Analysis of platelet activating factor in human saliva by gas chromatography/mass spectrometry

1989 ◽  
Vol 18 (4) ◽  
pp. 258-264 ◽  
Author(s):  
Brian W. Christman ◽  
Ian A. Blair
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Platelet Activating Factor ◽  
Human Saliva ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Quantification of homocysteine thiolactone in human saliva and urine by gas chromatography-mass spectrometry

2020 ◽  
Vol 1149 ◽  
pp. 122155 ◽  
Author(s):  
Justyna Piechocka ◽  
Monika Wrońska ◽  
Grażyna Chwatko ◽  
Hieronim Jakubowski ◽  
Rafał Głowacki
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Human Saliva ◽  
Gas Chromatography Mass Spectrometry ◽  
Homocysteine Thiolactone ◽  
Chromatography Mass Spectrometry

scholarly journals Gas Chromatography–Mass Spectrometry Based Approach for the Determination of Methionine-Related Sulfur-Containing Compounds in Human Saliva

2020 ◽  
Vol 21 (23) ◽  
pp. 9252
Author(s):  
Justyna Piechocka ◽  
Monika Wieczorek ◽  
Rafał Głowacki
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Human Saliva ◽  
Gas Chromatography Mass Spectrometry ◽  
Limit Of Quantification ◽  
Homocysteine Thiolactone ◽  
Chromatography Mass Spectrometry ◽  
First Choice ◽  
Sulfur Containing ◽  
Sulfur Containing Compounds

Gas chromatography–mass spectrometry technique (GC-MS) is mainly recognized as a tool of first choice when volatile compounds are determined. Here, we provide the credible evidence that its application in analysis can be extended to non-volatile sulfur-containing compounds, to which methionine (Met), homocysteine (Hcy), homocysteine thiolactone (HTL), and cysteine (Cys) belong. To prove this point, the first method, based on GC-MS, for the identification and quantification of Met-related compounds in human saliva, has been elaborated. The assay involves simultaneous disulfides reduction with tris(2-carboxyethyl)phosphine (TCEP) and acetonitrile (MeCN) deproteinization, followed by preconcentration by drying under vacuum and treatment of the residue with a derivatizing mixture containing anhydrous pyridine, N-trimethylsilyl-N-methyl trifluoroacetamide (MSTFA), and trimethylchlorosilane (TMCS). The validity of the method was demonstrated based upon US FDA recommendations. The assay linearity was observed over the range of 0.5–20 µmol L−1 for Met, Hcy, Cys, and 1–20 µmol L−1 for HTL in saliva. The limit of quantification (LOQ) equals 0.1 µmol L−1 for Met, Hcy, Cys, while its value for HTL was 0.05 µmol L−1. The method was successfully applied to saliva samples donated by apparently healthy volunteers (n = 10).

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