Simple micropatterning method for enhancing fusion efficiency and responsiveness to electrical stimulation of C2C12 myotubes

2014 ◽  
Vol 31 (1) ◽  
pp. 220-225 ◽  
Author(s):  
Yuzo Takayama ◽  
Akira Wagatsuma ◽  
Takayuki Hoshino ◽  
Kunihiko Mabuchi
Keyword(s):  
Electrical Stimulation ◽  
C2c12 Myotubes ◽  
Fusion Efficiency ◽  
Stimulation Of

scholarly journals Electrical stimulation of C2C12 myotubes induces contractions and represses thyroid-hormone-dependent transcription of the fast-type sarcoplasmic-reticulum Ca2+-ATPase gene

1997 ◽  
Vol 321 (3) ◽  
pp. 845-848 ◽  
Author(s):  
Marc H. M. THELEN ◽  
Warner S. SIMONIDES ◽  
Cornelis van HARDEVELD
Keyword(s):  
Electrical Stimulation ◽  
Thyroid Hormone ◽  
Sarcoplasmic Reticulum ◽  
Contractile Activity ◽  
Motor Nerve ◽  
C2c12 Myotubes ◽  
Transcriptional Level ◽  
Regulation Of Expression ◽  
Atpase Gene ◽  
Stimulation Of

Chronic low-frequency contraction of skeletal muscle, either induced by a slow motor nerve or through direct electrical stimulation, generally induces expression of proteins associated with the slow phenotype, while repressing the corresponding fast isoforms. Contractions thereby counteract the primarily transcriptional effect of thyroid hormone (T3), which results in the selective induction and stimulation of expression of fast isoforms. We studied the regulation of expression of the fast-type sarcoplasmic-reticulum Ca2+-ATPase (SERCA1), a characteristic component of the fast phenotype. Previous work suggested that reduction of SERCA1 expression by contractile activity might result from interference with the T3-dependent transcriptional stimulation of the SERCA1 gene. The present study was set up to test this unexpected mode of action of contractile activity. We show that electrical stimulation of C2C12 mouse myotubes, which results in synchronous contractions at the imposed frequency, reduces basal but virtually abolishes T3-dependent SERCA1 expression. T3-dependent expression of a reporter gene driven by the SERCA1 promoter was similarly affected by electrical stimulation. This is the first demonstration that the counteracting effects on muscle gene expression of electrically induced contractions and T3 may interact at the transcriptional level.

ADENOHYPOPHYSEAL LH CONTENT IN NORMAL, ANDROGEN-STERILIZED AND PROGESTERONE-PRIMED STERILE FEMALE RATS

1962 ◽  
Vol 39 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Roger A. Gorski ◽  
Charles A. Barraclough
Keyword(s):  
Ascorbic Acid ◽  
Electrical Stimulation ◽  
Quantitative Determination ◽  
Median Eminence ◽  
Hypothalamic Stimulation ◽  
Female Rats ◽  
Rat Pituitary ◽  
Gland Content ◽  
Stimulation Of

ABSTRACT We have previously suggested that the failure of the androgen-sterilized, persistent-oestrous rat to ovulate, following electrical stimulation of the median eminence structures of the hypothalamus, is due to an insufficiency in adenohypophyseal LH concentration. Using the ovarian ascorbic acid technique for quantitative determination of pituitary LH content, the present studies have demonstrated that the sterile rat pituitary gland contains one-third the LH content of the normal prooestrous gland. Furthermore, not only does progesterone priming of this persistent-oestrous rat result in a 75 % increase in LH concentration, but on hypothalamic stimulation sufficient LH is released to induce ovulation. The decrease in LH concentration which accompanies ovulation in the progesterone-primed, sterile rat is approximately 45 % of the total gland content as compared with a 51 % decrease in pituitary content in the normal cyclic rat.

Sign in / Sign up

Export Citation Format

Share Document