Fomblin as a reference compound for negative ion fast atom bombardment high-resolution mass spectrometry in the mass range of 180-3000 u

1993 ◽  
Vol 22 (3) ◽  
pp. 170-175 ◽  
Author(s):  
Longfei Jiang ◽  
Mehdi Moini
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Fast Atom Bombardment ◽  
High Resolution Mass Spectrometry ◽  
Mass Range ◽  
Negative Ion ◽  
Reference Compound ◽  
High Resolution Mass ◽  
Resolution Mass

scholarly journals Determination of Atrazine and Hydroxyatrazine in Agricultural Runoff Waters by Liquid Chromatography and Fast Atom Bombardment-High Resolution Mass Spectrometry

1996 ◽  
Vol 79 (4) ◽  
pp. 929-935 ◽  
Author(s):  
Zongwei Cai ◽  
Steven J Monson ◽  
Roy F Spalding
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Water Samples ◽  
Fast Atom Bombardment ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
Runoff Events ◽  
Resolution Mass

Abstract Agricultural surface water samples were collected after the first 2 major runoff events in 1994 for determination of atrazine (ATR, 2-chloro-4-ethylamino-6-isopropylamino-s-triazine) and hydroxyatrazine (HA, 2-ethylamino-4-hydroxy-6-isopropylamino-striazine). After addition of. 13C3-labeled ATR and 13C3-labeled HA as internal standards, the chemicals were quantitatively extracted and eluted from water samples by liquid-solid extraction with a carbon black cartridge. Liquid chromatography and fast atom bombardment-high resolution mass spectrometry were used to analyze the sample extracts. Concentrations of ATR ranged from 8.2 to 15.1 parts per billion (ppb) in the samples collected after both runoff events from a highly agricultural watershed in south-central Nebraska. HA concentrations ranged from 0.25 to 2.7 ppb in the water samples. Results obtained from both analytical procedures agreed within a 15% deviation. As predicted from runoff events, the highest levels occurred in samples from the first collection on June 24,1994, for both ATR and HA, whereas samples collected on July 5,1994, contained much lower levels of HA.

scholarly journals Differential Analysis of Serum Principal Components Treated with Compound Sophora Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS)

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Wanjin Sun ◽  
Junjie Zhang ◽  
Conghui Zhou ◽  
Bin Yan ◽  
Quan Cai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Principal Components ◽  
Collision Energy ◽  
High Resolution Mass Spectrometry ◽  
Ion Source ◽  
Negative Ion ◽  
Model Group ◽  
High Resolution Mass ◽  
Resolution Mass

Objective. To compare the differences in the serum principal components in ulcerative colitis- (UC-) induced rats, treated with compound Sophora decoction, matrine, oxymatrine monomer mixture, and indirubin monomer, and to provide a modern scientific basis for elucidating the clinical efficacy of compound Sophora decoction for the treatment of UC. Methods. The serum samples of rats from each group were obtained after drug administration, and the serum principal components of each group were analyzed by high-resolution mass spectrometry. Agilent Eclipse XDB C18 chromatographic column (100 mm × 2.1 mm, 3.5 m) was used for separation. The mobile phase was water (A) and methanol (B) (0.1% formic acid) gradient elution, 0–3 min (B: 20%–40%), 3–10 min (B: 40%–54%), 10–25 min (B: 54%), 25–35 min (B: 54%–70%), 35–45 min (B: 70%–80%), 45–50 min (B: 80%), 50–60 min (B: 80%–100%), 70–72 min (B: 100%–20%), and 72–77 min (B: 20%); flow rate, 300 μL/min; column temperature, 40°C; and injection volume, 10 μL. ESI source was selected and scanned in the positive and negative ion modes. The scanning range was 70–1500 m/z; ion-source gas 1 (GS1): 55 psi; ion-source gas 2 (GS2): 60 psi; CUR: 30 psi; ion-source temperature (TEM): 550°C; ion-source voltage (ISVF) : 5500 V/−4500 V; decluster voltage (DP): 100 V; collision energy (CE): 35 V/−35 V; collision energy gain (CES) : 15 V/−15 V; and data acquisition mode: IDA. After the data from each group were imported into MarkView 1.3, the molecular weights and retention times of different substances were obtained and qualitatively analyzed by ChemSpider and PeakView 2.0. Results. In the negative ion mode, 26 differential compounds were identified in the compound Sophora decoction group (FFKST) compared to the model group (M), and 18 differential compounds were identified in the matrine and oxymatrine group (KST) compared to the model group (M). In the positive ion mode, 11 and 7 differential compounds were identified in the compound Sophora decoction group (FFKST) and the matrine and oxymatrine group (KST) compared to the model group (M), respectively. The responses of all compounds in each group were compared with each other. As the different principal component substances in the indirubin group (DYH) displayed little correlation with other groups, the different components in this group were not researched thoroughly. Conclusion. By comparing the differences in the serum principal components from each administration group, we found that the FFKST group exhibited enhanced synthesis of the serum principal components; however, the compound doses of matrine and oxymatrine monomers did not exhibit the same changes in the serum principal components of UC-induced rats. Finally, the traditional Chinese medicine compound is more advantageous than monomers.

Application of quadrupole-Orbitrap high-resolution mass spectrometry in rapid screening and identification of synthetic dyes in herbal medicines

2019 ◽  
Vol 25 (5) ◽  
pp. 419-427
Author(s):  
Changchuan Guo ◽  
Ke Li ◽  
Sheng Xing ◽  
Hua Sun ◽  
Feng Shi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Herbal Medicines ◽  
Negative Ion ◽  
Rapid Screening ◽  
Synthetic Dyes ◽  
High Resolution Mass ◽  
Resolution Mass ◽  
Identification And Quantification

In this work, a method combining ultra-high performance liquid chromatography and hybrid quadrupole-Orbitrap high-resolution mass spectrometry (HR-MS) was developed and validated for use in the simultaneous screening, identification, and quantification of 21 synthetic dyes in herbal medicines. To optimize the chromatographic conditions, we used a combined Full mass scan and data-dependent MS/MS (Full MS/dd-MS2) approach in positive and negative ion mode. Under this mode, selected ions with given fragmentation energy were subjected to a dd-MS2 scan following a Full MS scan. The selectivity of this method was effectively improved using 70,000 full width at half maximum mass resolution and narrow mass window (typically 5 ppm), and a single injection was sufficient for simultaneous identification and quantification of 21 synthetic dyes within 10 min. The combined method was fully validated and complies with all criteria for selectivity, sensitivity, calibration curve linearity, accuracy, precision, recovery, matrix effect, and stability. All analytes showed excellent linear relationships as all the coefficients of determination ( r2) are greater than 0.9978 over wide ranges of concentrations (e.g. 1.0–500 ng/mL for sunset yellow). The validated method was employed to detect synthetic dyes in herbal medicines and was demonstrated to provide a reliable technical basis for drug regulation and public health protection.

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