A protocol to remove colored metabolites and other inhibitors from plant tissues to facilitate RNA isolation suitable for downstream applications

2012 ◽  
Vol 28 (5) ◽  
pp. 1303-1307 ◽  
Author(s):  
Ravi S. Singh ◽  
Sanjay Kumar
Keyword(s):  
Rna Isolation ◽  
Plant Tissues

scholarly journals An RNA isolation system for plant tissues rich in secondary metabolites

2011 ◽  
Vol 4 (1) ◽  
Author(s):  
Sanjay Ghawana ◽  
Asosii Paul ◽  
Hitesh Kumar ◽  
Arun Kumar ◽  
Harsharan Singh ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Rna Isolation ◽  
Plant Tissues ◽  
Isolation System

scholarly journals A Simple and Rapid System for DNA and RNA Isolation from Diverse Plants Using Handmade Kit

2019 ◽  
Author(s):  
Farhad Masoomi-Aladizgeh ◽  
Leila Jabbari ◽  
Reza Khayam Nekouei ◽  
Ali Aalami
Keyword(s):  
Nucleic Acid ◽  
Extraction Method ◽  
Ethylenediaminetetraacetic Acid ◽  
Rna Extraction ◽  
Rna Isolation ◽  
Plant Tissues ◽  
Hexadecyltrimethylammonium Bromide ◽  
Dna And Rna ◽  
Extraction Buffer ◽  
Main Components

Abstract This protocol describes a rapid DNA and RNA extraction method for plant tissues. Hexadecyltrimethylammonium bromide (CTAB), sodium chloride (NaCl), tris base, and ethylenediaminetetraacetic acid (EDTA) are the main components of the extraction buffer. In contrast to all previously reported protocols, this extraction method does not require any stock solutions. This isolation buffer is potential of extracting both DNA and RNA simultaneously. Depending on the purpose of the project, the corresponding steps can be slightly altered to obtain either DNA or RNA. The big advantage of this method is to use general laboratory chemicals to make a powerful extraction buffer, resulting in high quality and quantity nucleic acid. Also, CTAB in this buffer is capable of isolating nucleic acid from recalcitrant plants enriched in secondary metabolites. Importantly, this method is recommended for the projects at which isolating nucleic acid in a short time is of crucial importance. This method probably is usable for all plant tissues and takes about an hour.

RNA isolation from plant tissues rich in polysaccharides

2003 ◽  
Vol 314 (2) ◽  
pp. 319-321 ◽  
Author(s):  
Arun Dev Sharma ◽  
Prabhjot Kaur Gill ◽  
Prabhjeet Singh
Keyword(s):  
Rna Isolation ◽  
Plant Tissues

Microscopy studies of powdery mildew on summer squash

1991 ◽  
Vol 49 ◽  
pp. 520-521
Author(s):  
John S. Gardner ◽  
W. M. Hess
Keyword(s):  
Powdery Mildew ◽  
Tip Growth ◽  
Hyphal Growth ◽  
Plant Tissues ◽  
Vegetative Hypha ◽  
Powdery Mildews ◽  
Summer Squash ◽  
Conidial Formation ◽  
Polar Tip Growth ◽  
Short Conidiophore

Powdery mildews are characterized by the appearance of spots or patches of a white to grayish, powdery, mildewy growth on plant tissues, entire leaves or other organs. Ervsiphe cichoracearum, the powdery mildew of cucurbits is among the most serious parasites, and the most common. The conidia are formed similar to the process described for Ervsiphe graminis by Cole and Samson. Theconidial chains mature basipetally from a short, conidiophore mother-cell at the base of the fertile hypha which arises holoblastically from the conidiophore. During early development it probably elongates by polar-tip growth like a vegetative hypha. A septum forms just above the conidiophore apex. Additional septa develop in acropetal succession. However, the conidia of E. cichoracearum are more doliform than condia from E. graminis. The purpose of these investigations was to use scanning electron microscopy (SEM) to demonstrate the nature of hyphal growth and conidial formation of E. cichoracearum on field-grown squash leaves.

Localization of acid phosphatases in root cap of rice plant

1991 ◽  
Vol 49 ◽  
pp. 224-225
Author(s):  
Y. R. Chen ◽  
Y. F. Huang ◽  
W. S. Chen
Keyword(s):  
Rice Plant ◽  
Developmental Stages ◽  
Uranyl Acetate ◽  
Root Cap ◽  
Plant Tissues ◽  
Acid Phosphatases ◽  
Root Tips ◽  
Buffer Solution ◽  
Cacodylate Buffer ◽  
Ethanol Series

Acid phosphatases are widely distributed in different tisssues of various plants. Studies on subcellular localization of acid phosphatases show they might be present in cell wall, plasma lemma, mitochondria, plastid, vacuole and nucleus. However, their localization in rice cell varies with developmental stages of cells and plant tissues. In present study, acid phosphatases occurring in root cap are examined.Sliced root tips of ten-day-old rice(Oryza sativa) seedlings were fixed in 0.1M cacodylate buffer containing 2.5% glutaraldehyde for 2h, washed overnight in same buffer solution, incubated in Gomori's solution at 37° C for 90min, post-fixed in OsO4, dehydrated in ethanol series and finally embeded in Spurr's resin. Sections were doubly stained with uranyl acetate and lead citrate, and observed under Hitachi H-600 at 75 KV.

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