Localized electron polarization in a substrate analog binding to the active site of enoyl-CoA hydratase: Raman spectroscopic and conformational analyses of rotamers of hexadienoyl thiolesters

1995 ◽  
Vol 1 (6) ◽  
pp. 387-394 ◽  
Author(s):  
P. J. Tonge ◽  
V. E. Anderson ◽  
R. Fausto ◽  
M. Kim ◽  
M. Pusztai-Carey ◽  
...  
Keyword(s):  
Active Site ◽  
Electron Polarization ◽  
Raman Spectroscopic ◽  
Substrate Analog ◽  
Enoyl Coa Hydratase

Electronic Rearrangement Induced by Substrate Analog Binding to the Enoyl-CoA Hydratase Active Site: Evidence for Substrate Activation

Biochemistry ◽  
1994 ◽  
Vol 33 (42) ◽  
pp. 12635-12643 ◽  
Author(s):  
Robert L. D'Ordine ◽  
Peter J. Tonge ◽  
Paul R. Carey ◽  
Vernon E. Anderson
Keyword(s):  
Active Site ◽  
Substrate Activation ◽  
Substrate Analog ◽  
Enoyl Coa Hydratase

Crystal structure of enoyl-CoA hydratase from Thermus thermophilus HB8

2021 ◽  
Vol 77 (5) ◽  
pp. 148-155
Author(s):  
Sivaraman Padavattan ◽  
Sneha Jos ◽  
Hemanga Gogoi ◽  
Bagautdin Bagautdinov
Keyword(s):  
Crystal Structure ◽  
Active Site ◽  
Thermus Thermophilus ◽  
Tca Cycle ◽  
Significant Shift ◽  
Oxidation Pathway ◽  
Ligand Binding Sites ◽  
Chain Acyl ◽  
Α Helix ◽  
Enoyl Coa Hydratase

Fatty-acid degradation is an oxidative process that involves four enzymatic steps and is referred to as the β-oxidation pathway. During this process, long-chain acyl-CoAs are broken down into acetyl-CoA, which enters the mitochondrial tricarboxylic acid (TCA) cycle, resulting in the production of energy in the form of ATP. Enoyl-CoA hydratase (ECH) catalyzes the second step of the β-oxidation pathway by the syn addition of water to the double bond between C2 and C3 of a 2-trans-enoyl-CoA, resulting in the formation of a 3-hydroxyacyl CoA. Here, the crystal structure of ECH from Thermus thermophilus HB8 (TtECH) is reported at 2.85 Å resolution. TtECH forms a hexamer as a dimer of trimers, and wide clefts are uniquely formed between the two trimers. Although the overall structure of TtECH is similar to that of a hexameric ECH from Rattus norvegicus (RnECH), there is a significant shift in the positions of the helices and loops around the active-site region, which includes the replacement of a longer α3 helix with a shorter α-helix and 310-helix in RnECH. Additionally, one of the catalytic residues of RnECH, Glu144 (numbering based on the RnECH enzyme), is replaced by a glycine in TtECH, while the other catalytic residue Glu164, as well as Ala98 and Gly141 that stabilize the enolate intermediate, is conserved. Their putative ligand-binding sites and active-site residue compositions are dissimilar.

Substrate analog inhibition and active site titration of purified recombinant HIV-1 protease

Biochemistry ◽  
1990 ◽  
Vol 29 (1) ◽  
pp. 264-269 ◽  
Author(s):  
Alfredo G. Tomasselli ◽  
Mary K. Olsen ◽  
John O. Hui ◽  
Douglas J. Staples ◽  
Tomi K. Sawyer ◽  
...  
Keyword(s):  
Active Site ◽  
Substrate Analog ◽  
Hiv 1

ChemInform Abstract: SUBSTRATE ANALOG BINDING TO THE COUPLED BINUCLEAR COPPER ACTIVE SITE IN TYROSINASE

1985 ◽  
Vol 16 (43) ◽  
Author(s):  
D. E. WILCOX ◽  
A. G. PORRAS ◽  
Y. T. HWANG ◽  
K. LERCH ◽  
M. E. WINKLER ◽  
...  
Keyword(s):  
Active Site ◽  
Binuclear Copper ◽  
Substrate Analog

Substrate analog binding to the coupled binuclear copper active site in tyrosinase

1985 ◽  
Vol 107 (13) ◽  
pp. 4015-4027 ◽  
Author(s):  
Dean E. Wilcox ◽  
Arturo G. Porras ◽  
Yeong T. Hwang ◽  
Konrad Lerch ◽  
Marjorie E. Winkler ◽  
...  
Keyword(s):  
Active Site ◽  
Binuclear Copper ◽  
Substrate Analog

Synthesis of a new substrate analog of firefly luciferin. Active-site probe

Biochemistry ◽  
1973 ◽  
Vol 12 (10) ◽  
pp. 1845-1852 ◽  
Author(s):  
Lemuel J. Bowie ◽  
Vaclav Horak ◽  
Marlene DeLuca
Keyword(s):  
Active Site ◽  
Substrate Analog ◽  
Firefly Luciferin ◽  
Active Site Probe

Resonance Raman Spectroscopic Characterization of the Molybdopterin Active Site of DMSO Reductase

Biochemistry ◽  
1995 ◽  
Vol 34 (9) ◽  
pp. 3032-3039 ◽  
Author(s):  
L. Kilpatrick ◽  
K. V. Rajagopalan ◽  
J. Hilton ◽  
N. R. Bastian ◽  
E. I. Stiefel ◽  
...  
Keyword(s):  
Active Site ◽  
Resonance Raman ◽  
Spectroscopic Characterization ◽  
Dmso Reductase ◽  
Raman Spectroscopic
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