scholarly journals Roles of adenosine A 1 receptors in the regulation of SFK activity in the rat forebrain

2021 ◽  
Author(s):  
Li‐Min Mao ◽  
John Q. Wang
Keyword(s):  
Rat Forebrain

Inhibition by Lamotrigine of the Generation of Nitric Oxide in Rat Forebrain Slices

2002 ◽  
Vol 64 (2) ◽  
pp. 636-642 ◽  
Author(s):  
Ignacio Lizasoain ◽  
Richard G. Knowles ◽  
Salvador Moncada
Keyword(s):  
Nitric Oxide ◽  
Rat Forebrain

Calretinin-containing pathways in the rat forebrain

1995 ◽  
Vol 705 (1-2) ◽  
pp. 273-294 ◽  
Author(s):  
P. Krzywkowski ◽  
D.M. Jacobowitz ◽  
Y. Lamour
Keyword(s):  
Rat Forebrain

Visualisation of GABAergic neurons and synapses in the rat brain using immunohistochemistry for two forms of glutamate decarboxylase

2021 ◽  
Vol 21 (2) ◽  
pp. 63-73
Author(s):  
Valeria A. Razenkova ◽  
Dmitrii E. Korzhevskii
Keyword(s):  
Brain Tissue ◽  
Glutamate Decarboxylase ◽  
Brain Structures ◽  
Gabaergic Neurons ◽  
Laboratory Animals ◽  
Tissue Samples ◽  
Tissue Sections ◽  
Background Staining ◽  
The Central Nervous System ◽  
Rat Forebrain

BACKGROUND: Taking into account the importance of GABAergic brain system research and also the opportunity to achieve specific and accurate results in laboratory studies using immunohistochemical approaches, it seems important to have a reliable method of visualization GABA-synthesizing cells, their projections and synapses, for the morphofunctional analysis of GABAergic system both in normal conditions and in the experimental pathology. AIM: The aim of the study was to visualize analyze GABAergic neurons and synapses within rats brain using three different antibody types against glutamate decarboxylase and to identify the optimal conditions for reaction performing. MATERIALS AND METHODS: The study was performed on paraffin brain tissue sections of 5 adult Wistar rats. Immunohistochemical reactions using three antibody types against glutamate decarboxylase isoform 67 (GAD67) and glutamate decarboxylase isoform 65 (GAD65) were performed. Additional controls on C57/Bl6 mice and Chinchilla rabbits brain samples were also carried out. RESULTS: Antibodies used in the research made it possible to achieve high quality of GABAergic structures visualizing without increasing background staining. At the same time different antibody types are distinct in their efficacy to perform immunohistochemistry reaction on laboratory animal brain tissue samples. By performing additional controls, we discovered that there is necessary to adsorb secondary reagents immunoglobulins in order to eliminate nonspecific staining. It was found that GAD67 and GAD65 distribution in rat forebrain structures is different. It was stated that GAD67 immunohistochemistry most completely reveals GABAergic brain structures compared to GAD65 immunhistochemistry. The possibility of determining morphological features of GABAergic neurons and synaptic terminals, as well as performing quantitative analysis, was demonstrated. CONCLUSIONS: The approach proposed makes it possible to specifically visualize GABAergic structures of the central nervous system of different laboratory animals. This could be useful both in fundamental studies and in pathology research.

Characteristic features of specific binding of pentapeptide HFPGP labeled at the C-terminal proline residue to rat forebrain plasma membranes

2014 ◽  
Vol 456 (1) ◽  
pp. 101-103 ◽  
Author(s):  
T. V. Vyunova ◽  
L. A. Andreeva ◽  
K. V. Shevchenko ◽  
V. P. Shevchenko ◽  
M. Yu. Bobrov ◽  
...  
Keyword(s):  
Plasma Membranes ◽  
Specific Binding ◽  
Proline Residue ◽  
Rat Forebrain ◽  
Characteristic Features

A comparison of Δ9-THC and anandamide induced c-fos expression in the rat forebrain

1998 ◽  
Vol 802 (1-2) ◽  
pp. 19-26 ◽  
Author(s):  
Iain S. McGregor ◽  
Jonathon C. Arnold ◽  
Marianne F. Weber ◽  
Ann N. Topple ◽  
Glenn E. Hunt
Keyword(s):  
Rat Forebrain ◽  
Fos Expression
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