A simple and cost effective method for the quantification of 8-hydroxy-2′-deoxyguanosine from urine using liquid chromatography tandem mass spectrometry

2008 ◽  
Vol 22 (4) ◽  
pp. 394-401 ◽  
Author(s):  
Brian Crow ◽  
Michael Bishop ◽  
Kasey Kovalcik ◽  
Dean Norton ◽  
Joe George ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Cost Effective ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Cost Effective Method ◽  
Liquid Chromatography Tandem Mass

Liquid Chromatography–Tandem Mass Spectrometry–Based α1-Antitrypsin (AAT) Testing

2020 ◽  
Author(s):  
Josiah D Murray ◽  
Maria A Willrich ◽  
Michael J Krowka ◽  
Aleh Bobr ◽  
David L Murray ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Cost Effective ◽  
Vital Role ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Retrospective Data Analysis ◽  
Cost Effective Approach ◽  
Liquid Chromatography Tandem Mass

Abstract Objectives Failure to produce sufficient quantities of functional α1-antitrypsin (AAT) can result in AAT deficiency (AATD) and significant comorbidities. Laboratory testing plays a vital role in AATD, with diagnosis requiring documentation of both a low AAT level and a mutated allele. This retrospective evaluation examines the efficacy of a liquid chromatography–tandem mass spectrometry (LC-MS/MS) (proteotyping)–based algorithm for AATD detection. Methods A 16-month retrospective data analysis was performed on two cohorts: 5,474 samples tested with the proteotype-based algorithm and 16,147 samples directly tested by isoelectric focusing (IEF) phenotyping. Results LC-MS/MS reduced the rate of IEF testing by 97%. The 3% of cases reflexed to IEF resulted in 12 (0.2%) additional phenotype findings. Retrospectively applying the proteotype-based algorithm to the IEF cohort demonstrated a 99.9% sensitivity for the detection of deficiency-associated phenotypes. Most deficiency phenotypes missed by the proteotyping algorithm would come from heterozygous patients with an F, I, or P paired to an S or Z. In all of these cases, patient AAT levels were greater than 70 mg/dL, above the threshold for AAT augmentation therapy. Conclusions The proteotype algorithm is a sensitive and cost-effective approach for the diagnosis of clinical AAT deficiency.

scholarly journals Quantitative Measurement of Plasma Free Metanephrines by a Simple and Cost-Effective Microextraction Packed Sorbent with Porous Graphitic Carbon and Liquid Chromatography-Tandem Mass Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Xin Xiong ◽  
Yuanyuan Zhang ◽  
Rongsheng Zhao
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Quantitative Measurement ◽  
Cost Effective ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Plasma Free Metanephrines ◽  
Packed Sorbent

Plasma free metanephrines are widely used for the diagnosis of pheochromocytoma and paraganglioma (PPGL), yet quantifying metanephrines using a simple and cost-effective approach may be challenging due to preanalytical and analytical constraints. In this study, we established and validated a new method for quantitative measurement of plasma free metanephrines based on microextraction by packed sorbent (MEPS) with porous graphitic carbon (PGC) and liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The elution step was fully compatible with HILIC mode without evaporation and reconstitution. The analytes were well resolved, and potential interferences (54 substances) were investigated. This method was linear from 24.7–2717 pg/mL for metanephrine (MN) and 24.5–4010 pg/mL for normetanephrine (NMN) with a coefficient of determination (R2) higher than 0.994. The limit of MN and NMN detection were 12.4 pg/mL and 12.3 pg/mL, respectively. The intra- and interassay impressions were ≤12.8% for spiked quality controls and ≤13.6% for commercial quality controls; the method recoveries ranged within 88.0–109.0%, respectively. The area under the receiver operating characteristic (ROC) curve was 0.848 ± 0.047 for MN and 0.979 ± 0.021 for NMN. Validation that was performed by comparing clinical specimens with various biochemical results showed that plasma free metanephrines in a seated position had comparable sensitivity and lower specificity to urinary free metanephrines, which could be compensated by combining other biochemical tests. The newly developed MEPS method resulted as a time-saving, reliable, and cost-effective microextraction technique that can be applied for a successful screening of PPGL.

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