Different types of aqueous two-phase systems for biomolecule and bioparticle extraction and purification

2013 ◽  
Vol 29 (6) ◽  
pp. 1343-1353 ◽  
Author(s):  
João Vitor Dutra Molino ◽  
Daniela de Araújo Viana Marques ◽  
Adalberto Pessoa Júnior ◽  
Priscila Gava Mazzola ◽  
Maria Silvia Viccari Gatti
Keyword(s):  
Two Phase ◽  
Extraction And Purification ◽  
Different Types ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Purification of microbial b-galactosidase from Kluyveromyces fragilis by bioaffinity partitioning

1999 ◽  
Vol 30 (4) ◽  
pp. 324-331 ◽  
Author(s):  
Maria Estela da Silva ◽  
Telma Teixeira Franco
Keyword(s):  
Partition Coefficient ◽  
Second Step ◽  
Step Method ◽  
Kluyveromyces Fragilis ◽  
Two Phase ◽  
Rich Phase ◽  
Extraction And Purification ◽  
Peg 4000 ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

This work investigated the partitioning of b-galactosidase from Kluyveromyces fragilis in aqueous two-phase systems (ATPS) by bioaffinity. PEG 4000 was chemically activated with thresyl chloride, and the biospecific ligand p-aminophenyl 1-thio-b-D-galactopyranoside (APGP) was attached to the activated PEG 4000. A new two-step method for extraction and purification of the enzyme b-galactosidase from Kluyveromyces fragilis was developed. In the first step, a system composed of 6% PEG 4000-APGP and 8% dextran 505 was used, where b-galactosidase was strongly partitioned to the top phase (K = 2,330). In the second step, a system formed of 13% PEG-APGP and 9% phosphate salt was used to revert the value of the partition coefficient of b-galactosidase (K = 2 x 10-5) in order to provide the purification and recovery of 39% of the enzyme in the bottom salt-rich phase.

scholarly journals Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two-Phase Systems Strategies

2008 ◽  
Vol 31 (6) ◽  
pp. 838-845 ◽  
Author(s):  
J. Benavides ◽  
O. Aguilar ◽  
B. H. Lapizco-Encinas ◽  
M. Rito-Palomares
Keyword(s):  
Two Phase ◽  
Extraction And Purification ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Integrated process of extraction and purification of betanin from Opuntia ficus-indica using aqueous two-phase systems based on THF and sodium salts

2017 ◽  
Vol 53 (5) ◽  
pp. 734-744 ◽  
Author(s):  
Ricardo P. Santos ◽  
Luana M. Souza ◽  
Acenini L. Balieiro ◽  
Cleide M. F. Soares ◽  
Álvaro S. Lima ◽  
...  
Keyword(s):  
Integrated Process ◽  
Two Phase ◽  
Opuntia Ficus Indica ◽  
Sodium Salts ◽  
Extraction And Purification ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

2014 ◽  
Vol 52 (5) ◽  
pp. 2878-2885 ◽  
Author(s):  
Bin Jiang ◽  
Zhibiao Feng ◽  
Chunhong Liu ◽  
Yingcao Xu ◽  
Dongmei Li ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Two Phase ◽  
Extraction And Purification ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Optimized and Scalable Green Extraction of Pristimerin, an Anticancerigen from Mortonia greggii, by Ethanol–Phosphate Aqueous Two-Phase Systems

2021 ◽  
Vol 60 (15) ◽  
pp. 5403-5410
Author(s):  
Martha Alicia González-Félix ◽  
Luis Alberto Mejía-Manzano ◽  
Bertha A. Barba-Dávila ◽  
Sergio O. Serna-Saldívar ◽  
José González-Valdez
Keyword(s):  
Two Phase ◽  
Green Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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