Trace levels of the CHO host cell protease cathepsin D caused particle formation in a monoclonal antibody product

2015 ◽  
Vol 31 (5) ◽  
pp. 1360-1369 ◽  
Author(s):  
Jared S. Bee ◽  
Liu Tie ◽  
Douglas Johnson ◽  
Mariana N. Dimitrova ◽  
Kristin C. Jusino ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Cathepsin D ◽  
Particle Formation ◽  
Monoclonal Antibody Product

Production of pharmaceutical-grade recombinant aprotinin and a monoclonal antibody product using plant-based transient expression systems

2010 ◽  
Vol 8 (5) ◽  
pp. 638-654 ◽  
Author(s):  
Gregory P. Pogue ◽  
Fakhrieh Vojdani ◽  
Kenneth E. Palmer ◽  
Ernie Hiatt ◽  
Steve Hume ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Transient Expression ◽  
Expression Systems ◽  
Monoclonal Antibody Product

Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites

2010 ◽  
Vol 55 (4) ◽  
Author(s):  
Adriana Aguado-Martínez ◽  
Gema Álvarez-García ◽  
Gereon Schares ◽  
Verónica Risco-Castillo ◽  
Aurora Fernández-García ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Cell Invasion ◽  
Neospora Caninum ◽  
Parasitophorous Vacuole ◽  
Membrane Surface ◽  
Chronic Phase ◽  
Host Cell Invasion ◽  
Invasion Mechanisms ◽  
The Matrix

AbstractNeospora caninum negatively impacts bovine reproductive performance around the world. Addressing this problem requires a greater understanding of the parasite’s molecular biology. In this study, monoclonal antibodies against recombinant proteins were successfully developed and employed to characterise two different proteins of N. caninum: the acute phase-associated NcGRA7 and the chronic phase-associated NcSAG4. Immunofluorescence with the anti-rNcGRA7 monoclonal antibody suggested that NcGRA7 trafficks from tachyzoite dense granules to the matrix of the parasitophorous vacuole and parasite’s surroundings. Furthermore, NcGRA7 is also expressed in the bradyzoite stage and localised on the matrix of bradyzoite-positive vacuoles. NcGRA7 appears to be partially involved in the tachyzoite-invasion mechanisms, as an anti-rNcGRA7 monoclonal antibody partially inhibited in vitro tachyzoite-invasion. A monoclonal antibody specific for NcSAG4 confirmed this protein’s bradyzoitespecific expression both by western blot and immunofluorescence. However, some bradyzoite-positive vacuoles only weakly expressed NcSAG4, if it was expressed at all. The specificity of the anti-rNcSAG4 monoclonal antibody was confirmed by the recognition of the NcSAG4 in the membrane surface of Nc-1SAG4c transgenic tachyzoites, which constitutively expresses NcSAG4. Blocking NcSAG4 of Nc-1SAG4c tachyzoites with the monoclonal antibody did not affect host cell invasion. However, its implication on the host cell adhesion or host immune evasion should not be discarded.

Gelation of a Monoclonal Antibody at the Silicone Oil–Water Interface and Subsequent Rupture of the Interfacial Gel Results in Aggregation and Particle Formation

2015 ◽  
Vol 104 (4) ◽  
pp. 1282-1290 ◽  
Author(s):  
Shyam B. Mehta ◽  
Rachael Lewus ◽  
Jared S. Bee ◽  
Theodore W. Randolph ◽  
John F. Carpenter
Keyword(s):  
Monoclonal Antibody ◽  
Silicone Oil ◽  
Particle Formation ◽  
Water Interface ◽  
Oil Water

Characterization of Subvisible Particle Formation During the Filling Pump Operation of a Monoclonal Antibody Solution

2011 ◽  
Vol 100 (10) ◽  
pp. 4198-4204 ◽  
Author(s):  
Arpan Nayak ◽  
James Colandene ◽  
Victor Bradford ◽  
Melissa Perkins
Keyword(s):  
Monoclonal Antibody ◽  
Particle Formation ◽  
Pump Operation ◽  
Antibody Solution

A Monoclonal Antibody to Alpha Tubulin Recognizes Host Cell andTrypanosoma cruziTubulins1

1988 ◽  
Vol 35 (1) ◽  
pp. 123-129 ◽  
Author(s):  
JEROME J. PAULIN ◽  
CHARLES H. KEITH ◽  
RICK L. TARLETON
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Alpha Tubulin

A rapid cIEF–ESI–MS/MS method for host cell protein analysis of a recombinant human monoclonal antibody

Talanta ◽  
2012 ◽  
Vol 98 ◽  
pp. 253-256 ◽  
Author(s):  
Guijie Zhu ◽  
Liangliang Sun ◽  
Roza Wojcik ◽  
Dawn Kernaghan ◽  
James B. McGivney ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Host Cell ◽  
Human Monoclonal Antibody ◽  
Protein Analysis ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Esi Ms
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