Estimation of nitrogen-15 levels in derivatized amino acids using gas chromatography quadrupole mass spectrometry with chemical ionization and selected ion monitoring

1978 ◽  
Vol 5 (12) ◽  
pp. 648-653 ◽  
Author(s):  
J. R. Robinson ◽  
A. N. Starratt ◽  
E. E. Schlahetka
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Gas Chromatography ◽  
Chemical Ionization ◽  
Quadrupole Mass ◽  
Selected Ion Monitoring ◽  
Quadrupole Mass Spectrometry

Metabolic profiling of 19 amino acids in triptolide-induced liver injured rats by gas chromatography-triple quadrupole mass spectrometry

2021 ◽  
pp. 096032712110061
Author(s):  
Cong Hu ◽  
Hongwei Li ◽  
Linjing Wu ◽  
Jiaqun Ke ◽  
Xuechun Yu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Gas Chromatography ◽  
Amino Acid ◽  
Metabolic Profiling ◽  
Triple Quadrupole ◽  
Glutamate Metabolism ◽  
Quadrupole Mass ◽  
Triple Quadrupole Mass Spectrometry ◽  
Quadrupole Mass Spectrometry

The liver is an important organ for amino acid metabolism, and its damage can be reflected in the changes of amino acid level in the body. Triptolide (TP) has broad anti-inflammatory and anti-tumor activities, but its clinical application is limited due to hepatotoxicity. In this work, a simple, accurate and sensitive gas chromatography-triple quadrupole mass spectrometry (GC-QqQ-MS/MS) method was developed and validated for evaluating the serum levels of amino acids from control and TP-induced liver injured rats, and chemometric analysis was employed for amino acid metabolic profiles analysis. It was found that 11 amino acids showed significant changes after TP administration, and they were mainly involved in 5 metabolic pathways that are phenylalanine, tyrosine and tryptophan biosynthesis, alanine, aspartate and glutamate metabolism, glutamine and glutamate metabolism, phenylalanine metabolism and arginine biosynthesis. Five amino acids including tyrosine, glutamine, glutamic acid, tryptophan and alanine were identified as biomarkers of TP hepatotoxicity by further analysis. These results indicated that the novel amino acid metabolic profiling study based on the GC-QqQ-MS/MS provided not only exact concentrations of serum amino acids, but also a prospective methodology for evaluation of chemically induced liver injury.

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