Identification of linoleic acid free radicals and other breakdown products using spin trapping with liquid chromatography-electrospray tandem mass spectrometry

2005 ◽  
Vol 20 (1) ◽  
pp. 109-118 ◽  
Author(s):  
A. Reis ◽  
M. R. M. Domingues ◽  
F. M. L. Amado ◽  
A. J. V. Ferrer-Correia ◽  
P. Domingues
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Free Radicals ◽  
Linoleic Acid ◽  
Tandem Mass Spectrometry ◽  
Spin Trapping ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry

Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry

2017 ◽  
Vol 10 (1) ◽  
pp. 100-109
Author(s):  
Hongxia Li ◽  
Natasha Trzaskalski ◽  
R.J. Neil Emery
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Soybean Seeds ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry ◽  
Aqueous Methanol ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Objective:A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.Materials:The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.Method:Five different purification strategies have been tested for the purification and enrichment of BRs.Conclusion:This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.

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