scholarly journals Development of a stem cell spheroid‐laden patch with high retention at skin wound site

2021 ◽  
Author(s):  
Gun‐Jae Jeong ◽  
Gwang‐Bum Im ◽  
Tae‐Jin Lee ◽  
Sung‐Won Kim ◽  
Hye Ran Jeon ◽  
...  
Keyword(s):  
Stem Cell ◽  
Skin Wound ◽  
Wound Site ◽  
Cell Spheroid

scholarly journals Sundew-Inspired Adhesive Hydrogel Threads through Reversible Complexation of Polyphenol and Boronic Acid

2021 ◽  
Vol 11 (18) ◽  
pp. 8591
Author(s):  
Jae Hyuk Choi ◽  
Donghee Son ◽  
Mikyung Shin
Keyword(s):  
Boronic Acid ◽  
Skin Wound ◽  
Body Fluids ◽  
The Body ◽  
Wound Dressings ◽  
Suture Materials ◽  
Wound Site ◽  
Long Term Stability ◽  
State Dependent

Adhesive hydrogels have been utilized as tissue sealants, hemostatic agents, and wound dressings, with the aim of replacing conventional sutures. To prevent immune response and serious inflammation from those hydrogels after sealing, natural biocompatible polysaccharides are widely used as a component of the hydrogels. However, the weak mechanical strength, insufficient adhesiveness, and rapid dissociation of the hydrogels necessitates additional suturing at the wound site. In this study, we report on a solid polysaccharide thread reversibly crosslinked with boronic acid-polyphenol complexation and its adhesive gelation for complete tissue sealing without additional suturing. The polysaccharide adhesive suture threads demonstrated long-term stability, which is useful when used for skin wound suturing. Specifically, their robust adhesion property occurred as soon as the threads were soaked, attaining a swollen hydrogel state, dependent on the presence of body fluids after suturing. Moreover, the pH of the body fluids affects the viscoelasticity and adhesiveness of the hydrogels in order to ensure a tight sealing. Therefore, we expect that these pH-responsive adhesive threads would be promising for the development of on-demand functional suture materials.

Enhancement of human adipose-derived stem cell spheroid differentiation in an in situ enzyme-crosslinked gelatin hydrogel

2019 ◽  
Vol 7 (7) ◽  
pp. 1064-1075 ◽  
Author(s):  
Ching-Cheng Tsai ◽  
Yu-Jun Hong ◽  
Randall J. Lee ◽  
Nai-Chen Cheng ◽  
Jiashing Yu
Keyword(s):  
Stem Cell ◽  
Cell Spheroid ◽  
Cell Spheroids ◽  
Gelatin Hydrogel ◽  
Adipose Derived Stem Cell

Standardized human adipose-derived stem cell spheroids can be harvested abundantly and the differentiation capability of cell spheroids performed well in the enzyme-crosslinked gelatin hydrogel.

scholarly journals Enzyme-Crosslinked Gelatin Hydrogel with Adipose-Derived Stem Cell Spheroid Facilitating Wound Repair in the Murine Burn Model

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2997
Author(s):  
Ting-Yu Lu ◽  
Kai-Fu Yu ◽  
Shuo-Hsiu Kuo ◽  
Nai-Chen Cheng ◽  
Er-Yuan Chuang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Three Dimensional ◽  
Stromal Vascular Fraction ◽  
Skin Wound ◽  
3D Scaffold ◽  
Skin Wound Healing ◽  
Cell Spheroid ◽  
Cell Spheroids ◽  
Adipose Derived Stem Cell

Engineered skin that can facilitate tissue repair has been a great advance in the field of wound healing. A well-designed dressing material together with active biological cues such as cells or growth factors can overcome the limitation of using auto-grafts from patients. Recently, many studies showed that human adipose-derived stem cells (hASCs) can be used to promote wound healing and skin tissue engineering. hASCs have already been widely applied for clinical trials. hASCs can be harvested abundantly because they can be easily isolated from fat tissue known as the stromal vascular fraction (SVF). On the other hand, increasing studies have proven that cells from spheroids can better simulate the biological microenvironment and can enhance the expression of stemness markers. However, a three-dimensional (3D) scaffold that can harbor implanted cells and can serve as a skin-repaired substitute still suffers from deficiency. In this study, we applied a gelatin/microbial transglutaminase (mTG) hydrogel to encapsulate hASC spheroids to evaluate the performance of 3D cells on skin wound healing. The results showed that the hydrogel is not toxic to the wound and that cell spheroids have significantly improved wound healing compared to cell suspension encapsulated in the hydrogel. Additionally, a hydrogel with cell spheroids was much more effective than other groups in angiogenesis since the cell spheroid has the possibility of cell–cell signaling to promote vascular generation.

Adipose tissue derived mesenchymal stem cell (AD-MSC) promotes skin wound healing in diabetic rats

2011 ◽  
Vol 93 (2) ◽  
pp. 228-234 ◽  
Author(s):  
Mohsen Khosravi Maharlooei ◽  
Mansooreh Bagheri ◽  
Zhabiz Solhjou ◽  
Behnam Moein Jahromi ◽  
Majid Akrami ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Skin Wound Healing

Stem cell spheroid engineering with osteoinductive and ROS scavenging nanofibers for bone regeneration

2020 ◽  
Author(s):  
Ha Yeon Byun ◽  
Gyu Nam Jang ◽  
Jinkyu Lee ◽  
Min-Ho Hong ◽  
Hyunjung Shin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Regeneration ◽  
Ros Scavenging ◽  
Cell Spheroid
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