scholarly journals Ventilation‐induced epithelial injury drives biological onset of lung trauma in vitro and is mitigated with prophylactic anti‐inflammatory therapeutics

2021 ◽  
Author(s):  
Eliram Nof ◽  
Arbel Artzy‐Schnirman ◽  
Saurabh Bhardwaj ◽  
Hadas Sabatan ◽  
Dan Waisman ◽  
...  
Keyword(s):  
Epithelial Injury ◽  
Lung Trauma ◽  
Anti Inflammatory

scholarly journals Ventilation-induced epithelial injury drives biological onset of lung trauma in vitro and is mitigated with anti-inflammatory therapeutics

2021 ◽  
Author(s):  
Eliram Nof ◽  
Arbel Artzy-Schnirman ◽  
Saurabh Bhardwaj ◽  
Hadas Sabatan ◽  
Dan Waisman ◽  
...  
Keyword(s):  
Upper Airway ◽  
Bronchial Epithelium ◽  
Mechanical Forces ◽  
Pulmonary Injury ◽  
Inflammatory Signaling ◽  
Epithelial Injury ◽  
Lung Trauma ◽  
Immunological Factors ◽  
Anti Inflammatory

Mortality rates among patients suffering from acute respiratory failure remain perplexingly high despite maintenance of blood homeostasis. The biotrauma hypothesis advances that mechanical forces from invasive ventilation trigger immunological factors that spread systemically. Yet, how these forces elicit an immune response remains unclear. Here we show that flow-induced stresses under mechanical ventilation can injure the bronchial epithelium of ventilated in vitro upper airway models and directly modulate inflammatory cytokine secretion associated with pulmonary injury. We identify site-specific susceptibility to epithelial erosion in airways from jet-flow impaction and measure an increase in cell apoptosis and modulated secretions of cytokines IL-6, 8 and 10. We find that prophylactic pharmacological treatment with anti-inflammatory therapeutics reduces apoptosis and pro-inflammatory signaling during ventilation. Our 3D in vitro airway platform points to a previously overlooked origin of lung injury and showcases translational opportunities in preclinical pulmonary research towards protective therapies and improved protocols for patient care.

scholarly journals Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model

2019 ◽  
Vol 11 (2) ◽  
pp. 84-99 ◽  
Author(s):  
Mariana Lizeth Orozco Morales ◽  
Nagi M Marsit ◽  
Owen D McIntosh ◽  
Andrew Hopkinson ◽  
Laura E Sidney
Keyword(s):  
Stem Cells ◽  
Corneal Stroma ◽  
Epithelial Injury ◽  
Corneal Epithelial ◽  
Injury Model ◽  
Anti Inflammatory

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory

In vitro anti-inflammatory and wound healing activities of Citrus auraptene

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
F Epifano ◽  
S Genovese ◽  
L Zhao ◽  
V Dang La ◽  
D Grenier
Keyword(s):  
Wound Healing ◽  
Anti Inflammatory

Evaluation of in vitro anti-inflammatory effects of the remaining water subextract of Cistus laurifolius leaves

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
İ Atay ◽  
AZ İlter ◽  
Y Bağatur ◽  
D Telci ◽  
H Kırmızıbekmez ◽  
...  
Keyword(s):  
Anti Inflammatory

Haemanthus coccineus extract shows profound anti-inflammatory actions in vitro and in vivo due to its main alkaloid narciclasine

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
S Fuchs ◽  
LT Hsieh ◽  
W Saarberg ◽  
CAJ Erdelmeier ◽  
TA Wichelhaus ◽  
...  
Keyword(s):  
Anti Inflammatory
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