Fast atom bombardment and fast atom bombardment collision-activated dissociation/mass-analysed ion kinetic energy analysis ofC-glycosidic flavonoids

1989 ◽  
Vol 18 (2) ◽  
pp. 122-130 ◽  
Author(s):  
M. Becchi ◽  
D. Fraisse
Keyword(s):  
Kinetic Energy ◽  
Fast Atom Bombardment ◽  
Energy Analysis ◽  
Ion Kinetic Energy

Differentiation of anomeric unsaturated C-glycosides by fast atom bombardment ionisation-collision activated dissociation/mass-analyzed ion kinetic energy analysis

1990 ◽  
Vol 25 (4) ◽  
pp. 249-252 ◽  
Author(s):  
Mohamed Brakta ◽  
Paul Lhoste ◽  
Denis Sinou ◽  
Michel Becchi ◽  
Daniel Fraisse ◽  
...  
Keyword(s):  
Kinetic Energy ◽  
Fast Atom Bombardment ◽  
Energy Analysis ◽  
Ion Kinetic Energy

Angle-dependence of ion kinetic energy spectra obtained by using mass spectrometers II. Experimental considerations and preliminary results on non-fragmenting systems

1984 ◽  
Vol 392 (1802) ◽  
pp. 89-106 ◽  
Keyword(s):  
Kinetic Energy ◽  
Energy Analysis ◽  
Energy Spectra ◽  
Observation Angle ◽  
Initial Angle ◽  
Mass Spectrometers ◽  
Small Uncertainty ◽  
Ion Kinetic Energy ◽  
Double Focusing ◽  
Selection Of

Experimental problems associated with studies of the scattering of kilovolt projectile ions in mass spectrometers designed primarily for chemical analysis are discussed. The parameters that are important in satisfactorily controlling and defining the actual scattering angle in relation to the observation angle are considered in detail. Two different experimental configurations are considered; angular selection before, or after kinetic energy analysis of the collision products. A modification to a VG ZAB-2F double-focusing mass spectrometer, in order to observe angle-resolved ion kinetic energy spectra of collisionally scattered ion beams, is described. Initial angle-resolved experiments on systems that do not involve fragmentation, have been performed. The results are presented for the angle-resolved energy loss spectra of Ar + and N 2 + and the angle-resolved charge-stripping of Ar + . A feature of the apparatus described is the small uncertainty in the angular selection of the analysing angle-resolved slit.

scholarly journals Extraction, purification and identification of cytidine 3′,5′-cyclic monophosphate from rat tissues

1984 ◽  
Vol 221 (3) ◽  
pp. 665-673 ◽  
Author(s):  
R P Newton ◽  
S G Salih ◽  
B J Salvage ◽  
E E Kingston
Keyword(s):  
Kinetic Energy ◽  
Large Scale ◽  
Fast Atom Bombardment ◽  
Paper Electrophoresis ◽  
Rat Tissues ◽  
Extraction And Purification ◽  
Unequivocal Identification ◽  
Ion Kinetic Energy ◽  
Radio Immunoassay ◽  
Cyclic Cmp

The large-scale extraction and purification to homogeneity of cyclic CMP and its unequivocal identification are described. Rat liver, kidney, heart, spleen and lung tissues were subjected to a sequential purification procedure involving freeze-clamping, perchlorate extraction, alumina and boronate column chromatography, polyacrylamide-gel column electrophoresis and high-voltage paper electrophoresis. The purified sample co-chromatographed with authentic cyclic CMP on t.l.c. and high-pressure liquid chromatography and was positive in a cyclic CMP radio-immunoassay. The u.v., i.r. and p.m.r. spectra were each essentially identical with those of authentic cyclic CMP. Fast-atom bombardment of authentic cyclic CMP yielded a mass spectrum containing a molecular protonated ion: mass-ion-kinetic-energy scanning of this ion produced a spectrum unique to 3′,5′-cyclic CMP. The extracted nucleotide produced an identical mass-ion-kinetic-energy spectrum.

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