The amino acid sequences of the tryptic peptides of cowpea chlorotic mottle virus protein

1980 ◽  
Vol 7 (3) ◽  
pp. 132-138 ◽  
Author(s):  
M. W. Rees ◽  
R. Casey ◽  
Margaret N. Short ◽  
Janice J. Sexton ◽  
J. F. March ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequences ◽  
Mottle Virus ◽  
Virus Protein ◽  
Tryptic Peptides ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals Intrinsic Fluorescene of Cowpea-Chlorotic-Mottle-Virus Protein

1979 ◽  
Vol 95 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Jacob KRUSE ◽  
Benedictus J. M. VERDUIN ◽  
Antonie J. W. G. VISSER
Keyword(s):  
Mottle Virus ◽  
Virus Protein ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

scholarly journals Symptom induction by Cowpea chlorotic mottle virus on Vigna unguiculata is determined by amino acid residue 151 in the coat protein

2002 ◽  
Vol 83 (4) ◽  
pp. 879-883 ◽  
Author(s):  
F. M. de Assis Filho ◽  
O. R. Paguio ◽  
J. L. Sherwood ◽  
C. M. Deom
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Vigna Unguiculata ◽  
Amino Acid Residue ◽  
Site Directed Mutagenesis ◽  
Mottle Virus ◽  
Cdna Clones ◽  
Cowpea Chlorotic Mottle Virus ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle

The type strain of Cowpea chlorotic mottle virus (CCMV-T) produces a bright chlorosis in cowpea (Vigna unguiculata cv. California Blackeye). The attenuated variant (CCMV-M) induces mild green mottle symptoms that were previously mapped to RNA 3. Restriction fragment exchanges between RNA 3 cDNA clones of CCMV-T and CCMV-M that generate infectious transcripts and site-directed mutagenesis indicated that the codon encoding amino acid residue 151 of the coat protein determines the symptom phenotypes of CCMV-T and CCMV-M. Amino acid 151 is within an α-helical structure required for calcium ion binding and virus particle stability. No differences in virion stability or accumulation were detected between CCMV-T and CCMV-M. Mutational analysis suggested that the amino acid at position 151 and not the nucleotide sequence induce the symptom phenotype. Thus, it is likely that subtle influences by amino acid residue 151 in coat protein–host interactions result in chlorotic and mild green mottle symptoms.

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