động, thực vật,… dưới điều kiện hạn chế oxy hoặc không có oxy.Hiện nay biochar đã được ứng dụng rộng rãi trong xử lý môi trường. Đặc biệt các biochar còn được chứng minh là có thể xử lý dầu diesel (diesel oil - DO) có trong đất và nước. Trong nghiên cứu này, chúng tôi sử dụng 4 chủng vi khuẩn tạo màng sinh học tốt là Klepsiella sp. VTD8, Pseudomonas sp. BQN21, Rhodococcus sp. BN5 và Stenotropomonas sp. QND8 để gắn lên chất mang là biochar làm từ trấu nhằm đánh giá hiệu quả xử lý DO của chúng. Kết quả cho thấy, sau 7 ngày, các chủng VTD8, BQN21, BN5 và QND8 có khả năng phân hủy 67, 73, 75 và 68 % DO với hàm lượng ban đầu là 39 g/l. Trong khi đó, hiệu suất của màng sinh học tạo thành bởi hỗn hợp các chủng này khi không có chất mang biochar trấu và khi có chất mang biochar trấu lần lượt là 78 và 98 %. Còn sử dụng chất mang biochar trấu không có vi sinh vật làm đối chứng thì thu được hiệu suất hấp phụ DO là 23 %. Như vậy, kết quả này mở ra tiềm năng ứng dụng biochar trấu làm chất mang cho các chủng vi khuẩn tạo màng sinh học để nâng cao hiệu quả xử lý ô nhiễm dầu.

Isolation of Petroleum Hydrocarbon Degrading Bacteria from the Liaohe Estuary at Cold Climate

2015 ◽  
Vol 1092-1093 ◽  
pp. 878-881
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Diesel Oil ◽  
Cold Climate ◽  
Hydrocarbon Degradation ◽  
Oil Degradation ◽  
Degrading Bacteria ◽  
Cold Tolerant ◽  
Diesel Oil Degradation

Two cold-tolerant petroleum hydrocarbon degrading bacteria strain named CHD1 and CHD2 were isolated from oil-contaminated soil at cold climate. The isolated strains were able use diesel oil as sole carbon. The petroleum hydrocarbon degradation rate was analyzed using UV-spectrometry-based methods. The results showed that the diesel oil degradation rate of CHD1 and CHD2 were 22% and 25%, respectively.

scholarly journals Assessment of diesel oil degradation by fungi

2020 ◽  
Author(s):  
Vivyan Pinheiro Simão Ribeiro ◽  
Glória Andreia Ferreira Hernández ◽  
Luana Pinto de Souza Tavares ◽  
Vicente Mussi-Dias ◽  
Maria das Graças Machado Freire
Keyword(s):  
Diesel Oil ◽  
Oil Degradation ◽  
Diesel Oil Degradation

A Ternary Photocatalyst with Double Heterojunctionsfor Efficient Diesel Oil Degradation

2021 ◽  
Vol 6 (13) ◽  
pp. 3117-3125
Author(s):  
Er‐Peng Bao ◽  
Songtao Long ◽  
Shuoqing Zhang ◽  
Huan Li ◽  
Weiguo Zhang ◽  
...  
Keyword(s):  
Diesel Oil ◽  
Oil Degradation ◽  
Diesel Oil Degradation

scholarly journals Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation

2010 ◽  
Vol 34 (3) ◽  
pp. 773-781 ◽  
Author(s):  
Douglas Antonio Alvaredo Paixão ◽  
Mauricio Rocha Dimitrov ◽  
Rodrigo Matheus Pereira ◽  
Fábio Raphael Accorsini ◽  
Maria Benincasa Vidotti ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Diesel Oil ◽  
Molecular Techniques ◽  
Rrna Gene ◽  
Oil Degradation ◽  
Partial Sequencing ◽  
Oligonucleotide Primers ◽  
The 16S Rrna Gene ◽  
Diesel Oil Degradation

Diesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was amplified by PCR reaction using specific oligonucleotide primers for the 16S rRNA gene. The PCR products were cloned into a pGEM-T-Easy vector (Promega), and Escherichia coli was used as the host cell for recombinant DNAs. The partial clone sequencing was obtained using universal oligonucleotide primers from the vector. The genetic library obtained generated 431 clones. All the sequenced clones presented similarity to phylum Proteobacteria, with Gammaproteobacteria the most present group (49.8 % of the clones), followed by Alphaproteobacteira (44.8 %) and Betaproteobacteria (5.4 %). The Pseudomonas genus was the most abundant in the metagenomic library, followed by the Parvibaculum and the Sphingobium genus, respectively. After partial sequencing of the 16S rRNA, the diversity of the bacterial consortium was estimated using DOTUR software. When comparing these sequences to the database from the National Center for Biotechnology Information (NCBI), a strong correlation was found between the data generated by the software used and the data deposited in NCBI.

Optimization of nutrient component for diesel oil degradation by Rhodococcus erythropolis

2008 ◽  
Vol 56 (10) ◽  
pp. 1714-1718 ◽  
Author(s):  
Lei Huang ◽  
Ting Ma ◽  
Dan Li ◽  
Feng-lai Liang ◽  
Ru-Lin Liu ◽  
...  
Keyword(s):  
Rhodococcus Erythropolis ◽  
Diesel Oil ◽  
Oil Degradation ◽  
Diesel Oil Degradation
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