The role of endoglucanase and endoxylanase in liquefaction of hydrothermally pretreated wheat straw

2014 ◽  
Vol 30 (4) ◽  
pp. 923-931 ◽  
Author(s):  
Pernille Anastasia Skovgaard ◽  
Lisbeth Garbrecht Thygesen ◽  
Henning Jørgensen ◽  
Maria Cardona ◽  
Emilio Tozzi ◽  
...  
2014 ◽  
Vol 4 (8) ◽  
pp. 2251-2259 ◽  
Author(s):  
Lucy Heap ◽  
Anthony Green ◽  
David Brown ◽  
Bart van Dongen ◽  
Nicholas Turner

The saccharification of wheat straw was improved when an incubation step was performed withTrametes versicolorlaccase (TvL) and the mediator 1-hydroxybenzotriazole (1-HBT) prior to an alkaline peroxide extraction (APE).


2013 ◽  
Vol 164 (1) ◽  
pp. 50-58 ◽  
Author(s):  
Borbála Erdei ◽  
Balázs Frankó ◽  
Mats Galbe ◽  
Guido Zacchi

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
María García-Torreiro ◽  
Miguel Álvarez Pallín ◽  
María López-Abelairas ◽  
Thelmo A. Lu-Chau ◽  
Juan M. Lema

AbstractBioconversion of lignocellulosic materials into ethanol requires an intermediate pretreatment step for conditioning biomass. Sugar yields from wheat straw were previously improved by the addition of a mild alkali pretreatment step before bioconversion by the white-rot fungus Irpex lacteus. In this work, an alternative alkaline treatment, which significantly reduces water consumption, was implemented and optimized. Sugar recovery increased 117% with respect to the previously developed alkaline wash process at optimal process conditions (30°C, 30 minutes and 35.7% (w/w) of NaOH). In order to further reduce operational costs, a system for alkali recycling was implemented. This resulted in the treatment of 150% more wheat straw using the same amount of NaOH. Finally, enzymatic hydrolysis was optimized and resulted in a reduction of enzyme dose of 33%.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chen Wang ◽  
Xuehong Ma ◽  
Gang Wang ◽  
Guitong Li ◽  
Kun Zhu

AbstractSoil O2 dynamics have significant influences on greenhouse gas emissions during soil management practice. In this study, we deployed O2-specific planar optodes to visualize spatiotemporal distribution of O2 in soils treated with biological soil disinfestation (BSD). This study aimed to reveal the role of anoxia development on emissions of N2O and CH4 from soil amended with crop residues during BSD period. The incorporation of crop residues includes wheat straw only, wheat straw with biochar and early straw incorporation. The anoxia in soil developed very fast within 3 days, while the O2 in headspace decreased much slower and it became anaerobic after 5 days, which was significantly affected by straw and biochar additions. The N2O emissions were positively correlated with soil hypoxic fraction. The CH4 emissions were not significant until the anoxia dominated in both soil and headspace. The co-application of biochar with straw delayed the anoxia development and extended the hypoxic area in soil, resulting in lower emissions of N2O and CH4. Those results highlight that the soil O2 dynamic was the key variable triggering the N2O and CH4 productions. Therefore, detailed information of soil O2 availability could be highly beneficial for optimizing the strategies of organic amendments incorporation in the BSD technique.


2018 ◽  
Vol 115 ◽  
pp. 139-148 ◽  
Author(s):  
Yiqing Yao ◽  
Andre David Bergeron ◽  
Maryam Davaritouchaee

2017 ◽  
Vol 184 (1) ◽  
pp. 48-62 ◽  
Author(s):  
Ramkumar B. Nair ◽  
Maryam M. Kabir ◽  
Patrik R. Lennartsson ◽  
Mohammad J. Taherzadeh ◽  
Ilona Sárvári Horváth

AbstractIntegration of wheat straw for a biorefinery-based energy generation process by producing ethanol and biogas together with the production of high-protein fungal biomass (suitable for feed application) was the main focus of the present study. An edible ascomycete fungal strain Neurospora intermedia was used for the ethanol fermentation and subsequent biomass production from dilute phosphoric acid (0.7 to 1.2% w/v) pretreated wheat straw. At optimum pretreatment conditions, an ethanol yield of 84 to 90% of the theoretical maximum, based on glucan content of substrate straw, was observed from fungal fermentation post the enzymatic hydrolysis process. The biogas production from the pretreated straw slurry showed an improved methane yield potential up to 162% increase, as compared to that of the untreated straw. Additional biogas production, using the syrup, a waste stream obtained post the ethanol fermentation, resulted in a combined total energy output of 15.8 MJ/kg wheat straw. Moreover, using thin stillage (a waste stream from the first-generation wheat-based ethanol process) as a co-substrate to the biogas process resulted in an additional increase by about 14 to 27% in the total energy output as compared to using only wheat straw-based substrates.


2010 ◽  
Vol 85 (9) ◽  
pp. 1291-1297 ◽  
Author(s):  
Pablo Alvira ◽  
María José Negro ◽  
Felicia Sáez ◽  
Mercedes Ballesteros

2020 ◽  
Vol 10 (21) ◽  
pp. 7638
Author(s):  
Vasile-Florin Ursachi ◽  
Gheorghe Gutt

The aim of this study is to find the optimal pretreatment conditions and hydrolysis in order to obtain a high yield of bioethanol from wheat straw. The pretreatments were performed with different concentrations of sulphuric acid 1, 2 and 3% (v/v), and were followed by an enzymatic hydrolysis that was performed by varying the solid-to-liquid ratio (1/20, 1/25 and 1/30 g/mL) and the enzyme dose (30/30 µL/g, 60/60 µL/g and 90/90 µL/g Viscozyme® L/Celluclast® 1.5 L). This mix of enzymes was used for the first time in the hydrolysis process of wheat straws which was previously pretreated with dilute sulfuric acid. Scanning electron microscopy indicated significant differences in the structural composition of the samples because of the pretreatment with H2SO4 at different concentrations, and ATR-FTIR analysis highlighted the changes in the chemical composition in the pretreated wheat straw as compared to the untreated one. HPLC-RID was used to identify and quantify the carbohydrates content resulted from enzymatic hydrolysis to evaluate the potential of using wheat straws as a raw material for production of cellulosic ethanol in Romania. The highest degradation of lignocellulosic material was obtained in the case of pretreatment with 3% H2SO4 (v/v), a solid-to-liquid ratio of 1/30 and an enzyme dose of 90/90 µL/g. Simultaneous saccharification and fermentation were performed using Saccharomyces cerevisiae yeast, and for monitoring the fermentation process a BlueSens equipment was used provided with ethanol, O2 and CO2 cap sensors mounted on the fermentation flasks. The highest concentration of bioethanol was obtained after 48 h of fermentation and it reached 1.20% (v/v).


2006 ◽  
Vol 130 (1-3) ◽  
pp. 496-508 ◽  
Author(s):  
Ignacio Ballesteros ◽  
Ma José Negro ◽  
Jose Miguel Oliva ◽  
Araceli Cabanas ◽  
Paloma Manzanares ◽  
...  

2016 ◽  
Vol 7 (6) ◽  
pp. 1389-1396 ◽  
Author(s):  
Muhammad Irfan ◽  
Umar Asghar ◽  
Muhammad Nadeem ◽  
Rubina Nelofer ◽  
Quratulain Syed ◽  
...  

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