Cell culture media supplementation of uncommonly used sugars sucrose and tagatose for the targeted shifting of protein glycosylation profiles of recombinant protein therapeutics

2014 ◽  
Vol 30 (6) ◽  
pp. 1419-1431 ◽  
Author(s):  
Patrick Hossler ◽  
Sean McDermott ◽  
Christopher Racicot ◽  
Christopher Chumsae ◽  
Haly Raharimampionona ◽  
...  
Keyword(s):  
Cell Culture ◽  
Recombinant Protein ◽  
Culture Media ◽  
Protein Therapeutics ◽  
Protein Glycosylation ◽  
Cell Culture Media ◽  
Recombinant Protein Therapeutics ◽  
Media Supplementation

Cell culture media supplementation of infrequently used sugars for the targeted shifting of protein glycosylation profiles

2017 ◽  
Vol 33 (2) ◽  
pp. 511-522 ◽  
Author(s):  
Patrick Hossler ◽  
Christopher Racicot ◽  
Christopher Chumsae ◽  
Sean McDermott ◽  
Keith Cochran
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Protein Glycosylation ◽  
Cell Culture Media ◽  
Media Supplementation

scholarly journals Conversion of spent CHO cell culture media waste to new fermentation feed efficiently supports production of recombinant protein by Escherichia coli

2021 ◽  
Author(s):  
Ciara Lynch ◽  
David J O'Connell
Keyword(s):  
Cell Culture ◽  
Recombinant Protein ◽  
Culture Media ◽  
Mass Spectrometry Analysis ◽  
Microbial Fermentation ◽  
Differential Protein Expression ◽  
Cell Culture Media ◽  
Spectrometry Analysis ◽  
E Coli ◽  
Rich Media

Deriving new value from waste streams is a central aim of the circular bioeconomy. In this study we investigate whether chemically defined spent media (CDSM) waste from cell culture bioprocess can be effectively recycled and used as a feed in microbial fermentation to produce new recombinant protein products. Our results show that 1) CDSM supplemented with 2% glycerol supported a specific growth rate of E. coli cultures equivalent to that achieved using a nutritionally rich media (LB) used as a baseline reference. 2) The amount of recombinant protein produced following induction in an expression screen was approximately two-fold higher in the CDSM fed cultures than that of baseline. 3) Mass spectrometry analysis of the proteome of E. coli cultures fed in CDSM revealed a greater or lesser differential protein expression pattern depending on supplementation conditions. Further, in a 16 hr fermentation the optimised CDSM-fed culture delivered a protein yield of more than double that achieved by the baseline media. We conclude that spent cell culture media, which represents millions of litres of waste generated by the bioprocessing industry annually, has the potential to be a valuable feed resource for the production of recombinant proteins in secondary microbial fermentation.

Cell culture media supplementation of bioflavonoids for the targeted reduction of acidic species charge variants on recombinant therapeutic proteins

2015 ◽  
Vol 31 (4) ◽  
pp. 1039-1052 ◽  
Author(s):  
Patrick Hossler ◽  
Min Wang ◽  
Sean McDermott ◽  
Christopher Racicot ◽  
Kofi Chemfe ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Therapeutic Proteins ◽  
Cell Culture Media ◽  
Charge Variants ◽  
Media Supplementation

Cell culture media: NMR approaches to evaluating quality and nutrient consumption

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
KB Killday ◽  
AS Freund ◽  
C Fischer ◽  
KL Colson
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Cell Culture Media ◽  
Nutrient Consumption

Selection and preliminary application of DNA aptamer targeting A549 excreta in cell culture media

2021 ◽  
pp. 106811
Author(s):  
Yuanbin Guo ◽  
Ming Shi ◽  
Xiujuan Liu ◽  
Huagang Liang ◽  
Liming Gao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Dna Aptamer ◽  
Cell Culture Media ◽  
Preliminary Application

scholarly journals Benchmarking of commercially available CHO cell culture media for antibody production

2015 ◽  
Vol 99 (11) ◽  
pp. 4645-4657 ◽  
Author(s):  
David Reinhart ◽  
Lukas Damjanovic ◽  
Christian Kaisermayer ◽  
Renate Kunert
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

scholarly journals Intestinal and Hepatic Uptake of Dietary Peroxidized Lipids and Their Decomposition Products, and Their Subsequent Effects on Apolipoprotein A1 and Paraoxonase1

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1258
Author(s):  
Xueting Jiang ◽  
Pragney Deme ◽  
Rajat Gupta ◽  
Dmitry Litvinov ◽  
Kathryn Burge ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Degradation Products ◽  
Apolipoprotein A1 ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Atherosclerotic Cardiovascular Disease ◽  
Metabolic Fate ◽  
Cell Culture Media ◽  
Decomposition Products

Both pro- and antiatherosclerotic effects have been ascribed to dietary peroxidized lipids. Confusion on the role of peroxidized lipids in atherosclerotic cardiovascular disease is punctuated by a lack of understanding regarding the metabolic fate and potential physiological effects of dietary peroxidized lipids and their decomposition products. This study sought to determine the metabolic fate and physiological ramifications of 13-hydroperoxyoctadecadienoic acid (13-HPODE) and 13-HODE (13-hydroxyoctadecadienoic acid) supplementation in intestinal and hepatic cell lines, as well as any effects resulting from 13-HPODE or 13-HODE degradation products. In the presence of Caco-2 cells, 13-HPODE was rapidly reduced to 13-HODE. Upon entering the cell, 13-HODE appears to undergo decomposition, followed by esterification. Moreover, 13-HPODE undergoes autodecomposition to produce aldehydes such as 9-oxononanoic acid (9-ONA). Results indicate that 9-ONA was oxidized to azelaic acid (AzA) rapidly in cell culture media, but AzA was poorly absorbed by intestinal cells and remained detectable in cell culture media for up to 18 h. An increased apolipoprotein A1 (ApoA1) secretion was observed in Caco-2 cells in the presence of 13-HPODE, 9-ONA, and AzA, whereas such induction was not observed in HepG2 cells. However, 13-HPODE treatments suppressed paraoxonase 1 (PON1) activity, suggesting the induction of ApoA1 secretion by 13-HPODE may not represent functional high-density lipoprotein (HDL) capable of reducing oxidative stress. Alternatively, AzA induced both ApoA1 secretion and PON1 activity while suppressing ApoB secretion in differentiated Caco-2 cells but not in HepG2. These results suggest oxidation of 9-ONA to AzA might be an important phenomenon, resulting in the accumulation of potentially beneficial dietary peroxidized lipid-derived aldehydes.

Characterization of the impact of classical cell‐culture media on the response of electrochemical sensors

2021 ◽  
Author(s):  
Ayman Chmayssem ◽  
Lauriane Petit ◽  
Nicolas Verplanck ◽  
Véronique Mourier ◽  
Séverine Vignoud ◽  
...  
Keyword(s):  
Cell Culture ◽  
Electrochemical Sensors ◽  
Culture Media ◽  
Cell Culture Media ◽  
The Impact
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