Framework for the Rapid Optimization of Soluble Protein Expression inEscherichia coliCombining Microscale Experiments and Statistical Experimental Design

2007 ◽  
Vol 23 (4) ◽  
pp. 785-793 ◽  
Author(s):  
R. S. Islam ◽  
D. Tisi ◽  
M. S. Levy ◽  
G. J. Lye
Keyword(s):  
Experimental Design ◽  
Protein Expression ◽  
Soluble Protein ◽  
Statistical Experimental Design

scholarly journals Optimization of rPDT fusion protein expression by Escherichia coli in pilot scale fermentation: a statistical experimental design approach

AMB Express ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Nasser Nassiri Koopaei ◽  
Parissa Khadiv-Parsi ◽  
Mohammad Reza Khoshayand ◽  
Mohammad Ali Mazlomi ◽  
Abbas Kebriaeezadeh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Experimental Design ◽  
Fusion Protein ◽  
Protein Expression ◽  
Pilot Scale ◽  
Design Approach ◽  
Statistical Experimental Design ◽  
Fusion Protein Expression

Adenovirus VA RNAI: a positive regulator of mRNA translation

1984 ◽  
Vol 4 (4) ◽  
pp. 736-742
Author(s):  
C Svensson ◽  
G Akusjärvi
Keyword(s):  
Experimental Design ◽  
Protein Expression ◽  
Mrna Translation ◽  
Dna Binding Protein ◽  
Transient Expression Assay ◽  
Transfected Cells ◽  
Cell Extracts ◽  
293 Cells ◽  
The Common ◽  
Monospecific Antisera

We have developed a sensitive transient expression assay in 293 cells to study the effect of VA RNAs on the translation of adenovirus mRNAs. Monolayers of 293 cells were transfected with mixtures of recombinant plasmids encoding adenovirus-specific transcription units and plasmids encoding VA RNAs. Transfected cells were labeled with [35S]methionine for ca. 15 h, and labeled cell extracts were prepared. Changes in the protein expression caused by VA RNA cotransfection were measured by immunoprecipitation, using monospecific antisera prepared against adenovirus-specific polypeptides. Using this experimental design, we demonstrate that VA RNAI stimulates the translation of both early and late adenovirus mRNAs. Synthesis of the E3 19,000-dalton glycoprotein and the E2A 72,000-dalton DNA binding protein was stimulated between 10 and 20 times by VA RNAI cotransfection. Synthesis of the late hexon polypeptide was also stimulated, although translation of hexon was from an aberrant mRNA lacking the second and third segments of the common tripartite leader attached to late adenovirus mRNAs. VA RNAII, although very homologous to VA RNAI, does not function as a translational stimulator.

A Statistical Experimental Design to Plastein Synthesis with the Mixture of Soy Protein Isolate(SPI) Hydrolysate and Whey Protein Isolate(WPI) Hydrolysate

2011 ◽  
Vol 236-238 ◽  
pp. 2773-2779
Author(s):  
Ying Cao Xu ◽  
Zhi Biao Feng ◽  
Chun Hong Liu
Keyword(s):  
Experimental Design ◽  
Whey Protein ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Statistical Experimental Design ◽  
Operating Variables ◽  
Enzyme Substrate ◽  
Predicted Values

A statistical experimental design to plastein synthesis which was catalyzed by transglutaminase, using the mixture of soy protein isolate(SPI) hydrolysate and whey protein isolate (WPI) hydrolysate, was investigated. Enzyme/Substrate(E/S:5-25U/g), pH(5-9) and temperature (35-65°C) were selected as major operating variables. To investigate the effects of variables to yield of plastein, the statistical experiment of Box-Behnken design(BBD) and Response Surface methodology(RSM) was employed. Regression analysis showed that the experiment data accorded with the predicted values obtained from quadratic regression equation in BBD with R-Squared of 0.9866 and F-value of 102.51. The optimum results estimated by BBD were as follows: E/S(19.5U/g), pH(6.8), and temperature(50.0°C), gave a maximum plastein yield of 54%. In the present experiment, the preliminary study on plastein functions such as foaming, emulsifying, were showed that plastein had a good biological function.

Chitinase Production in Solid-State Fermentation by Enterobacter sp. NRG4 Using Statistical Experimental Design

2005 ◽  
Vol 51 (4) ◽  
pp. 222-228 ◽  
Author(s):  
Neetu Dahiya ◽  
Rupinder Tewari ◽  
Ram Prakash Tiwari ◽  
Gurinder Singh Hoondal
Keyword(s):  
Solid State ◽  
Experimental Design ◽  
Solid State Fermentation ◽  
Statistical Experimental Design ◽  
Chitinase Production
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