scholarly journals The assembly of integrated rat intestinal‐hepatocyte cultures

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Anjaney Kothari ◽  
Padmavathy Rajagopalan
Keyword(s):  
Hepatocyte Cultures

Toxicological properties of thymoquinone in primary rat hepatocyte cultures

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
M Khader ◽  
N Bresgen ◽  
P Eckl
Keyword(s):  
Rat Hepatocyte ◽  
Hepatocyte Cultures

scholarly journals THE EXTRACELLULAR MATRIX CONTROL OF ACUTE PHASE RESPONSE IN RAT HEPATOCYTE CULTURES

Shock ◽  
1995 ◽  
Vol 4 (Supplement) ◽  
pp. 52
Author(s):  
E. Adoeva ◽  
T. Stepanova ◽  
I. Diakonov ◽  
A. Polevschlkov
Keyword(s):  
Extracellular Matrix ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Phase Response ◽  
Rat Hepatocyte ◽  
Hepatocyte Cultures ◽  
Matrix Control

scholarly journals Determinants of human apolipoprotein [a] secretion from mouse hepatocyte cultures

2001 ◽  
Vol 42 (1) ◽  
pp. 60-69 ◽  
Author(s):  
Jin Wang ◽  
Jennifer Boedeker ◽  
Helen H. Hobbs ◽  
Ann L. White
Keyword(s):  
Mouse Hepatocyte ◽  
Human Apolipoprotein ◽  
Hepatocyte Cultures ◽  
Apolipoprotein A

scholarly journals Endotoxin Stimulates Liver Macrophages To Release Mediators That Inhibit an Early Step in Hepadnavirus Replication

2000 ◽  
Vol 74 (12) ◽  
pp. 5525-5533 ◽  
Author(s):  
Uta Klöcker ◽  
Ursula Schultz ◽  
Heinz Schaller ◽  
Ulrike Protzer
Keyword(s):  
Fluorescent Protein ◽  
Virus Production ◽  
Antiviral Effect ◽  
Progeny Virus ◽  
Dna Templates ◽  
Liver Macrophages ◽  
Hepatocyte Cultures ◽  
Proinflammatory Mediators ◽  
Duck Hepatitis

ABSTRACT Hepadnaviruses are known to be sensitive to various extracellular mediators. Therefore, bacterial endotoxin, which induces the secretion of proinflammatory mediators in the liver, was studied for its effect on hepadnavirus infection in vitro using the duck hepatitis B virus (DHBV) model. In initial experiments, endotoxin was shown to inhibit DHBV replication in primary duck hepatocyte cultures prepared by standard collagenase perfusion. As a primary endotoxin target, hepatic nonparenchymal cells (NPC) contaminating primary hepatocyte cultures, and among these probably macrophages (Kupffer cells), were identified to secrete polypeptide mediators into the cell culture medium. When added during DHBV infection, these mediators elicited the principal antiviral effect in a dose-dependent fashion. On the molecular level, they inhibited accumulation of viral proteins as well as amplification of the nuclear extrachromosomal DHBV DNA templates. In hepatocytes with an established DHBV infection, DHBV protein and progeny virus production was inhibited while the levels of established nuclear DHBV DNA templates and viral transcripts remained unaffected. Finally, in hepatocytes infected with a replication-deficient recombinant DHBV-green fluorescent protein (GFP) virus, the endotoxin-induced mediators markedly reduced GFP expression from chimeric DHBV-GFP transcripts, indicating that the major effect is at a level of translation of viral RNAs. Taken together, the data obtained demonstrate that antiviral mediators, and among these the cytokines alpha interferon (IFN-α) and IFN-γ, are released from hepatic NPC, most probably liver macrophages, upon endotoxin stimulation; furthermore, these mediators act at a posttranscriptional step of hepadnavirus replication.

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